Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	463,581	G→A	S556N (AGC→AAC)	ppiD →	periplasmic folding chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	463,581	0	G	A	100.0%	149.7 / NA	44	S556N (AGC→AAC)	ppiD	periplasmic folding chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/23);  total (21/23)

GCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAGCTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAAC  >  NC_000913/463337‑463827                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       gCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACgg                                                                                                                                                                                                                                                  <  2:349331/251‑1 (MQ=255)        aaCCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGGTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCg                                                                                                                                                                                                                                           <  2:54694/251‑1 (MQ=255)                   gATGTTCAGGAACAAGTTAAGGGATGGTTTCAGCACAACAAAGCTGAACAACATGCGAAAGTGGATGCTGAGAAACTGCTGGTTGATGTGAAAGCCGTCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAACCCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGGCAAACCGAACTACGGTATGGCGACCGATATGCa                                                                                                                                                                                                                                <  2:315764/251‑1 (MQ=255)                              aCAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGGGCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCTACTTCCAGCGAAAGGCAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTgg                                                                                                                                                                                                                     <  1:44263/251‑1 (MQ=255)                                 aGTTAAGGCATTGGATCAGCACAAAAAAGCTGATCAACAGGCGATAGTGCATGCTGAGAAACTGCTGGCTGATTTGAAAGCCCGCAAAGGTGCGGAAGCTATGCAGGCTGACGGTCTGAAATTTCGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGCGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCCACAGATATCCAAGGTAAtgcggcgc                                                                                                                                                                                                                  >  2:431563/1‑245 (MQ=255)                                        gcactGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGgc                                                                                                                                                                                                           <  1:307938/247‑1 (MQ=255)                                                            aGCTGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGTTGATTTGAAAGCCGGCACAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCAATGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACa                                                                                                                                                                                         >  1:281792/1‑249 (MQ=255)                                                               tGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGtt                                                                                                                                                                                    >  1:123108/1‑251 (MQ=255)                                                                                              ctgctgGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGAGGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATggg                                                                                                                                                     >  2:45558/1‑250 (MQ=255)                                                                                                gctgGTTGATTTGAAAGCCGGCAAAGGTGTGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACCACCCGAACTACGGTATGGCGACCGATATGCAAGGTCATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGc                                                                                                                                                   >  1:196359/1‑251 (MQ=255)                                                                                                gctgGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTTAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGt                                                                                                                                                     <  1:235494/249‑1 (MQ=255)                                                                                                           tGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTGAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGTAATATCAGAAAAAAGCGATGGTGCAGGGTATCAc                                                                                                                                         <  1:242059/250‑1 (MQ=255)                                                                                                                 ccGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGaac                                                                                                                                  <  1:444127/251‑1 (MQ=255)                                                                                                                 ccGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGaac                                                                                                                                  >  2:351015/1‑251 (MQ=255)                                                                                                                              ggAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCg                                                                                                                     >  2:309979/1‑251 (MQ=255)                                                                                                                              ggAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCAAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCg                                                                                                                     >  1:261831/1‑251 (MQ=255)                                                                                                                                aaGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTc                                                                                                                   >  1:113619/1‑251 (MQ=255)                                                                                                                                aaGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGACGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTGCGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCTCCCGGAACAACGCAAAAATCGTc                                                                                                                   <  2:271322/251‑1 (MQ=255)                                                                                                                                                 ggTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCtgatg                                                                                                    >  2:247491/1‑249 (MQ=255)                                                                                                                                                      gAAATTTGGCGAGCCGAAAACCTTAAGCCGGTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTa                                                                                                <  2:293365/248‑1 (MQ=255)                                                                                                                                                       aaaTTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGCAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCt                                                                                            >  2:81088/1‑251 (MQ=255)                                                                                                                                                         aTTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGc                                                                                          <  1:269448/251‑1 (MQ=255)                                                                                                                                                                gAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGAGTaatg                                                                                    >  2:163700/1‑248 (MQ=255)                                                                                                                                                                     gAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCg                                                                               >  1:392721/1‑250 (MQ=255)                                                                                                                                                                             tAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTTCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCaa                                                                       <  1:260019/250‑1 (MQ=255)                                                                                                                                                                             tAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCa                                                                        <  1:26220/249‑1 (MQ=255)                                                                                                                                                                               aGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAAt                                                                    <  1:167230/251‑1 (MQ=255)                                                                                                                                                                                gCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAACTCAAAAtt                                                                   >  1:43123/1‑251 (MQ=255)                                                                                                                                                                                       cGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATg                                                            >  2:161321/1‑251 (MQ=255)                                                                                                                                                                                        ggTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATgc                                                           >  1:84752/1‑251 (MQ=255)                                                                                                                                                                                        ggTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTATGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATgc                                                           <  1:64556/251‑1 (MQ=255)                                                                                                                                                                                           cGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCt                                                        <  1:431258/251‑1 (MQ=255)                                                                                                                                                                                              gACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTgg                                                      >  1:33525/1‑250 (MQ=255)                                                                                                                                                                                              gACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGa                                                     <  1:319985/251‑1 (MQ=255)                                                                                                                                                                                                   gATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAAGGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGc                                                <  2:82848/251‑1 (MQ=255)                                                                                                                                                                                                          cAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAACTCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATc                                         >  2:16837/1‑251 (MQ=255)                                                                                                                                                                                                           aGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATcc                                        <  1:247491/251‑1 (MQ=255)                                                                                                                                                                                                               ggcgTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGaa                                    <  2:281792/251‑1 (MQ=255)                                                                                                                                                                                                                       cactgcaactgcCAGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACACGGATCAATGCCGGAAGATCAGAACAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTc                            >  2:178690/1‑251 (MQ=255)                                                                                                                                                                                                                                    aGCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCg               >  1:298716/1‑251 (MQ=255)                                                                                                                                                                                                                                     gCGAAAGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGt              >  2:455749/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCTGCCTCGCAAAAAAATGCGTTGCa          <  2:213770/251‑1 (MQ=255)                                                                                                                                                                                                                                         aaGACAAACCGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCa          <  1:455749/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ccGAACTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGGTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAAc  <  2:33525/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GCGGTGAAACCGTTGGCAGATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAAGTGGATGCTGAGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATTAGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAGCTACGGTATGGCGACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATGCCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTCTTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTGGAACAGCAATAATCCTGAAGCCGCCTCGCAAAAAAATGCGTTGCAGCTGTAAC  >  NC_000913/463337‑463827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑