Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,079,999	G→A	G232D (GGT→GAT)	putP →	proline:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,079,999	0	G	A	100.0%	105.5 / NA	32	G232D (GGT→GAT)	putP	proline:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/19);  total (13/19)

TGAAAGTACCTTTGGCATGAGCTACGAAACGGCTCTGTGGGCGGGCGCTGCGGCGACGATCCTTTACACCTTTATTGGCGGTTTCCTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTGTCGGCTTCTTTGGGATTGCTTACTTTAACGATCATCCGGCGTTGGCTGGTGCGGTAAATCAGAACGCCGAGC  >  NC_000913/1079760‑1080238                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                ggaaaggaccgggggCATGAGCTACGAAACGGCTCTGTGGGCGGGCGCTGCGGCGACGATCCTTTACACCTTTATTGTCGGTTTCCTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCTCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTttg                                                                                                                                                                                                                                      <  1:27191/238‑1 (MQ=255)  gaaaggtccTTTGGCATGAGCTGCGACACGGCTCTGTGGGCGGGCGCTGCTGCGACGATCCTTTACACCTTTATTTGCGGTTTCCTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATGTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTttgt                                                                                                                                                                                                                                     <  2:245165/244‑1 (MQ=255)  gAAAGTACCTTTGGCATGAGCTACGAAACGGCTCTGTGGGCGGGCGCTGCGGCGACGATCCTTTACACCTTTATTGGCGGTTTCCTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTttgt                                                                                                                                                                                                                                     <  1:201089/251‑1 (MQ=255)        aCCTTTGGCATGAGCTACGAAACGGCTCTGTGGGCGGGCGCTGCGGCGACGATCCTTTACACCTTTATTGGCGGTTTCCTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCAt                                                                                                                                                                                                                               <  1:71983/251‑1 (MQ=255)                   gAGCTACGAAACGGCTCTGTGGGCGGGCGCTGCGGCGACGATCCTTTACACCTTTATTGGCGGTTTCCTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTg                                                                                                                                                                                                                     <  1:7001/250‑1 (MQ=255)                   gAGCTACGAAACGGCTCTGTGGGCGGGCGCTGCGGCGACGATCCTTTACACCTTTATTGGCGGTTTCCTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTg                                                                                                                                                                                                                     <  1:380778/250‑1 (MQ=255)                        aCGAAACGGCTCTGTGGGCGGTCGCTGCGGCGACGATCCTTTACACCTTTATTGGCGGTTTCCTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGt                                                                                                                                                                                                               <  2:246300/251‑1 (MQ=255)                         cGAAACGGCTCTGTGGGCGGGCGCTGCGGCGACGATCCTTTACACCTTTATTGGCGGTTTCCTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGAGCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGtt                                                                                                                                                                                                              <  2:314126/251‑1 (MQ=255)                             aCGGCTCTGTGGGCGGGCGCTGCGGCGACGATCCTTTACACCTTTATTGGCGGTTTCCTCGCGGTGAGCTGGACTGGCACTGTACAGGCCAGCCTGATGATTTTTTCCCTGATCCTGACGCCGGGTATCGTCATTATCAGTGTCGGTGGCTTTTGGGGCTCGCTGGAAGAGATCAAACAAAAAAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTgggg                                                                                                                                                                                                          >  1:22893/1‑251 (MQ=255)                                      tgGGCGGGCGCTGCGGCGACGATCCTTTACACCTTTATTGGCGGTTTCCTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGTCCTGATCCTGACGCCGGGTATCGTCATTATCAGTGTCGGTGGGTTTGGTGACTCGCTGGGAGTGATCAAAAAAAAGAGCATCGAAAAAGTTTATATGCTCAAAGATCTGAACTTTTTTGCCATTATCTTACTGATGGGTTGGGGGCTGGGtt                                                                                                                                                                                                  >  1:81207/1‑250 (MQ=255)                                            ggcgCTGCGGCGACGATCCTTTGCCTCTGTATTGGCGGTTTCCTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTTACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCgg                                                                                                                                                                                           <  2:69866/251‑1 (MQ=255)                                                                                    tCCTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACGGATGGGTTGGGGGCTGGGTGACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATtc                                                                                                                                                    <  1:45311/250‑1 (MQ=255)                                                                                     ccTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATtctc                                                                                                                                                  >  1:364992/1‑251 (MQ=255)                                                                                        cgcgTTGATCTGGACTGACACTGTACAGGCCAGCCTGATCATTTTTGCCCTGATCCTGACGCCGGCTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGCTCCAACAAAAGAGCATCGAAAACGTTGATATGCTCAAATATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGTGCAGCCGCACTTTCTTGCGCGTTTTATGGTGGCCGATTCTcacc                                                                                                                                               >  2:229891/1‑251 (MQ=255)                                                                                             tGAGCTGGACTGACACTGGACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGTGTTTTATGGCGGGGGGTtctccacacagg                                                                                                                                          >  2:102399/1‑243 (MQ=255)                                                                                                         acacTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATgcg                                                                                                                              >  1:354166/1‑251 (MQ=255)                                                                                                          cacTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATgcgc                                                                                                                             >  1:433719/1‑251 (MQ=255)                                                                                                                   ggCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTa                                                                                                                    <  2:354166/251‑1 (MQ=255)                                                                                                                       aGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTAt                                                                                                                <  1:390527/251‑1 (MQ=255)                                                                                                                                            aTCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGGATCTCACCACAGCCTTGTCCCTGAGTGGCGTCTTAGTATGACCTGGATGCTCCTCTGCCt                                                                                           >  2:362684/1‑251 (MQ=255)                                                                                                                                                   cGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAggg                                                                                    <  2:171774/251‑1 (MQ=255)                                                                                                                                                   cGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAggg                                                                                    <  2:375636/251‑1 (MQ=255)                                                                                                                                                                         tgtCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTGTCGGCTTCtttt                                                              >  1:337912/1‑250 (MQ=255)                                                                                                                                                                          gtCGGTGGCTTGGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCTCAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTGTCGGCTTCTTTgg                                                             <  2:441193/251‑1 (MQ=255)                                                                                                                                                                            cGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGATCAAAGATCTGAACTTTGATGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGCATTAGTATGACCTGGATGATCCTCTGCCTGGCGGGGGCGGAGGCTGTCGGCTTCttttggg                                                           >  2:199601/1‑247 (MQ=255)                                                                                                                                                                             ggTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTTTGACCTGGATGATCCTCTGCCTGGCAGGGGCGGCGGGGGTTGGGTTCTTTgggtt                                                          >  2:251263/1‑249 (MQ=255)                                                                                                                                                                                ggCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTGTCGGCTTCTTTGGGATTGc                                                       <  1:89033/251‑1 (MQ=255)                                                                                                                                                                                         gACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTGTCGGCTTCTTTGGGATTGCTTACTTTa                                               <  1:16523/250‑1 (MQ=255)                                                                                                                                                                                             cGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTGTCGGCTTCTTTGGGATTGCTTACTTTAACGat                                          <  1:50637/251‑1 (MQ=255)                                                                                                                                                                                                  gAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTGTCGGCTTCTTTGGGATTGCTTACTTTAACGATCATcc                                     <  2:337912/251‑1 (MQ=255)                                                                                                                                                                                                                               cGTTGATATGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTTTCAACACAGCATTGTCCATGCGCGTCGAATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTGTCGGTTTTTTTGGGATTGTTTACTTTAAAGAGCATCCGGCGGTTGCTGGTGCGGGAAAttcgaa          >  1:274307/1‑244 (MQ=255)                                                                                                                                                                                                                                     tatGCTCAAAGATCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTTGCTGTCGGGTTTTTTGGGCGTGCCTACCTTTACGATCATCCGGCGGTGGCTGGTGCGGTGAATTAGAACGCCgggg  >  1:268197/1‑248 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                TGAAAGTACCTTTGGCATGAGCTACGAAACGGCTCTGTGGGCGGGCGCTGCGGCGACGATCCTTTACACCTTTATTGGCGGTTTCCTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCACTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTGTCGGCTTCTTTGGGATTGCTTACTTTAACGATCATCCGGCGTTGGCTGGTGCGGTAAATCAGAACGCCGAGC  >  NC_000913/1079760‑1080238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑