Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	856,923	G→A	E321K (GAA→AAA)	ybiT →	putative ATP‑binding protein YbiT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	856,923	0	G	A	100.0%	105.4 / NA	31	E321K (GAA→AAA)	ybiT	putative ATP‑binding protein YbiT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/15);  total (16/15)

GTGAACGTCTGCTGGCCGATAACGCCAAGAAGAAAGCGCAGATTGCTGAGTTGCAATCTTTCGTTAGCCGCTTTAGCGCCAACGCCTCGAAATCTCGCCAGGCAACTTCGCGCGCGCGCCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGGAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGT  >  NC_000913/856705‑857166                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    gTGAACGTCTGCTGGCCGATAACGCCAAGAAGAAAGCGCAGATTGCTGAGTTGCAATCTTTCGTTAGCCGCTTTAGCGCCAACGCCTCGAAATCTCGCCAGGCAACTTCGCGCGCGCGCCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAAc                                                                                                                                                                                                                     >  1:353663/1‑251 (MQ=255)                         ccAAGAAGAAAGCGCAGATTGCTGAGTTGCAATCTTTCGTTAGCCGCTTTAGCGCCAACGCCTCGAAATCTCGCCAGGCAACTTCGCGCGCGCGCCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAAc                                                                                                                                                                                             >  1:431703/1‑251 (MQ=255)                                              cTGAGTTGCAATCTCTCGTTAGCCGCTTTAGCGCCAACGCCTCGAAATCTCGACAGGCAAATTTGCGCGCGCGCCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAAACCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCAGCTGCTGGAAGTGGGTGa                                                                                                                                                                         >  2:423313/1‑250 (MQ=255)                                                 ggttgcaatctgtcggtgtcggCTTTAGCGCCAACTCCTCGAAATCTCGACAGACATCTTCGCGTGCGCGCCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGGCAGAACCCGTTCATCCGTTTTGAGCAGGATAAGAAACTGTTCCTTAACGCTCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTTTTTAAAAATCTAAACCTGCTGTGGGGAGTGGGTGaaaaa                                                                                                                                                                     <  2:457663/230‑1 (MQ=255)                                                                         ttAGCGCCAACGCCTCGAAATCTCGCCAGGCAACTTCGCGCGCGCGCCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGc                                                                                                                                             >  1:455123/1‑251 (MQ=255)                                                                                     ccTCGAAATCTCGCCAGGCAACTTCGCGCGCGCGCCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCa                                                                                                                                 <  1:231123/251‑1 (MQ=255)                                                                                      cTCGAAATCTCGCCAGGCAACTTCGCGCGCGCGCCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGGCGGTAAATcc                                                                                                                                 >  2:166142/1‑249 (MQ=255)                                                                                                    aGGCAACTTCGCGCGCGCGCCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAACAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGGACCAACGGCGGCGGTAAATCACCGCTGCTGAAAAcc                                                                                                                  >  2:207847/1‑250 (MQ=255)                                                                                                            tCGCGCGCGCGCCAGATTGATAAAATCAAACTGGAAGCGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACTGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGCGAAAAACTGGAGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGTTGGTggg                                                                                                          >  1:426046/1‑251 (MQ=255)                                                                                                             cgcgcgcgcgCCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGTCGGTACTGGGTTCCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGc                                                                                                         <  1:432306/251‑1 (MQ=255)                                                                                                               cgcgcgcgcCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCg                                                                                                        >  2:452947/1‑250 (MQ=255)                                                                                                                 cgcgcgcCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGTGTTTGATAACGGTCCGCTGGTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATc                                                                                                     <  1:231002/251‑1 (MQ=255)                                                                                                                     cgcCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCa                                                                                                 <  2:64818/251‑1 (MQ=255)                                                                                                                           aTTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCgg                                                                                            >  1:332401/1‑250 (MQ=255)                                                                                                                           aTTGATAAAATCAAACTGGAAAAGGCGAAAACCTCAAACCGAAAGACCCCGTTCATCCGTTTTGAACAGGATAAGAATCAGTTCCGTAACGAGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCGGATGTTTAACAATATCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGGACGGGGTCCCCATCGCGTCAGTAAATGAACGCTGATGAAAAAGCTGGGGGGGGACCCGCAACNgg                                                                                            >  2:407530/1‑250 (MQ=255)                                                                                                                                   aaTCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGc                                                                                    >  1:412038/1‑250 (MQ=255)                                                                                                                                            ggAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAAt                                                                          <  2:264869/251‑1 (MQ=255)                                                                                                                                                  ggTNAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTg                                                                    <  2:412038/251‑1 (MQ=255)                                                                                                                                                   gTGAAAGCCTCCAGCCGTCAGAACCAGATCATCCGCTTTGAACAGGATAAGAAACTGTTCCGTAACGAGCTGACAGTGCAAGGCTTGCCCGAAGGGTATGATAACGGTCCGCCGTTTAATAATCTCAACCTGCTGCTGGAAGTGGGGGAAAAACTGGCGGTCCTTGGGACAAACGGCCGCGGGCAATCAACCCAGCTGAAAACGCTGGTGGGCGACCTGCCACCGGACAGCGGCTCCGTAAAATGGTCTgt                                                                   >  2:147569/1‑250 (MQ=255)                                                                                                                                                             ccAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACgcgcgc                                                         <  1:6282/251‑1 (MQ=255)                                                                                                                                                             ccAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACgcgcgc                                                         >  2:124073/1‑251 (MQ=255)                                                                                                                                                               aGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGAGAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCAt                                                       <  1:452947/251‑1 (MQ=255)                                                                                                                                                                   gTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGt                                                   <  1:219834/251‑1 (MQ=255)                                                                                                                                                                        aaCCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTa                                              <  2:151102/251‑1 (MQ=255)                                                                                                                                                                         aCCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTAt                                             <  1:109017/251‑1 (MQ=255)                                                                                                                                                                                cATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTATGCTCAgg                                      <  2:446675/251‑1 (MQ=255)                                                                                                                                                                                      ttttGAACAGGATAAGTAACTGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACAAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGGAAATCAAAGCTGCTGAAAACGCTGGGGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCTTTGGTTACTATGCCCAGGACCACg                                >  1:391885/1‑251 (MQ=255)                                                                                                                                                                                                       aaCTGTTCCGTAACGCGCCGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTCCTGGGTACCAACGGCGTCGGTAAATCAACCCTGCTGTAAACGCTGGTGGGCGATATGCAACCGGACAGCGGCACCGGAAAATGGGCTGGGCACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAAt               >  2:433280/1‑251 (MQ=255)                                                                                                                                                                                                          tGTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACAGGCGGTACTGGGTACCAACGGCCTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTGCGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGAt            >  1:43989/1‑251 (MQ=255)                                                                                                                                                                                                           gTTCCGTAACGCGCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGAt            <  1:119845/250‑1 (MQ=255)                                                                                                                                                                                                                    cgcgCTGAAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCgtgt  <  1:134637/251‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    GTGAACGTCTGCTGGCCGATAACGCCAAGAAGAAAGCGCAGATTGCTGAGTTGCAATCTTTCGTTAGCCGCTTTAGCGCCAACGCCTCGAAATCTCGCCAGGCAACTTCGCGCGCGCGCCAGATTGATAAAATCAAACTGGAAGAGGTGAAAGCCTCCAGCCGTCAGAACCCGTTCATCCGTTTTGAACAGGATAAGAAACTGTTCCGTAACGCGCTGGAAGTGGAAGGTCTGACCAAAGGGTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTCTGAGAACGCGCGCATTGGTTACTATGCTCAGGACCACGAATATGAGTTTGAAAATGATCTGACCGTGT  >  NC_000913/856705‑857166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑