Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,154,726	C→T	V237V (GTC→GTT)	mdtA →	multidrug efflux pump membrane fusion protein MdtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,154,726	0	C	T	100.0%	146.7 / NA	43	V237V (GTC→GTT)	mdtA	multidrug efflux pump membrane fusion protein MdtA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/22);  total (21/22)

AAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCG  >  NC_000913/2154481‑2154967                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  aaaaCCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCTGTGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTAc                                                                                                                                                                                                                                              <  1:257999/251‑1 (MQ=255)  aaaCCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTAcc                                                                                                                                                                                                                                             >  1:333939/1‑251 (MQ=255)     ccaagctagttgcccGCCAGGAGCTGGATGCTCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTGGACAGCGCGCAGCGGCAACGCGACTGGGGGCGGATTACCGCGCCAGTCGATGGTGGCGTTGGTCTCGAGCATGTTGATGTTTGTAACCAAATCTCCAGTGGTGATACCACCGGAATCGTGGTGATCACCCAGACTGAGTCTATCGATTTAGTTTTTGCCCTg                                                                                                                                                                                                                                          <  2:38309/239‑1 (MQ=255)     ccAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTg                                                                                                                                                                                                                                          >  1:246556/1‑251 (MQ=255)              tttCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGc                                                                                                                                                                                                                                 >  1:221766/1‑251 (MQ=255)                    gCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTATCCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATc                                                                                                                                                                                                                           <  1:44501/251‑1 (MQ=255)                    gCCAGGAGCTGGATGCCCAACAGGCGCGGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTGCCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGGGGTGATACCACCGGGATCGTGGTGATCGCCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATc                                                                                                                                                                                                                           <  2:181567/251‑1 (MQ=255)                       aGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCTCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTTGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCt                                                                                                                                                                                                                        <  2:267825/251‑1 (MQ=255)                                   cccAACAGGCGCTGGTCAGTGAATCCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGTGCAGCTGCACCTCGACTGGAGCCGTATTGCCGCACCAGGCGAGGGTCGCGTTGGTCTCAAGCAGGTTGATGGTGGTAACCAAATCTGCAGTGATGTTACCACCTTGATCGTGGTGATCACCCGGACGCATCCCATCGATTTAGTTTTTTCCCTGCCGTAAAGCCATAGCGCTTCCTTAGTGCat                                                                                                                                                                                                            <  2:211268/251‑2 (MQ=255)                                              cTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGc                                                                                                                                                                                                 >  1:94651/1‑251 (MQ=255)                                                        aaaCCGAAGACCCCATTCAGGATGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGCCGAGGGTCGCGATGGTCTCAAGCAGGTAGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGAACGTGGTGATAACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACAGTAGGGACGGCGAAGAGAACCGGAAAACCg                                                                                                                                                                                       >  2:378678/1‑251 (MQ=255)                                                            cGAAGGCACCATTCAGGCTGATGGAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCGCAAGCAGGCTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTAACGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCtgg                                                                                                                                                                                   >  2:330896/1‑251 (MQ=255)                                                                     cATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGa                                                                                                                                                                            >  2:115744/1‑249 (MQ=255)                                                                          aGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTgg                                                                                                                                                                     <  2:295195/251‑1 (MQ=255)                                                                            gCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCg                                                                                                                                                                                               >  1:221779/1‑223 (MQ=255)                                                                            gCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCg                                                                                                                                                                                               <  2:221779/223‑1 (MQ=255)                                                                                         gcGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCg                                                                                                                                                      <  2:3344/251‑1 (MQ=255)                                                                                           gTTGCCAGCGCGCAGCTGCACCTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGGTTTTACCCTGCCGGAAAGCGATATCGCTACCGTATTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAATCCTGGGATCGCACCAACTCgaa                                                                                                                                                    <  2:46306/251‑1 (MQ=255)                                                                                              gCCAACGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCgaaga                                                                                                                                                  <  1:409845/250‑1 (MQ=255)                                                                                                    gcgcAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTCCCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAg                                                                                                                                           <  2:246556/251‑1 (MQ=255)                                                                                                       cAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTg                                                                                                                                         >  2:252599/1‑250 (MQ=255)                                                                                                            gCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGc                                                                                                                                    >  1:315914/1‑250 (MQ=255)                                                                                                              aaCTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATCCCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACg                                                                                                                                 <  2:25075/251‑1 (MQ=255)                                                                                                              aaCTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACg                                                                                                                                 >  1:10206/1‑251 (MQ=255)                                                                                                                  cGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGt                                                                                                                             >  1:143282/1‑251 (MQ=255)                                                                                                                       ggAGCCTGTTTACCGCACCAGTAGATGGTCGCGTTGGTCTAAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATCCCCCCGGAATCGTGGTGAGCACCCAGACGCGTCCTAACGATTTAGTTTTTACCCTGCCGGAAAGCGATATCTCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAAACGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTGAAGt                                                                                                                        <  2:9889/251‑1 (MQ=255)                                                                                                                        gAGCAGGATTACCGGTCCAGGCGATTTTCGCGTTGGTATCAATCAGGTTGATGTTTGTTACCTAATCTCCATTTGTTATACCACCTGGATCGTTGTGATCTCTCAGACCCATCCTATCGATTTAGTTTTTCCCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCATAAAGCCGGAAAACCGCTTGTGGTAGATGCTTGGGATCGCACCAACTCGAATAAATTAAGTGAATGCACGCTGTTaattc                                                                                                                       <  2:371869/251‑4 (MQ=255)                                                                                                                           ccGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCt                                                                                                                      >  2:269153/1‑249 (MQ=255)                                                                                                                             ggATTACCGGCCCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGAt                                                                                                                  <  2:402987/251‑1 (MQ=255)                                                                                                                                       aCCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCg                                                                                                        >  1:256467/1‑251 (MQ=255)                                                                                                                                                         tGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACg                                                                                       >  1:401651/1‑250 (MQ=255)                                                                                                                                                               cAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAg                                                                                <  1:28466/251‑1 (MQ=255)                                                                                                                                                                 aGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTCCGATTACAGTg                                                                              >  1:16956/1‑251 (MQ=255)                                                                                                                                                                   cAGGTTGATGTTGGAAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGa                                                                             >  1:341480/1‑250 (MQ=255)                                                                                                                                                                    aGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCGGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGaaa                                                                           <  1:429148/251‑1 (MQ=255)                                                                                                                                                                                       aaTCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAgg                                                        >  1:114133/1‑251 (MQ=255)                                                                                                                                                                                            ccAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGga                                                       <  2:315914/247‑1 (MQ=255)                                                                                                                                                                                                    gATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGtt                                           <  2:333939/251‑1 (MQ=255)                                                                                                                                                                                                         caccGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCa                                      <  1:328142/251‑1 (MQ=255)                                                                                                                                                                                                                       tgATCACCCAGACGCATCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAAc                        >  1:109271/1‑251 (MQ=255)                                                                                                                                                                                                                                       tCCTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGATGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGAACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCg        >  1:22519/1‑251 (MQ=255)                                                                                                                                                                                                                                        ccTATCGATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGa       <  1:456648/251‑1 (MQ=255)                                                                                                                                                                                                                                              gATTTAGTTTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCg  >  1:5975/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  AAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCACTACCGGTACGATTAAAGTGAAAGCACGCTTTAATAATCAGGATGATGCGCTGTTTCCCAATCAGTTTGTTAACGCGCGCATGTTAGTCGACACCG  >  NC_000913/2154481‑2154967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑