Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,353,758	T→A	D254V (GAT→GTT)	sapC ←	putrescine ABC exporter membrane protein SapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,353,758	0	T	A	100.0%	144.3 / NA	45	D254V (GAT→GTT)	sapC	putrescine ABC exporter membrane protein SapC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (28/17);  total (28/17)

AACAAGACCGCGGATTTCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACC  >  NC_000913/1353512‑1354000                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    aacaaGACCGCGGATTTCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACAcc                                                                                                                                                                                                                                                 <  2:204683/251‑1 (MQ=255)                             aaagacATACTTACCCGGTCGACGGCTTTAACCCAATCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGTGTAATGGCATTATTCCACCCACGAAATAATCGCACGACGAACGCCATCGCCAAGGAAGTTAACAAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACCTAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAnggg                                                                                                                                                                                                                    >  2:412777/6‑251 (MQ=255)                             aaGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAggg                                                                                                                                                                                                                     <  2:321635/250‑1 (MQ=255)                               ggTCATACTTCCCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTACCCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAgggg                                                                                                                                                                                                                    >  2:33699/1‑249 (MQ=255)                                              gTCGACGGCTTTAACCCACTCATCACCGGCTTTAAATTCAATGGTCAGGTAACGAATATCGAGTAATGGCATTATTACACCCCCGCACTAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGCGTTGTGCGCCg                                                                                                                                                                                                        >  2:43736/1‑246 (MQ=255)                                                 gACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCgag                                                                                                                                                                                                <  2:29652/251‑1 (MQ=255)                                                      cTTTTACCCACTCATCACCGTTTTTAAATTCAATGTTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACTCGCTAATCATAATTGCCGCACCTGACAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCgag                                                                                                                                                                                                      <  1:63884/240‑1 (MQ=255)                                                      cTTTAACAAACTCACCACCGGTTTTAAATTCAATGGTCAGGCTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACATGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATGC‑AGGCGCGGGGAAGTGTGCGCCgag                                                                                                                                                                                                      >  2:63884/1‑240 (MQ=255)                                                                          gTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCg                                                                                                                                                                          >  1:259777/1‑248 (MQ=255)                                                                            tttAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATACTCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAAt                                                                                                                                                                     >  2:118537/1‑251 (MQ=255)                                                                                        ggTCAGTTTATGAATATCGAGTAATGGCATTCTTCCGCCCCCGCAGTAATCGCACGACGAACGCCATAGCCAAGGAGGTTCACCAACAACACCCTAATCATAATTACCGCACCTCGCAGCATGACATTCCACGGGGCGACAAAAATCCGTTCCAACGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGCGCAGTTGTGAGCCGAGATCGAGATAGCCCAGCGCGGCGATATCGAGAATTGCCATctcc                                                                                                                                                           >  2:269184/1‑246 (MQ=255)                                                                                        ggTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAGCCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACa                                                                                                                                                          <  2:96793/250‑1 (MQ=255)                                                                                        ggTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGCATTGCCATCGAc                                                                                                                                                           >  2:212066/1‑249 (MQ=255)                                                                                        ggTCAGGTTACGAAAATAGAGTAATGGCATTATTCCCCCCCAGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAAACCGCTAATAATACTTGCCGCACCTGGCAGCATGACAGTCCACGGAGCGACATAAATCAGATCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGCGGCGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATAGAGCATTGACATCGACa                                                                                                                                                          >  2:199246/1‑250 (MQ=255)                                                                                                   gAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCCTCGACAGTGCGCGGGTg                                                                                                                                               >  2:296018/1‑250 (MQ=255)                                                                                                   gAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCAAGGGGAGTTGTGCGCCGAGATAGAGAAAGCCCAGCCCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTg                                                                                                                                               >  2:283988/1‑250 (MQ=255)                                                                                                                    attattCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCcg                                                                                                                             >  1:123117/1‑251 (MQ=255)                                                                                                                      tattCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCcggc                                                                                                                           >  2:3189/1‑251 (MQ=255)                                                                                                                       attCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCcggc                                                                                                                           <  1:119635/250‑1 (MQ=255)                                                                                                                          ccACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCTCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTg                                                                                                                       >  1:271205/1‑251 (MQ=255)                                                                                                                              ccccGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑a                                                                                                                                                                                                                             >  2:398110/1‑145 (MQ=255)                                                                                                                              ccccGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑a                                                                                                                                                                                                                             <  1:398110/145‑1 (MQ=255)                                                                                                                              ccccGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGt                                                                                                                   <  1:3189/251‑1 (MQ=255)                                                                                                                                         tCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATcac                                                                                                         >  1:48362/1‑250 (MQ=255)                                                                                                                                                acgaACGCAATCGCAAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACCCCGAGCATCGCTCCCCCTTCGAGAC‑AGGGGAGTTGTGCGCCGAGATCGACAAAGCCCAGCGCGGCGAGCTCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGCTGACCCACCCGGCGGTGCTGCTTGGCAtgtcaggaaaac                                                                                                 >  2:191383/1‑240 (MQ=255)                                                                                                                                                            gCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGACGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGAATCACAGCAAACCAGAGAATATTc                                                                                      >  1:458793/1‑250 (MQ=255)                                                                                                                                                                             acaacaCGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGGCATcc                                                                    <  1:360686/251‑1 (MQ=255)                                                                                                                                                                                 cacGCTAATCATAATTGCCGCACCTGGCAGCATGACACTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGACGTTTGGCATCTCAGCAAACCAGAGAATATTCAGAGTTGATGCGCCATCAAGAc                                                                >  2:288504/1‑251 (MQ=255)                                                                                                                                                                                    gCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACggg                                                             <  1:263337/251‑1 (MQ=255)                                                                                                                                                                                       aaTCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGGGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGggcg                                                           <  1:64695/250‑1 (MQ=255)                                                                                                                                                                                        aTCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGggcggc                                                         >  2:61833/1‑251 (MQ=255)                                                                                                                                                                                         acATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGgcggcg                                                        <  2:394117/250‑1 (MQ=255)                                                                                                                                                                                             aaTTGCCGCACCTGGCAGCATGACCGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑CGGCGAGGGGAGTCGTCCGCCGAGATCGAGAAAGCCCAGCGAGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCAGCGGTGATGTTTCGGATCAAAGCAAACCATGGAAAATTCAGAGTTGACGCGCCATCCAGACGggcggacata                                                     >  2:195550/1‑245 (MQ=255)                                                                                                                                                                                                tGCCGCACCTGGCAGCATGACAGTCCTCGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTTTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGATAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACg                                                  <  1:185260/250‑1 (MQ=255)                                                                                                                                                                                                 gCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACgg                                                 >  1:204498/1‑249 (MQ=255)                                                                                                                                                                                                 gCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAACCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAACGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGCGATCTCGCTGACCAGCCCGGCGGTGATGTTTGGCCCCACAGCAAACCAGAGAACAATCATCGATGATGAGCCATCGAGACGGGCGGCGACAAccg                                                 >  2:54078/1‑248 (MQ=255)                                                                                                                                                                                                      aCCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAAAGTACTCtt                                           >  2:338439/1‑251 (MQ=255)                                                                                                                                                                                                                        ccACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGGTGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGcc                         >  2:157616/1‑250 (MQ=255)                                                                                                                                                                                                                                cGACATAGATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGGTGTGCGCCGGGAGCGAGAAAGCCCAGGGCGGCGATAGCGAGAATGGCCATCGACAGTg                                                                                                                                                       <  2:65319/117‑1 (MQ=255)                                                                                                                                                                                                                                cGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTg                 <  1:157616/251‑1 (MQ=255)                                                                                                                                                                                                                                  aCATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGCTAACTTAATCTTTTTCCAGTTCGTCATGCCCCCTGCTGt                >  1:395799/1‑250 (MQ=255)                                                                                                                                                                                                                                          cAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAAc       <  1:43736/251‑1 (MQ=255)                                                                                                                                                                                                                                           aGTTCCAGCGCAACACCGAGCATCGGTCCCCATTC‑ACGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTGGGCATCACAGGAAACCAGAACATATTCAGAGTTGATTCGGCATCCAGTCGGGCGGGGATAACGTACTCTTTTTCTAGTTCGTCATGCACCCTGCTGTAAATCCAACg      >  2:244100/1‑251 (MQ=255)                                                                                                                                                                                                                                            gTTCCAGCGCAACACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGAGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGGTAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGt     >  1:312724/1‑251 (MQ=255)                                                                                                                                                                                                                                               ccAGCGCAACACCGATCATCGCTCCCCATTC‑AGGCGAGGGGAGTGGTGCGACGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGGTCGTCATGCACCATGCTGTAAATCGAACGTAcc  <  1:369032/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    AACAAGACCGCGGATTTCACCTTCGGTTAAGGTCATACTTACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTC‑AGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACC  >  NC_000913/1353512‑1354000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑