Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	383,203	A→G	W245R (TGG→CGG)	yaiP ←	putative glucosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	383,203	0	A	G	100.0%	193.1 / NA	58	W245R (TGG→CGG)	yaiP	putative glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/27);  total (31/27)

GCTAAAAGCACCAATAACACAAACTACGCCGACCCAGATAAGCGGAAACATTGCCAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACG  >  NC_000913/382961‑383447                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     ccTAAAAGCACCAATAACACAACCTACGCCGACCCAGATAAGCGGAAACATTGCCAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTTAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACTACCAACAGCATATGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATCCAGACCGCGTATCCCACAATCCAACGCCGCCAGCGAAGCCGTTCCTCAc                                                                                                                                                                                                                                              <  2:354962/250‑1 (MQ=255)                aaCACAAACTACGCCGACCCAGATAAGCGGAAACATTGCCAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCAtt                                                                                                                                                                                                                               >  1:343559/1‑251 (MQ=255)                           cGCCGACCCAGATAAGCGGAAACATTGCCAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGACGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGtat                                                                                                                                                                                                                    >  1:48345/1‑251 (MQ=255)                                aCCCAGATAAGCGGAAACATTGCCAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGAt                                                                                                                                                                                                               <  1:178965/251‑1 (MQ=255)                                         aGCGGAAACATTGCCAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGAtt                                                                                                                                                                                                      >  1:161117/1‑251 (MQ=255)                                            ggAAACATTGCCAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCACCGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGACGCGGCTAAATAAAAGTCTTTTATGCAGGAGAATACAGACCGCGTATCCCCCACTCCAACGCCGACAGCGCCGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGAAGCGATTCGc                                                                                                                                                                                                   >  2:168643/1‑251 (MQ=255)                                            ggAAACATTGCCAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAACTATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGCCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCATGTGGGTATACGATGCAGCGATTCGc                                                                                                                                                                                                   >  1:261233/1‑251 (MQ=255)                                             gaaccATTACCAACACCCCTCCATGCGACCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCCCAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGCCTTTTATGCCGGCGCATACAGACCGCGTAGCCCACAATCCAACGGCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTTCATTCCTGTGGGTATACGATGCAGCGATTCGcc                                                                                                                                                                                                  <  2:455696/247‑1 (MQ=255)                                                 cATTGCCAAAACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACGGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGcc                                                                                                                                                                                              <  2:285084/251‑1 (MQ=255)                                                  aTTGCCAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCg                                                                                                                                                                                             >  2:391459/1‑251 (MQ=255)                                                      ccAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGACAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGAATACGATGCAGCGATTCGCCTGCCGAAt                                                                                                                                                                                          >  2:61020/1‑250 (MQ=255)                                                       cAACCCCACTCCATGCGGCCCGTTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGGCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGTCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATAc                                                                                                                                                                                        <  1:61020/251‑1 (MQ=255)                                                                       ggCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGccac                                                                                                                                                                        >  2:65766/1‑251 (MQ=255)                                                                            ggtggtGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATg                                                                                                                                                                   <  1:452184/251‑1 (MQ=255)                                                                                  gatgaATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAgg                                                                                                                                                             >  1:146858/1‑251 (MQ=255)                                                                                  gatgaATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAgg                                                                                                                                                             >  2:348818/1‑251 (MQ=255)                                                                                      aaTTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTgag                                                                                                                                                         <  2:447232/251‑1 (MQ=255)                                                                                         tCATTAAACCAGGTAGTGATATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCTAAGCGGCTAAATAAAAGTCTTGTATGCAGGCGCATACAGACCGCGTATCCCACAATCCATCTCCGCCAGCGACGCCGCTCCTCGCGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGTTGAGAt                                                                                                                                                       <  2:261233/250‑1 (MQ=255)                                                                                           aTTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGAACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGAAGCCGTTTGCCACCAATGTCCAGGTGAGATCa                                                                                                                                                     >  1:259513/1‑250 (MQ=255)                                                                                                               aaaTCCCAACGACATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACTCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTaag                                                                                                                                 <  2:334958/250‑3 (MQ=255)                                                                                                                 aTCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATc                                                                                                                              <  1:65766/251‑1 (MQ=255)                                                                                                                  tCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATcc                                                                                                                             >  1:51473/1‑251 (MQ=255)                                                                                                                   cccAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCg                                                                                                                            <  2:51473/251‑1 (MQ=255)                                                                                                                    ccAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCg                                                                                                                            >  2:57177/1‑250 (MQ=255)                                                                                                                           cATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCg                                                                                                                    >  1:328522/1‑251 (MQ=255)                                                                                                                                          acAGCATAGGAGATATACTGAAGATAACGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCGTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCCATAc                                                                                                      >  2:167453/1‑250 (MQ=255)                                                                                                                                                aaGNAAATATACGGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGTTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAGACCGAACTTACGCAATACATCAGTa                                                                                               <  2:410842/250‑1 (MQ=255)                                                                                                                                                 aGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTAc                                                                                              >  1:187554/1‑251 (MQ=255)                                                                                                                                                      agataCTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAAc                                                                                         <  2:343559/249‑1 (MQ=255)                                                                                                                                                                      gAAGCGGCTACATAAAAGTCTTGTATGCAGGCGCATACAGACCTCGTCTCCCACAATCCAACGCCGCCAGCTACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGc                                                                         <  2:386738/251‑1 (MQ=255)                                                                                                                                                                        aGCGGCTAAATAAAAGTCTTTTATGCAGGCGCTTACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGAGCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTg                                                                       <  2:179035/251‑1 (MQ=255)                                                                                                                                                                           ggCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGat                                                                     >  1:446904/1‑250 (MQ=255)                                                                                                                                                                               aaataaaAGTCTTTTATGCAGTCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGAtaataa                                                                <  1:63408/251‑1 (MQ=255)                                                                                                                                                                               aaataaaAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGataata                                                                 >  2:101045/1‑250 (MQ=255)                                                                                                                                                                                 ataaaAGACTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACACCGCCAGCGACGCCGCTCCTCACGCGGCCTGCTGCATTCCTGTGGGCATACGATGCAGCGATTCGCCTGCCGAATCCGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTCGTACGATCCGCGAAACCGAACTTACGCAATACATCAGTACGGACCATCCCGCAGGCACCGCTGATAATAAAc                                                              >  2:54539/1‑251 (MQ=255)                                                                                                                                                                                    aaaGGCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGt                                                           >  1:259450/1‑251 (MQ=255)                                                                                                                                                                                     aaGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTg                                                          >  1:74392/1‑251 (MQ=255)                                                                                                                                                                                      aGTCTTTTATGAAGGCGCATACCGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTgc                                                         >  2:266048/1‑251 (MQ=255)                                                                                                                                                                                           ttttggCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTGTTCGACTGTAGTACGTCCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATATACGGCGCGCCa                                                     <  2:32185/245‑1 (MQ=255)                                                                                                                                                                                              aTGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCAccc                                                  >  1:130981/1‑250 (MQ=255)                                                                                                                                                                                              aTGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCAccc                                                  <  2:161117/250‑1 (MQ=255)                                                                                                                                                                                                 cAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACTGTGCGCCACCCAGGa                                              <  1:169238/251‑1 (MQ=255)                                                                                                                                                                                                        aTACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGctgctg                                       >  2:447330/1‑251 (MQ=255)                                                                                                                                                                                                          aCAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTCGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGt                                      >  2:293843/1‑250 (MQ=255)                                                                                                                                                                                                           cagcccGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAg                                    <  1:181756/247‑1 (MQ=255)                                                                                                                                                                                                             gaccgcgaataccaccatccctcaccgccAGCGACGCCGCTACTCACCCGGGCTGTTGCATTCCTGCAGGCATACGATGCACCGATTCGCCTGCCGAATACAGTAGACGTTTGCCACCACTATCCACGGGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCACTACATCAGTACGGAACATCACGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCg                                  >  2:186463/25‑251 (MQ=255)                                                                                                                                                                                                             gACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCg                                  >  1:359416/1‑251 (MQ=255)                                                                                                                                                                                                                     aTCCCACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTc                          <  1:391459/251‑1 (MQ=255)                                                                                                                                                                                                                       cccACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGTGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCAt                        <  2:65176/251‑1 (MQ=255)                                                                                                                                                                                                                       cccACAATCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTTTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGTCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCa                         <  1:36403/250‑1 (MQ=255)                                                                                                                                                                                                                             aTCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAat                  <  2:118784/251‑1 (MQ=255)                                                                                                                                                                                                                              tCCAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAatc                 >  1:211682/1‑251 (MQ=255)                                                                                                                                                                                                                               ccAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAatc                 >  1:232446/1‑250 (MQ=255)                                                                                                                                                                                                                                cAACGCCGCCAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAatcat               <  1:101045/251‑1 (MQ=255)                                                                                                                                                                                                                                        ccAGCGACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACt       <  1:197018/251‑1 (MQ=255)                                                                                                                                                                                                                                             gACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAAt                                                                                                        <  1:197029/149‑1 (MQ=255)                                                                                                                                                                                                                                             gACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAAt                                                                                                        >  2:197029/1‑149 (MQ=255)                                                                                                                                                                                                                                             gACGCCGCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACg  >  2:27589/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     GCTAAAAGCACCAATAACACAAACTACGCCGACCCAGATAAGCGGAAACATTGCCAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGCCACCCAGGAGCTGCTGTAGCGTGCGCTTCATAACAATCATCGGCAACTTTACG  >  NC_000913/382961‑383447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑