Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	469,435	G→A	A189T (GCA→ACA)	mdlA →	ABC transporter family protein MdlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	469,435	0	G	A	100.0%	187.9 / NA	57	A189T (GCA→ACA)	mdlA	ABC transporter family protein MdlA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/30);  total (27/30)

CATGGCTCGTGCGACCAATGACGTCGATCGC‑GTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGC  >  NC_000913/469218‑469680                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      catagCTCGTGCGACCAATGACGTCGATCGC‑GTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCa                                                                                                                                                                                                                      <  1:360324/247‑1 (MQ=255)    ggCTCGTGCGTCCTATGACGTCGATCGCAGT‑GTTGTTGCCGCCGGAGAAGGGGTTCTTACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGGTGATTATGATTTCTACGCAAATTGGCTGACAGTTGACATTATTTTCCCTTTTGCCAATGGCAGTGATGACGAT‑CATGATTAAGCGTAACAGCGATGTTTGGCATGAACGCTTTAAGCTTACACAGGCTGTGTTTTCTAGTCTTAATGACCGCAccc                                                                                                                                                                                                                   <  2:435958/251‑1 (MQ=255)    ggCTCGTGCGACCAATGACGTCGATCGC‑GTCGTGTTTGCCGCCGGATAATGGGTGCTTACGCTGGTGTATTCACTGGTGATCGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTATCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCAcc                                                                                                                                                                                                                    <  1:224306/250‑1 (MQ=255)     gCTCGTGCGACCAATGACGTCGATCGC‑GTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCa                                                                                                                                                                                                                  <  1:125591/251‑1 (MQ=255)     gCTCGTGCGACCAATGACGTCGATCGC‑GTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCa                                                                                                                                                                                                                  <  1:92809/251‑1 (MQ=255)        ggTGCGACCAATGACGTCGATCGC‑GTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGa                                                                                                                                                                                                               <  2:308060/250‑1 (MQ=255)             gACCAATGACGTCGATCGC‑GTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAATGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGcc                                                                                                                                                                                                          >  1:310550/1‑251 (MQ=255)                   tGACGTCGATCGC‑GTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCAcc                                                                                                                                                                                                     >  1:217739/1‑250 (MQ=255)                   ggACGTCGATCGC‑GTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAATCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACGCAGGAAAGCCTCACCa                                                                                                                                                                                                    <  2:269501/250‑1 (MQ=255)                                         tGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCt                                                                                                                                                                               >  2:207417/1‑251 (MQ=255)                                             gccgGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTgg                                                                                                                                                                           >  2:321814/1‑251 (MQ=255)                                                      ggggTGCTGACGCTGGTGGATTCCCTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGa                                                                                                                                                                     >  2:170059/1‑248 (MQ=255)                                                       gggTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGAt                                                                                                                                                                 <  1:166936/251‑1 (MQ=255)                                                        ggTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATc                                                                                                                                                                <  2:396325/251‑1 (MQ=255)                                                           gCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGcc                                                                                                                                                             >  1:407414/1‑251 (MQ=255)                                                           gCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGc                                                                                                                                                              <  1:431296/250‑1 (MQ=255)                                                              gACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGGGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCGGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCTCCAGTATCCGCATGATCAAAGCCGTTGGTCTGGAAGATCGCCAg                                                                                                                                                           <  2:30933/250‑1 (MQ=255)                                                                    ggtggATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCg                                                                                                                                                     <  2:374281/250‑1 (MQ=255)                                                                       ggATTCACTGGTGAAGGGCTGCGCTGTGTTGATTATGATGACTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTAt                                                                                                                                                 >  2:134159/1‑251 (MQ=255)                                                                            cACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGcc                                                                                                                                            >  2:199212/1‑251 (MQ=255)                                                                                   gATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGAt                                                                                                                                      <  1:62823/250‑1 (MQ=255)                                                                                       ggCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGa                                                                                                                                 <  1:110904/251‑1 (MQ=255)                                                                                         cTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGa                                                                                                                                 >  1:48445/1‑249 (MQ=255)                                                                                                         atgatgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCaaaaaaa                                                                                                                >  2:197927/1‑250 (MQ=255)                                                                                                                 tACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGaa                                                                                                                                <  2:220166/226‑1 (MQ=255)                                                                                                                 tACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGaa                                                                                                                                >  1:220166/1‑226 (MQ=255)                                                                                                                  aCGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGCCGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCa                                                                                                                                                            >  1:52813/1‑197 (MQ=255)                                                                                                                  aCGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCa                                                                                                                                                            <  2:52813/197‑1 (MQ=255)                                                                                                                         ttAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGt                                                                                               >  2:353207/1‑251 (MQ=255)                                                                                                                          caGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGt                                                                                               >  1:162049/2‑250 (MQ=255)                                                                                                                              tGGCAGTTGACCTTATTTTGACTGTTGCCGCTGCCAGCGATGAC‑ATACATGATTAAGCGCACCGGCGATGCTTTGCATGAGCGCTTTAATCTGACACAGGCGGCGTTTTCCCGTCTTAGTGACCGTACCCAGCGCAGCCTCACCAGTCTCCGCATGTTTTAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATTCCGAAGATCCCAGCAAATATAAAATGGGGATGGCGCGTAttt                                                                                           >  2:209655/1‑249 (MQ=255)                                                                                                                                   gTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTc                                                                                     <  2:61014/251‑1 (MQ=255)                                                                                                                                           tATTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTcg                                                                               <  1:333143/249‑1 (MQ=255)                                                                                                                                            aTTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGtt                                                                                                                                                   >  1:60342/1‑180 (MQ=255)                                                                                                                                            aTTTTCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGtt                                                                                                                                                   <  2:60342/180‑1 (MQ=255)                                                                                                                                             ttttCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGc                                                                                       >  1:183796/1‑239 (MQ=255)                                                                                                                                             ttttCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGGTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCTGGTGGCGCGTATTGATGc                                                                                       <  2:183796/239‑1 (MQ=255)                                                                                                                                              tttCCCTGTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGTAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATGCCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTcgaccc                                                                           <  1:57435/250‑1 (MQ=255)                                                                                                                                                     gTTGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCAt                                                                     <  1:240909/249‑1 (MQ=255)                                                                                                                                                      ttGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCt                                                                   >  1:382612/1‑250 (MQ=255)                                                                                                                                                      ttGCCGATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACAGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAACACATGCGGGGGGAGCGTATTGATGCTCGTTTCGACCCGACCATCt                                                                   >  2:183436/1‑250 (MQ=255)                                                                                                                                                           gATGCCAGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATcg                                                             <  1:96310/251‑1 (MQ=255)                                                                                                                                                                 aGTGATGACGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCATTTCGACCCGACCATCTATATCGCGATTg                                                       <  1:386001/251‑1 (MQ=255)                                                                                                                                                                     atgacGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTAt                                                   <  1:199212/247‑1 (MQ=255)                                                                                                                                                                     atgacGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTAt                                                   >  1:148140/5‑251 (MQ=255)                                                                                                                                                                      tgacGAT‑CATGATTAAGCGCAACGGCGCTGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATg                                                  >  1:117541/4‑251 (MQ=255)                                                                                                                                                                          gAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGTGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATggaga                                              <  1:64239/251‑4 (MQ=255)                                                                                                                                                                           aT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGaa                                             <  1:207417/251‑1 (MQ=255)                                                                                                                                                                                  aTTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAACAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGAGAACTTGCt                                       >  2:79099/1‑251 (MQ=255)                                                                                                                                                                                            aCGGCGATGCTTTGCATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTggc                             >  1:256767/1‑251 (MQ=255)                                                                                                                                                                                                    gCTTTGCATTAACGCTTTAAGCTGACACAGGCTGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCCCCAGTATCCGCATGACCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAg                     <  1:228053/251‑1 (MQ=255)                                                                                                                                                                                                       ttGCATGAACGCGTTAAGCTTACTCAGGCGGCGTGTTCCAGTCTTAATGACCGCACCCAGGAAAGCCGCACCAGTATCCGCATGATCAAATCATCTGGTCTGGAAGATCGCCAGTCGGCGTGATTTGCCGCGGATGACGAAGATACCGGCAAAAAAAACAGGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGTCGATTGGCGGCGGTAGtt                   <  2:241445/250‑1 (MQ=255)                                                                                                                                                                                                          cATGAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGGGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGCAGGTGGAt               >  1:97896/1‑251 (MQ=255)                                                                                                                                                                                                             gAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGAtggg            >  1:299523/1‑250 (MQ=255)                                                                                                                                                                                                             gAACGCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCCATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGtctttcgctggg            >  2:184446/1‑240 (MQ=255)                                                                                                                                                                                                                 gCTTTAAGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGgtggtg        <  2:117541/251‑1 (MQ=255)                                                                                                                                                                                                                       aGCTGACACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGCTGGGGGTGCAGGGc  >  1:246118/1‑251 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      CATGGCTCGTGCGACCAATGACGTCGATCGC‑GTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGAT‑CATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGC  >  NC_000913/469218‑469680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑