Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,112,861	C→T	R67R (CGG→CGA)	rfbB ←	dTDP‑glucose 4,6‑dehydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,112,861	0	C	T	100.0%	90.7 / NA	26	R67R (CGG→CGA)	rfbB	dTDP‑glucose 4,6‑dehydratase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/12);  total (14/12)

CATCTGGATGAGGCAAATCACCATAGACTTCGTCAGTAGAAATATGATGAAAACGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATCCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGC  >  NC_000913/2112629‑2113104                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    cATCTGGATGAGGCAAATCACCATAGGCTTCGTCAGTAGAAATATGATGAAAACGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGc                                                                                                                                                                                                                                    <  1:404486/250‑1 (MQ=255)        aTGAGGCAAATCACCATAGACTTCGTCAGTAGAAATATGATGAAAACGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCaa                                                                                                                                                                                                                             >  2:137247/1‑250 (MQ=255)             gCAAATCACCATAGACTTCGTCAGTAGAAATATGATGAAAACGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATcc                                                                                                                                                                                                                       <  2:383961/251‑1 (MQ=255)                aaTCACCATAGACTTCGTCAGTAGAAATATGATGAAAACGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGACTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGAGCATCACTGCATCCGGATGATGCTGAGCAAAAATTCGTGCCATTGAAGGTGCATCGCAAATATCCGc                                                                                                                                                                                                                     >  2:256386/1‑250 (MQ=255)                             ttCGTCAGTAGAAATATGATGAAAACGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACAt                                                                                                                                                                                                       >  1:330826/1‑251 (MQ=255)                                    gTAGAAATATGATGAAAACGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTtc                                                                                                                                                                                                >  2:293217/1‑251 (MQ=255)                                                   aaaCGGAAGCTCTTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATc                                                                                                                                                                                 >  2:264171/1‑251 (MQ=255)                                                                         cGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCg                                                                                                                                                           <  1:293217/251‑1 (MQ=255)                                                                             aTCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGt                                                                                                                                                       <  2:289721/251‑1 (MQ=255)                                                                                                                   ggACATAAGTACCAACAATAATGGTTTCAATAAATGCCGCAGGGCCTGTAATTGATCGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGta                                                                                                                 <  2:59871/251‑1 (MQ=255)                                                                                                                      cATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGtatt                                                                                                               >  2:152638/1‑250 (MQ=255)                                                                                                                                     tatTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACg                                                                                                >  2:37038/1‑250 (MQ=255)                                                                                                                                              cAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTattatt                                                                                                            >  1:31868/1‑229 (MQ=255)                                                                                                                                              cAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTattatt                                                                                                            <  2:31868/229‑1 (MQ=255)                                                                                                                                                  aaaTGCCGCAGGGCCTGTAATTGACCGGTCAACATGGATTTCAGCAGCCAGGTGCATCACTGCATCCGCCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAAc                                                                                  >  2:422550/1‑251 (MQ=255)                                                                                                                                                      gCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCa                                                                                <  1:252147/249‑1 (MQ=255)                                                                                                                                                      gCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATAGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCaa                                                                               >  2:252147/1‑250 (MQ=255)                                                                                                                                                               ccTGTAATTGAACGGTCAACATGGCTTTCAGCAGCAAGGTGCATCACTGCATCCGGATGATGCTGAGCAAAAATTCGAGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTgc                                                                     >  1:256436/1‑251 (MQ=255)                                                                                                                                                                  gTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACGATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTgcgc                                                                   <  1:37038/250‑1 (MQ=255)                                                                                                                                                                        gAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGt                                                            >  1:288478/1‑251 (MQ=255)                                                                                                                                                                               cAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAg                                                      <  2:335381/250‑1 (MQ=255)                                                                                                                                                                                             gcagcCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTGTCACTTAAtt                                       <  1:380518/251‑1 (MQ=255)                                                                                                                                                                                                 ccAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTAtt                                   >  2:427148/1‑251 (MQ=255)                                                                                                                                                                                                                                aGCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATa     >  2:246011/1‑250 (MQ=255)                                                                                                                                                                                                                                 gCAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCGTGCTGTGAAATAAg   <  1:130261/251‑1 (MQ=255)                                                                                                                                                                                                                                  cAAAAATTCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGc  <  1:23241/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    CATCTGGATGAGGCAAATCACCATAGACTTCGTCAGTAGAAATATGATGAAAACGGAAGCTATTTTTCTTGTCGCTATCAAGAGCAGACCAGTAATTGCGAGCGGCTTCCAAAAGGACATAAGTACCAACAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATCCGTGCCATTGCAGGTGCATCGCAAATATCCGCATGTTCAAAAACATAGCGTTCAGAATCAGAAACATCAGCAAGTGATTCCCGGTTTCCGGCGTACGTTAATTTATCGACATTAACAACACTATCCTGCGTATTATTTATAATGTGACGAACTACAGCTGAACCAATAAATCCTGCGCCACCAGTAACAAGTATTTTCACTTAATTTATTCCATATTACTTCAGAGCATGCTGTGAAATAAGC  >  NC_000913/2112629‑2113104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑