Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,015,156	G→A	L87L (TTG→TTA)	pqiC →	intermembrane transport lipoprotein PqiC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,015,156	0	G	A	100.0%	150.6 / NA	44	L87L (TTG→TTA)	pqiC	intermembrane transport lipoprotein PqiC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/22);  total (22/22)

ACGATTGCAGCACTGTGGCTGGCCGGATGCAGCTCCGGCGAAATTAATAAAAACTATTACCAGTTACCTGTGGTGCAGAGCGGTACACAAAGTACCGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATCAAACGTCCGTTCCGTCTGGAAGGAGTGCAAACTCAGGATGGTTACGATGAGATGGTT  >  NC_000913/1014914‑1015390                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           aCGATTGCAGCACTGTGGCTGGCCGGATGCAGCTCCGGCGAAATTAATAAAAACTATTACCAGTTACCTGTGGTGCAGAGCGGTACACAAAGTACCGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAacac                                                                                                                                                                                                                                    <  1:447624/251‑1 (MQ=255) aCGATTGCAGCACTGTGGCTGGCCGGATGCAGCTCCGGCGAAATTAATAAAAACTATTACCAGTTACCTGTGGTGCAGAGCGGTACACAAAGTACCGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAacac                                                                                                                                                                                                                                    >  1:3958/1‑251 (MQ=255)    aTTGCAGCACTGTGGCTGGCCGGATGCAGCTCCGGCGAAATTAATAAAAACTATTACCAGTTACCTGTGGTGCAGAGCGGTACACAAAGTACCGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCt                                                                                                                                                                                                                                 >  1:129297/1‑251 (MQ=255)              tgtgGCTGGCCGGATGCAGCTCCGGCGAAATTAATAAAAACTATTACCAGTTACCTGTGGTGCAGAGCGGTACACAAAGTACCGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAAc                                                                                                                                                                                                                       >  1:411703/1‑251 (MQ=255)                            tGCAGCTCCGGCGAATTTAATAAAAACTATTAACAGTTACCTGTGGTGCAGAGCGGTACACAAAGTACCGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACt                                                                                                                                                                                                         >  1:163215/1‑251 (MQ=255)                                  tCCGGCGAAATTAATAAAAACTATTACCAGTTACCTGTGGTGCAGAGCGGTACACAAAGTACCGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCgg                                                                                                                                                                                                   >  2:147021/1‑251 (MQ=255)                                            ttAATAAAAACTATTACCAGTTACCTGGGGTGCAGAGCGGGACACAAAGTACCGCAAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGGGAGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGAGGGCCAGACCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGGGTAAGCAACTGCACGGCTGGGGGGtt                                                                                                                                                                                         >  2:292084/1‑251 (MQ=255)                                                 aaaaaCTATTACCAGTTACCTGTGGTGCAGAGCGGTACACAAAGTACCGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTc                                                                                                                                                                                    <  2:282512/251‑1 (MQ=255)                                                    aaCTATTACCAGTTACCTGTGGTGCAGAGCGGTACACAAAGTACCGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTccc                                                                                                                                                                                  >  1:102100/1‑250 (MQ=255)                                                     aCTATTACCATTTCCCCGTGGTGCAGAACGGTACACAAAGTACCGCCAGCCATGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCGGGCGGGGAATGGTGTGGTTTAGCAAACCAGTGATGGGAAGTATGTGATTGCGAACAACAACTTGTTGGCCAGCCCTTTGGATCAACAGTTACGCAACACTCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAg                                                                                                                                                                                <  1:64442/251‑1 (MQ=255)                                                       tATTACCAGTTACCTGTGGTGCAGAGCGGTACACAAAGTACCGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAAATGCCCGGCTGGGTGGTTGCCTCCCAGcc                                                                                                                                                                              >  2:103050/1‑251 (MQ=255)                                                             cAGTTACCTGTGGTGCAGAGCGGTACACAAAGTACCGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTggg                                                                                                                                                                        >  1:130520/1‑251 (MQ=255)                                                                      gtggtgCAGAGCGGTACACAAAGTACCGCCCGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGccc                                                                                                                                                                >  1:398183/1‑250 (MQ=255)                                                                      gtggagCAGAGCGGTACAGAAAGTACCGCCGGCCAGGGCAATCGTCTGTTATGGGGAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGGCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCTGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCa                                                                                                                                                               <  2:4499/251‑1 (MQ=255)                                                                         gtgCAGAGCGGTACACAAAGTACCGCCAGCCAGGGCCATCGTCTGTTATGGGTAGAGCATGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCTAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGa                                                                                                                                                            <  2:153425/251‑1 (MQ=255)                                                                                     acacAAAGTACCGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGt                                                                                                                                                <  2:78132/251‑1 (MQ=255)                                                                                                cGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACgg                                                                                                                                     <  1:206503/251‑1 (MQ=255)                                                                                                  ccagccagGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATAGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTTGGCCAGCCCGTTGTATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGCGGTTGCCTTCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAg                                                                                                                                   <  1:292084/251‑1 (MQ=255)                                                                                                                              agagCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTc                                                                                                        <  1:22645/250‑1 (MQ=255)                                                                                                                               gagCAGGTCACTGTTACTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAACCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGCTCGCTATGATGGCAAGGTCAt                                                                                                      >  2:218585/1‑251 (MQ=255)                                                                                                                                  cAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACTCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGt                                                                                                   <  1:178584/251‑1 (MQ=255)                                                                                                                                     gTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCaa                                                                                                                                                   <  1:239614/200‑1 (MQ=255)                                                                                                                                     gTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCaa                                                                                                                                                   >  2:239614/1‑200 (MQ=255)                                                                                                                                       cACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTg                                                                                                                                                                                        >  1:307064/1‑161 (MQ=255)                                                                                                                                       cACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTg                                                                                                                                                                                        <  2:307064/161‑1 (MQ=255)                                                                                                                                          tGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTAAGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGGCAgtggtg                                                                                           >  2:307253/1‑251 (MQ=255)                                                                                                                                                              ggAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCCCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAaccacc                                                                         >  1:266817/1‑249 (MQ=255)                                                                                                                                                                            tATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACATCTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATCa                                                          <  2:342772/250‑1 (MQ=255)                                                                                                                                                                            caTCAAACCAGTGATGTGAATTGAGTGATTGCCAACAACAGGTTGTGGGACACCCCGTTGGATCAACAGTGACGCAAATCCCTGGTTGCCAACCTTAGTACGCAACTTCCCGGCTGGGTGGTTGCCTGCCAGCATCTGGTACGCTCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGGCATTGTCAGTGGGGAGTGGCGGTTGAgccactgggtattactcatca                                                          <  2:261845/249‑17 (MQ=255)                                                                                                                                                                                aaaCCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATCAAACGt                                                     >  1:402259/1‑251 (MQ=255)                                                                                                                                                                                aaaCCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATCAAACGt                                                     >  2:183659/1‑251 (MQ=255)                                                                                                                                                                                 aaCCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGagatcggaag                                                    <  1:103050/251‑10 (MQ=255)                                                                                                                                                                                         aTGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTa                                                                                                                     >  1:438917/1‑178 (MQ=255)                                                                                                                                                                                         aTGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTa                                                                                                                     <  2:438917/178‑1 (MQ=255)                                                                                                                                                                                           gtgAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATCAAACGTCCGTTCCGTCt                                          >  1:139081/1‑251 (MQ=255)                                                                                                                                                                                                gTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATCAAACGTCCGTTCCGTCTGGAAg                                     <  2:129297/251‑1 (MQ=255)                                                                                                                                                                                                  aTGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGATCCACCAGGGTCAACTGATCAAACGTCCGTTCCGTCTGGAAGGa                                   <  1:377339/251‑1 (MQ=255)                                                                                                                                                                                                             aacaacaacTTGTGGGACAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATCAAACGTCCGTTCCGTCTGGAAGGAGTGCAAACTc                         >  1:405166/1‑250 (MQ=255)                                                                                                                                                                                                                       tntgGGCCAGCCAGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATCAAACGTCCGTTCCGTCTGGAAGGAGTGCAAACTCAGGATGGtt                <  2:417340/249‑1 (MQ=255)                                                                                                                                                                                                                       tgtgGGCCAGCCCGTTGGATCAACAGTTACGCAACGCCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTGTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATAAAACGTCCGTTCCGTCTGGAAGGAGTGCAAACTCAGGATGGTTa               <  2:23272/250‑1 (MQ=255)                                                                                                                                                                                                                        gtgGGCCAGCCCGTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATCAAACGTCCGTTCCGTCTGGAAGGAGTGCAAACTCAGGATGGTTAc              <  1:201079/250‑1 (MQ=255)                                                                                                                                                                                                                           ggCCAGCCCGTTGCATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCGACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATCAAACGTCCGTTCCGTCTGGAAGGAGTGCAAACTCAGGATGGTTACGATg          <  1:165525/251‑1 (MQ=255)                                                                                                                                                                                                                                    gTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATCAAACGTCCGTTCCGTCTGGAAGGAGTGCAAACTCAGGATGGTTACGATGAGATGGtt  >  1:246299/1‑250 (MQ=255)                                                                                                                                                                                                                                    gTTGGATCAACAGTTACGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATCAAACGTCCGTTCCGTCTGGAAGGAGTGCAAACTCAGGATGGTTACGATGAGATGGtt  >  1:298727/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           ACGATTGCAGCACTGTGGCTGGCCGGATGCAGCTCCGGCGAAATTAATAAAAACTATTACCAGTTACCTGTGGTGCAGAGCGGTACACAAAGTACCGCCAGCCAGGGCAATCGTCTGTTATGGGTAGAGCAGGTCACTGTTCCTGACTATCTGGCGGGGAATGGTGTGGTTTATCAAACCAGTGATGTGAAGTATGTGATTGCCAACAACAACTTGTGGGCCAGCCCGTTGGATCAACAGTTGCGCAACACCCTGGTTGCCAACCTGAGTACGCAACTGCCCGGCTGGGTGGTTGCCTCCCAGCCTCTGGGAAGCGCCCAGGACACGCTCAATGTTACCGTAACGGAGTTTAACGGTCGCTATGATGGCAAGGTCATTGTCAGTGGTGAGTGGCTGTTGAACCACCAGGGACAACTGATCAAACGTCCGTTCCGTCTGGAAGGAGTGCAAACTCAGGATGGTTACGATGAGATGGTT  >  NC_000913/1014914‑1015390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑