Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	996,646	T→C	Y100C (TAC→TGC)	ssuA ←	aliphatic sulfonate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	996,646	0	T	C	100.0%	151.8 / NA	44	Y100C (TAC→TGC)	ssuA	aliphatic sulfonate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/22);  total (22/22)

AACGTTACCTTGCTGGAACGCGGCGCGGGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAGGATTCTGCAGCAACCG  >  NC_000913/996414‑996889                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    aaCGTTACCTTGCTGGAACGCGGCGCGGGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCgg                                                                                                                                                                                                                                   <  1:29592/251‑1 (MQ=255)   cGTTACCTTGCTGGAACGCGGCGCGGGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGc                                                                                                                                                                                                                                  >  1:293525/1‑250 (MQ=255)                      ggcgcggGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGGACGCAGTAAAAGGTTGTGGGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCGGCCACCAGAATCACTTCGGCTTTGGGCTTCTGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCg                                                                                                                                                                                                              <  1:452117/251‑1 (MQ=255)                         gcggGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCATCTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTCCGGCTTTGGGCTTCGGTTGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGaa                                                                                                                                                                                                           <  1:416421/251‑1 (MQ=255)                                        cGTCAGGTAAGTGGGTTAGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAACGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGACCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGc                                                                                                                                                                                            >  2:133833/1‑251 (MQ=255)                                           cAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACGTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCga                                                                                                                                                                                         <  2:360887/251‑1 (MQ=255)                                              gTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCg                                                                                                                                                                                          >  1:314260/1‑247 (MQ=255)                                                   tGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAAt                                                                                                                                                                                  >  1:358525/1‑250 (MQ=255)                                                      gTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTg                                                                                                                                                                              <  1:449046/251‑1 (MQ=255)                                                           atatCGGTAAACGTAAGTACGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTGTAAGATCGGCTACGGTTTTGATCGGGCTGTTTGCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATGGGCGGAATATCCCAGGTACTGCCGAGAGCAATACTGCCAAc                                                                                                                                                                         <  2:53655/251‑1 (MQ=255)                                                                ggTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTa                                                                                                                                                                    <  1:394599/251‑1 (MQ=255)                                                                  tAAACTTAAGTCCGGCCTGACGCAATGCACGCAATAAAAGGTTGAGTGAACTGGAACCTTTCTGAAATGCAACTTTGTGACCTTTAAGTTCGGCTACGGTTTTGTTAGGGATGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGATTCGGTGGCTAGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGAAAAGAATGGCGGAATATCCCCGGTACTGCCGAGATGCACAATGCCAACGTTTAAc                                                                                                                                                                  >  2:426965/1‑251 (MQ=255)                                                                         aaGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTcc                                                                                                                                                            >  1:129801/1‑250 (MQ=255)                                                                          aGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCa                                                                                                                                                           >  2:451135/1‑250 (MQ=255)                                                                               ggCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACAttt                                                                                                                                                     >  1:259286/1‑251 (MQ=255)                                                                                gCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATTCTGCCAACGTTTAACGCTTCCATCATTTg                                                                                                                                                    <  1:127939/251‑1 (MQ=255)                                                                                    gtcGCAGTGCACGCAGTAAAAGGCTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGACAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCgt                                                                                                                                                 >  2:297380/3‑249 (MQ=255)                                                                                                 cAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACt                                                                                                                                   >  2:379968/1‑251 (MQ=255)                                                                                                        aGGTTGTGTGAACTGGAGGCTTTCTGAAAGGCATCTTTGTGACCTTTAAGATCGGCGACGGTTTTGATCGGGCGGTTTTCTGCCTCCAGAATCACGTCGGCTTTGGGCTTTGGTGGCTCGACGCCCACGCGCGCCAGAGCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATAGCCCCGGTACTGCCGAGATGAAGGCTCCGAAGCTTTAACGCTTCCAACATTGGCGGACCCGCGGGGAACTCCACCCa                                                                                                                            <  1:425827/251‑1 (MQ=255)                                                                                                        aGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCa                                                                                                                            <  1:453619/251‑1 (MQ=255)                                                                                                                                     ggCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGttttt                                                                                               >  1:179196/1‑251 (MQ=255)                                                                                                                                         aCTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAg                                                                                           <  1:198878/251‑1 (MQ=255)                                                                                                                                              gtgACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACt                                                                                      <  1:297380/251‑1 (MQ=255)                                                                                                                                               tgACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTTCCAACGTTTa                                                                                                                                                                    <  2:412802/172‑1 (MQ=255)                                                                                                                                               tgACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTa                                                                                                                                                                    >  1:412802/1‑172 (MQ=255)                                                                                                                                               tgACCGTTAAGATCGGTTACGGTTTTGAGCGGGCAGTTTGCGGCCACCAAAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACAAAACCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAAAAGCGCCGGGACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGAATCCGGATAGCGTTTTTCCAGTAACt                                                                                      <  2:329859/250‑1 (MQ=255)                                                                                                                                                        agagcGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGAt                                                                                                        <  1:193979/219‑1 (MQ=255)                                                                                                                                                        aGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGCACTCCCCCCAGGAGCTTTTTGATTCCGGAt                                                                                                        >  2:193979/1‑223 (MQ=255)                                                                                                                                                               cTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGc                                                                     >  1:385797/1‑251 (MQ=255)                                                                                                                                                                  cGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCAcc                                                                  <  1:95254/251‑1 (MQ=255)                                                                                                                                                                   ggTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACg                                                                                                                                                                        >  2:333698/1‑148 (MQ=255)                                                                                                                                                                   ggTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACg                                                                                                                                                                        <  1:333698/148‑1 (MQ=255)                                                                                                                                                                            cGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATa                                                         <  2:349094/250‑1 (MQ=255)                                                                                                                                                                                 ggTNTTCTGCCACCAGAATCACTTCGGCTTTGGTCTTCGGTGGCTCTACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTTCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGAGAGCGTTTTTCCAGTAACTGGTGGCTTGTTGCCAGCACCATACCAATACTGCCt                                                   <  2:418241/250‑1 (MQ=255)                                                                                                                                                                                      tCTGCCACCAGAATGACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCAGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTg                                              >  2:206098/1‑251 (MQ=255)                                                                                                                                                                                           caccaGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACtg                                                                                     >  1:385484/1‑207 (MQ=255)                                                                                                                                                                                           caccaGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACtg                                                                                     <  2:385484/207‑1 (MQ=255)                                                                                                                                                                                           caccaGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGc                                         <  1:451135/251‑1 (MQ=255)                                                                                                                                                                                               aGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCtat                                     >  2:308525/1‑251 (MQ=255)                                                                                                                                                                                                                   cTTCGGTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAgg                 >  2:313605/1‑251 (MQ=255)                                                                                                                                                                                                                   cTTCGGTGGCTCGACGCCCACGCACACCAAATCAGACCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAAGTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTAAACGTAACGCTTCAGGCGAgg                 >  2:313613/1‑251 (MQ=255)                                                                                                                                                                                                                        gTGGCTCGACGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAGGATTCt            <  2:179196/251‑1 (MQ=255)                                                                                                                                                                                                                                 cGCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAGGATTCTGCAGCAAcc   >  1:169183/1‑251 (MQ=255)                                                                                                                                                                                                                                  gCCCACGCACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAGGATTCTGCAGCAACCg  >  2:362412/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    AACGTTACCTTGCTGGAACGCGGCGCGGGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTCTGCCACCAGAATCACTTCGGCTTTGGGCTTCGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAGGATTCTGCAGCAACCG  >  NC_000913/996414‑996889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑