Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,111,994	G→A	A378T (GCG→ACG)	opgH →	osmoregulated periplasmic glucans biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,111,994	0	G	A	100.0%	177.9 / NA	55	A378T (GCG→ACG)	opgH	osmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/24);  total (31/24)

CCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTG  >  NC_000913/1111755‑1112244                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                           ccTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATg                                                                                                                                                                                                                                                 >  1:405405/1‑251 (MQ=255)          aGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTATATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGAGCAGACCGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTg                                                                                                                                                                                                                                        >  1:68442/1‑251 (MQ=255)            cTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGt                                                                                                                                                                                                                                       >  1:353801/1‑250 (MQ=255)            cTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGt                                                                                                                                                                                                                                       >  1:344469/1‑250 (MQ=255)             ttATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGACAACGTCCGGTATGGATACGCTGTAt                                                                                                                                                                                                                                     >  2:62991/1‑251 (MQ=255)             ttATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTAt                                                                                                                                                                                                                                     >  2:257142/1‑251 (MQ=255)                tCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATgcg                                                                                                                                                                                                                                  >  1:74120/1‑251 (MQ=255)                  gCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATgcgcg                                                                                                                                                                                                                                <  1:59179/251‑1 (MQ=255)                              ggCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGtt                                                                                                                                                                                                                    >  1:130094/1‑251 (MQ=255)                                cGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTcg                                                                                                                                                                                                                  <  1:224385/251‑1 (MQ=255)                                      tCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGAccc                                                                                                                                                                                                            >  1:133574/1‑251 (MQ=255)                                          aTTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCgt                                                                                                                                                                                                        <  1:252872/251‑1 (MQ=255)                                                       gccgccgTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTg                                                                                                                                                                                           <  1:257142/251‑1 (MQ=255)                                                               cgccgcGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGc                                                                                                                                                                                   <  2:133574/251‑1 (MQ=255)                                                               cgccgcGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGc                                                                                                                                                                                   <  1:148223/251‑1 (MQ=255)                                                                   gcgTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGt                                                                                                                                                                               >  1:391031/1‑251 (MQ=255)                                                                      tGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGGGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGGATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTg                                                                                                                                                                            >  2:342809/1‑251 (MQ=255)                                                                       gAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTg                                                                                                                                                                            <  2:374713/250‑1 (MQ=255)                                                                        agtcGTAAAAGCGGTAATATCGTTGCCTTCGGCCGTCGCTGGGGCAGGCAGTACATCTACATGGTGGTGCTGGATGCTGTCTGGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTCCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGGTTACAGCTGGTTTgaa                                                                                                                                                                          <  2:267400/248‑3 (MQ=255)                                                                                 aGCGGTAATATCGATGACTTCGGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCa                                                                                                                                                                 <  1:127066/251‑1 (MQ=255)                                                                                     gTAATCTCGATGCCTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTAGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGATTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGACCGCCGGGATCCTTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGAGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACtt                                                                                                                                                             >  2:436102/1‑251 (MQ=255)                                                                                       aaTATCGATGACTTCGGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGTTACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTg                                                                                                                                                            <  2:375844/250‑1 (MQ=255)                                                                                        atatCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGCCCCGAGTGTATGAGCCAATGTTTACAGACGGTTTGCACTTCTGGCAACTTGGc                                                                                                                                                          >  2:335027/1‑251 (MQ=255)                                                                                            cGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGTTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAg                                                                                                                                                       <  1:322123/250‑1 (MQ=255)                                                                                             gATGACTTCTGCCGTCGCTGGGGCAGCCAGTACATCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGTTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGACCGGTAGGGATACGCTGTATTCGCGCTGTCAGCAGTTCGCAACCCGCGTGTATGAGCCACTGTTTACAGCCGGTTTGCGCTTCTGGCAACTGGGCGAGTc                                                                                                                                                     <  2:445243/251‑1 (MQ=255)                                                                                                       gCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGAGCTGTCAGCAGTTCGCGACCCGCGTGTCTGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCCATACTgg                                                                                                                                           >  1:214977/1‑251 (MQ=255)                                                                                                           gcgcgggggCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGAc                                                                                                                                       <  2:99353/246‑1 (MQ=255)                                                                                                                ggtgCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGGCCGGTATGGATACGCTGTATGCGCGCTGTCCGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCTAGTCGCACTACTGGGGACATAAc                                                                                                                                  >  2:129025/4‑251 (MQ=255)                                                                                                                gggnCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATGCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAAc                                                                                                                                  <  2:405405/251‑1 (MQ=255)                                                                                                                ggggCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAAc                                                                                                                                  >  1:113722/1‑251 (MQ=255)                                                                                                                            aCAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCcg                                                                                                                       <  2:202393/250‑1 (MQ=255)                                                                                                                             cAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCcgcg                                                                                                                     >  2:380622/1‑251 (MQ=255)                                                                                                                                    aTGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAAcc                                                                                                              >  1:89035/1‑251 (MQ=255)                                                                                                                                    aTGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAAcc                                                                                                              <  1:245907/251‑1 (MQ=255)                                                                                                                                      ggtggtGCTGGATGCTGACTTGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGt                                                                                                            <  1:62991/251‑1 (MQ=255)                                                                                                                                        tggtgCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGc                                                                                                                                                          >  2:359364/1‑203 (MQ=255)                                                                                                                                        tggtgCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGc                                                                                                                                                          <  1:359364/203‑1 (MQ=255)                                                                                                                                                              aaTGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCTTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTc                                                                                    <  1:363027/251‑1 (MQ=255)                                                                                                                                                                  aCCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAAAGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCt                                                                                >  2:307712/1‑251 (MQ=255)                                                                                                                                                                     ggTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCCCTGCGCACTGGCTCCGCTGcc                                                                             >  2:357924/1‑251 (MQ=255)                                                                                                                                                                                   cGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGCGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCt                                                               >  2:267917/1‑251 (MQ=255)                                                                                                                                                                                        tGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGTTCATTCAGTCGTCGCCGCAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGAGTATGGGACAATGTTTACAGCAGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGAGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCAGCTGCCGGGCGAAGGTTCCTTTGcc                                                          >  1:258365/1‑251 (MQ=255)                                                                                                                                                                                               ccTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCa                                                    >  1:367127/1‑250 (MQ=255)                                                                                                                                                                                                   aTGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCt                                               >  1:49603/1‑251 (MQ=255)                                                                                                                                                                                                         gCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCt                                                               >  1:316391/1‑229 (MQ=255)                                                                                                                                                                                                         gCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCt                                                               <  2:316391/229‑1 (MQ=255)                                                                                                                                                                                                              cccNAACGCCGGGATAATGCGGTCGTCGCCGAAAACGTCCGGTAGGGATCCGCTGTATGCGCGGTGTCAGCAGGTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTGGGCGAGTCGCACTACTGGTGACATAACGCGATTATCCTCGTGAAACCGTTTATCGAGCACTGCGCACGGGTTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTGCAATCCTGTCACATGACt                                    <  2:411115/251‑1 (MQ=255)                                                                                                                                                                                                                  aaCGCCGGGATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGt                                >  1:449564/1‑251 (MQ=255)                                                                                                                                                                                                                          gATCATTCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAgcgg                        <  2:52836/251‑1 (MQ=255)                                                                                                                                                                                                                               ttCAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGCCTTCGTGGAAGCGGCGTTg                   >  2:137505/1‑251 (MQ=255)                                                                                                                                                                                                                                 cAGTCGTCGCCGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCTTGGAAGCGGCGTTGa                  <  1:340014/250‑1 (MQ=255)                                                                                                                                                                                                                                     cgtcgCCGCAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGATGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATgcgc             >  2:42498/1‑251 (MQ=255)                                                                                                                                                                                                                                          ccGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGc        >  1:199301/1‑251 (MQ=255)                                                                                                                                                                                                                                           cGAAAACGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGc        >  2:449173/1‑250 (MQ=255)                                                                                                                                                                                                                                           cGAAAACGTCCGGGATGGATACGCTGTAGGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGTGCCACTGTTTCCAGCCGGTTTGTACTTCTGGCAACTTGGCGAGTCGTACTATTGGGGACATAACGCGTTTATCCGTGTGAAACCGTTTATCGAGTACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCa       <  1:26080/251‑1 (MQ=255)                                                                                                                                                                                                                                                gCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTg  <  2:94834/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                           CCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTG  >  NC_000913/1111755‑1112244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑