Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	769,601	G→A	V542I (GTA→ATA)	mngB →	alpha‑mannosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	769,601	0	G	A	100.0%	93.6 / NA	29	V542I (GTA→ATA)	mngB	alpha‑mannosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/15);  total (14/15)

GATTGTCCCTTCTATGGGCTATCGCACGCTTTATATCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGGTAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGT  >  NC_000913/769354‑769839                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               gATTGTCCCTTCTATGGGCTATCGCACGCTTTATATCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATaa                                                                                                                                                                                                                                             <  2:330950/251‑1 (MQ=255)                gggCTATCGCACGCTTTATATCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAAcg                                                                                                                                                                                                                              <  2:22954/251‑1 (MQ=255)                  gCTATCGCACGCTTTATATCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTAc                                                                                                                                                                                                                                         >  1:170768/1‑238 (MQ=255)                  gCTATCGCACGCTTTATATCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTAc                                                                                                                                                                                                                                         <  2:170768/238‑1 (MQ=255)                           cGCTTTATATCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGaa                                                                                                                                                                                                                          >  1:268049/1‑244 (MQ=255)                           cGCTTTATATCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGaa                                                                                                                                                                                                                          <  2:268049/244‑1 (MQ=255)                               ttATATCGCAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGAATAATTACCGCAGCGAACGCGAAACAGCAATGCg                                                                                                                                                                                                               >  2:273580/1‑251 (MQ=255)                                 atatCGAAGCGAATCAGCCGGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGat                                                                                                                                                                                                             <  2:233645/251‑1 (MQ=255)                                   atCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGatat                                                                                                                                                                                                           >  1:247211/1‑251 (MQ=255)                                     cgtaGCGAATCAGCGTGGCAACGTAATTGAGGCAATAAGCGACGCTGAAGGGCTACTGGACAATGCTTTCTGGCAAATGGCGCTCAATGCGGATGGGTATCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATGCACCCGCAATAGAAGAGTGGAAAATTATCGCAGCGAATGCGAAACCGCAAGACGAtatt                                                                                                                                                                                                          >  2:280149/4‑250 (MQ=255)                                                                             gACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGGCAGCGTTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGGTGAATATGTCTAGTCACCCGCAAAAGAAGAGTGGATAATGTCCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATg                                                                                                                                                                  <  1:203647/250‑1 (MQ=255)                                                                                      gggATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAgg                                                                                                                                                        >  2:391842/1‑250 (MQ=255)                                                                                                   aTGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTCTGACATGGCAGTGCCGCTCAANTTg                                                                                                                                           >  2:417702/1‑251 (MQ=255)                                                                                                            ggCAAATTGCGCTCAATGAGGATGGTTCTCTGGAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTGTGATGATGGTGATGAATATGACTATGCCCCCGCAAAAGAAGAGGGGATAAATACCGCAACGAACGCGAAATCACAATGCGATATTATTCATGAAGCCGGGCAGAGCAGGGCTGTTATCCGCTATGACATTGCAGTGCCGGTCAATTAGTCAGAACGc                                                                                                                                  <  2:283491/251‑1 (MQ=255)                                                                                                                 aTTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAgcgc                                                                                                                             >  1:331390/1‑251 (MQ=255)                                                                                                                   tGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGcc                                                                                                                            <  1:324265/250‑1 (MQ=255)                                                                                                                    gcgcTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGccc                                                                                                                           <  1:19256/250‑1 (MQ=255)                                                                                                                         cAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAAt                                                                                                                     <  2:1512/251‑1 (MQ=255)                                                                                                                              aGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACt                                                                                                                >  1:447765/1‑251 (MQ=255)                                                                                                                                gATGGTTCTCTGCAACTGGTAGATAAAGACAACGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTgg                                                                                                              >  1:399987/1‑251 (MQ=255)                                                                                                                                         ctGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTa                                                                                                       <  1:244296/249‑1 (MQ=255)                                                                                                                                                       tAAAGACAGCGGTGAGCGCGATGACCGGGGAGTGCAGGTTGAAGAAAGCTCTGATGATGGTGATGAAAATGAATATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAAGGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGAAATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGACAGGCAATCCACTGACAGAGTAGGGGAGGTgttggttg                                                                                       >  1:142210/1‑251 (MQ=255)                                                                                                                                                                         cTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAAc                                                                      >  1:397327/1‑250 (MQ=255)                                                                                                                                                                           aTGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTAGTTGTCACTCTTAGTCATAAcagc                                                                   <  2:402479/251‑1 (MQ=255)                                                                                                                                                                                cGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTAGTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCg                                                              <  2:260257/251‑1 (MQ=255)                                                                                                                                                                                    tATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGACAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTAtt                                                          >  1:292542/1‑251 (MQ=255)                                                                                                                                                                                                  gaaAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCa                                             >  2:161913/1‑250 (MQ=255)                                                                                                                                                                                                                                        cGCAAAAGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTcc      <  1:417702/251‑1 (MQ=255)                                                                                                                                                                                                                                            aaaGAAGAGTGGATAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGt  <  1:161306/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               GATTGTCCCTTCTATGGGCTATCGCACGCTTTATATCGAAGCGAATCAGCCTGGCAACGTAATTGCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAATGAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTGCAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAGTGGGTAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAGAGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCCCGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGGCGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTGGT  >  NC_000913/769354‑769839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑