Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,099,700	G→A	D21N (GAT→AAT)	ycdY →	chaperone protein YcdY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,099,700	0	G	A	100.0%	164.8 / NA	48	D21N (GAT→AAT)	ycdY	chaperone protein YcdY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/18);  total (30/18)

ACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAGATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCC  >  NC_000913/1099461‑1099941                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  acCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTg                                                                                                                                                                                                                                        <  1:400219/251‑1 (MQ=255)  ccGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTgg                                                                                                                                                                                                                                       >  1:257420/1‑251 (MQ=255)       tttACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCg                                                                                                                                                                                                                                  >  1:140976/1‑251 (MQ=255)        ttACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGc                                                                                                                                                                                                                                 >  1:439463/1‑251 (MQ=255)                ggggAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTAcc                                                                                                                                                                                                                         >  2:149983/1‑251 (MQ=255)                                        aTCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCt                                                                                                                                                                                                 <  1:237138/251‑1 (MQ=255)                                                      aGATTTGCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACt                                                                                                                                                                                   <  1:200206/251‑1 (MQ=255)                                                          tttCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGa                                                                                                                                                                                <  1:40959/250‑1 (MQ=255)                                                                  agagCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGACATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATg                                                                                                                                                                        >  1:129698/1‑250 (MQ=255)                                                                         aTTTTGAATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTg                                                                                                                                                                >  2:132817/1‑251 (MQ=255)                                                                             tGAATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGacac                                                                                                                                                            >  2:173021/1‑251 (MQ=255)                                                                                      ctcCGCGCCGCGTACTGACCTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTGGCAGGCCTTTAATTACTAGTAATGGAGATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTGCACTCGATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCAGCTGAAGCAGGATGCGTTGCGGACACGTTTGc                                                                                                                                                      >  2:100178/1‑248 (MQ=255)                                                                                          gcgcCGCGTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTACTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAg                                                                                                                                               <  1:100178/251‑1 (MQ=255)                                                                                           cgccgcTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGc                                                                                                                                              <  1:64299/251‑1 (MQ=255)                                                                                             ccgcTGACTGAAATTCCTTGAAGCTCGCGGTATGGGACCGATGGCGGAATGTGCAGACCTGTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCGCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTGTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCtg                                                                                                                                            <  2:343313/251‑1 (MQ=255)                                                                                                     tGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGCGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATg                                                                                                                                     >  2:389893/1‑250 (MQ=255)                                                                                                      gAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACAGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGTATGAGTTGCTGATACGTTTGCAGAAAAGCTGTGATATGAc                                                                                                                                   >  2:34839/1‑251 (MQ=255)                                                                                                        aCTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGc                                                                                                                                 >  2:36171/1‑251 (MQ=255)                                                                                                         ttaccTTTAATATCGCGGTATTGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTATTGGAAATATATTAA‑TGAACGAGTTTTCTATCATCTATCGTGTGCTGGGGTCTCTCTATTACAGCCAACCACAAAATACTTGACGGATGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGTATTAGGTGCTGACACGCTTGCAGCAAAGCTGGGATATGAcac                                                                                                                                 >  2:226913/4‑248 (MQ=255)                                                                                                                    ctcGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGAGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAACCTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCACGTCTCTGCCg                                                                                                                     >  1:221899/1‑251 (MQ=255)                                                                                                                        cgGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑GGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGTGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTa                                                                                                                 <  2:295089/251‑1 (MQ=255)                                                                                                                         ggTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTAc                                                                                                                <  1:36171/251‑1 (MQ=255)                                                                                                                                        gCGGAATTTGCAGGCCTTTAATTTCTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCCCCGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTAt                                                                                                  <  2:90119/250‑1 (MQ=255)                                                                                                                                           gAATTTGCAGACCTTTAATTACTAGGAATGGAGATATATTAA‑TGAACGAGCTTTCTATCCTCTGTCGAGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGCTTCGTGAGGGGAAACTGGCTGCGAACTGGCAACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCCAGTCTCTGCCGATTACAATGCGTTGTTTATCGGc                                                                                              >  1:37832/1‑251 (MQ=255)                                                                                                                                              tttGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGAt                                                                                           >  1:260540/1‑251 (MQ=255)                                                                                                                                                  cAGACCTTTAATTAATAGTAATGGAAATATATTAAATGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGAGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATgc                                                                                         >  2:131262/1‑249 (MQ=255)                                                                                                                                                             ttACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTgccg                                                                            >  2:158273/1‑251 (MQ=255)                                                                                                                                                               aCTAGCAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGACGTGTGCTGGATTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGCAAAGCTGTGCTATGACGCAAGTCTCTGACGATTACAATGCGTTGTTTATCGACGATGAATGTGCTGTgccgcc                                                                          >  1:453194/1‑251 (MQ=255)                                                                                                                                                                cTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTgccgcc                                                                          >  1:43133/1‑250 (MQ=255)                                                                                                                                                                   gTAATGGAAATATATTAA‑TTAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCatat                                                                      <  2:140976/251‑1 (MQ=255)                                                                                                                                                                         gAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAgc                                                                >  2:352811/1‑251 (MQ=255)                                                                                                                                                                          aaaTATATTAC‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGACCACTGGCGCAGGATGAGTTGATGACAAGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATGCCAATGCGTTGTTTATCGACGATGAATGTGCTGTGCCGCCATATCgcaacg                                                               >  2:316382/1‑246 (MQ=255)                                                                                                                                                                                  tAA‑TGAACGAGTTTACTATCCTCTGCCGTGTGCTGGGTTCGCTCTATTACAGCAAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATAACGTAGCGCATGGGtt                                                       >  2:185794/1‑251 (MQ=255)                                                                                                                                                                                   aa‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGATCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTg                                                      >  1:25356/1‑251 (MQ=255)                                                                                                                                                                                            aGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCTTATCGTAGCGCATGGGTTGAGGGcgcg                                              >  1:35327/1‑251 (MQ=255)                                                                                                                                                                                            aGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGcgcg                                              >  2:133569/1‑251 (MQ=255)                                                                                                                                                                                                ttCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACTCGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGAcg                                           <  1:61139/250‑1 (MQ=255)                                                                                                                                                                                                    aTCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGAcggaagc                                      >  2:117460/1‑251 (MQ=255)                                                                                                                                                                                                    aTCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGAcggaa                                        <  1:149983/249‑1 (MQ=255)                                                                                                                                                                                                      ccTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTGCTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACGGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGAcggaagcg                                     <  1:185794/250‑1 (MQ=255)                                                                                                                                                                                                       cgcnGTCGGGTTCTGGGTGCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACTCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGAcggaagcgga                                   <  2:416983/249‑1 (MQ=255)                                                                                                                                                                                                            tCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTgc                              >  2:373531/1‑251 (MQ=255)                                                                                                                                                                                                                            ctctATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGttt                                                                                                    >  1:190754/1‑165 (MQ=255)                                                                                                                                                                                                                            ctctATTACCGCCAACCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGttt                                                                                                    <  2:190754/165‑1 (MQ=255)                                                                                                                                                                                                                              ctATTACCGCCAACCACAAAATCCTGTACTGGTGCCGCTGTTTACCCGGATTCGTGAGGGGAAACGGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAATCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCTTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGa              <  2:257420/249‑1 (MQ=255)                                                                                                                                                                                                                                        cAACCACAAAATCCTTTACTGTTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGTTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGcc  <  2:25356/251‑1 (MQ=255)                                                                                                                                                                                                                                        cAACCACAAAATCCTTTACTGGTGCCGCTGTTTACACTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGcc  >  1:172792/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaCCACAAAATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGcc  >  1:301442/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  ACCGCGTTTACCATGGGGGAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTGAATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCGGAATTTGCAGACCTTTAATTACTAGTAATGGAAATATATTAA‑TGAACGAGTTTTCTATCCTCTGTCGTGTGCTGGGTTCGCTCTATTACCGCCAACCACAAGATCCTTTACTGGTGCCGCTGTTTACCCTGATTCGTGAGGGGAAACTGGCTGCGAACTGGCCACTGGAGCAGGATGAGTTGCTGACACGTTTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCC  >  NC_000913/1099461‑1099941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑