Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	464,699	G→A	A100T (GCG→ACG)	fadM →	long‑chain acyl‑CoA thioesterase FadM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	464,699	0	G	A	100.0%	63.8 / NA	20	A100T (GCG→ACG)	fadM	long‑chain acyl‑CoA thioesterase FadM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/10);  total (10/10)

CAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATGGGTTGGAAAATAGCGACAGTTTTCAGTGGATGACGGCCCATAACATCGCCTTCGTCGTGGTCAATATCAATATTAACTATCGTCGCCCAGCGGTATTAAGTGACCTGTTAACTATTACCAGTCAGTTGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAGCGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAA  >  NC_000913/464455‑464921                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                               cancaaCGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATGGGTTGGAAAATAGCGACAGTTTTCAGTGGATGACGGCCCATAACATCGCCTTCGTCGTGGTCAATATCAATATTAACTATCGTCGCCCAGCGGTATTAAGTGACCTGTTAACTATTACCAGTCAGTTGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGTAGCCGGAAGGGCAGGTGGTAACGGAt                                                                                                                                                                                                                           <  2:408798/250‑1 (MQ=255)                             gaagaaGCCCGCTGGGATGGGTTGGAAAATAGCGACAGTTTTCAGTGGATGACGGCCCATAACATCGCCTTCGTCGTGGTCAATATCAATATTAACTATCGTCGCCCAGCGGTATTAAGTGACCTGTTAACTATTACCAGTCAGTTGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCt                                                                                                                                                                                              <  2:30365/251‑1 (MQ=255)                                            gATGGGTTGGAAAATAGCGACAGTTTTCAGTGGATGACGGCCCATAACATCGCCTTCGTCGTGGTCAATATCAATATTAACTATCGTCGCCCAGCGGTATTAAGTGACCGGTTAACTATTACCAGTCAGTTGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGc                                                                                                                                                                               <  1:421148/251‑1 (MQ=255)                                             aTGGGTTGGAAAATAGCGACAGTTTTCAGTGGATGACGGCCCATAACATCGCCTTCGTCGTGGTCAATATCAATATTAACTATCGTCGCCCATCGGTATTAAGTGACCTGTTAACTATTACCAGTCAGTTGCAGCAATTAAGCGGTAAAAGCGGCATCTTAAGCCAGGTCATTACGCTGGAGCCGGAAGGGCAGGTGGTAACGTATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCa                                                                                                                                                                              <  1:279350/251‑1 (MQ=255)                                                                                aaggctcATAACATCGCCTTCGACGTGGTCAATATCAATATTAACGATCGTATCCCAGCGGTATTAAGTGACCTGTTAACTATTACCAGTCAGATGCAGCAATTAAACGGAAACAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCAGGAAGGGCAGGTGATAACGGCCGCGCTTATTACGTATGTTTGTATTGATATTAAAACGAAGCACGCATTAGCTCTGGAAGGTGAATTACGCGAAAAgat                                                                                                                                              >  2:232555/7‑246 (MQ=255)                                                                                aCGGCCCATAACATCGACTTCGTCGTGGTCAATATCAATATTAACTATCGTCGCCCAGCGGTATTAAGTGACCTGTTAACTATTACCAGTCAGTTGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGa                                                                                                                                           <  2:75970/251‑1 (MQ=255)                                                                                                    cgtcgtGGTCAATATCAATATTAACTATCGTCGCCCAGCGGTATTAAGTGACCTGTTAACTATTACCAGTCAGTTGCATCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGt                                                                                                                       <  1:390337/251‑1 (MQ=255)                                                                                                             caatatcaatatTAACTATCGTCGCCCAGCGGTATTAAGTGACCTGTTAACTATTACCAGTCAGTTGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTgtggtg                                                                                                              >  2:171404/1‑251 (MQ=255)                                                                                                                 atcaatatTAACTATCGTCGCCCAGCGGTATTAAGTGACCTGTTAACTATTACCAGTCAGTTGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCgg                                                                                                          <  1:169858/251‑1 (MQ=255)                                                                                                                 atcaatatTAACTATCGTCGCCCAGCGGTATTAAGTGACCTGTTAACTATTACCAGTCAGTTGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCgg                                                                                                          >  2:110605/1‑251 (MQ=255)                                                                                                                      tatTAACTATCGTCGCCCAGCGGAATTAAGAGACCTGTTAACTATTACCAGTCAGTTGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCt                                                                                                     >  1:103302/1‑251 (MQ=255)                                                                                                                          aaCTATCGTCGCCCAGCGGTATTAAGTGACCTGTTAACTATTACCAGTCAGTTGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCa                                                                                                   >  1:61837/1‑249 (MQ=255)                                                                                                                                            gTATTAAGTGACCTGTTAACTATTACCAGTCAGTTGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACAccc                                                                               >  2:449516/1‑251 (MQ=255)                                                                                                                                                                        gtcagtTGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGTATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCta                                                   <  2:111841/251‑2 (MQ=255)                                                                                                                                                                              tGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTACTTCAt                                             >  1:303123/1‑251 (MQ=255)                                                                                                                                                                                         aaCGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGACGGGTGGTAACGGATGCGCTTATTACATTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGa                                                                                                                                                             >  2:297516/1‑128 (MQ=255)                                                                                                                                                                                           cGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTACTTCATCGCTTCCATCAcc                                <  1:87897/251‑1 (MQ=255)                                                                                                                                                                                                                  aGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTACTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATg         >  1:42696/1‑251 (MQ=255)                                                                                                                                                                                                                      cATTACACTGGAGCCGGAAGGGCAGGTGGTAACGGATGCTCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGCAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTACTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTc     >  2:137788/1‑251 (MQ=255)                                                                                                                                                                                                                          acacTGGAGCCGGAAGGGCAGGTGGTAACGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGTTTTTCTACTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCaaa  <  2:148385/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                               CAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATGGGTTGGAAAATAGCGACAGTTTTCAGTGGATGACGGCCCATAACATCGCCTTCGTCGTGGTCAATATCAATATTAACTATCGTCGCCCAGCGGTATTAAGTGACCTGTTAACTATTACCAGTCAGTTGCAGCAATTAAACGGTAAAAGCGGCATCTTAAGCCAGGTCATTACACTGGAGCCGGAAGGGCAGGTGGTAGCGGATGCGCTTATTACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCTGGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAA  >  NC_000913/464455‑464921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑