Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	930,390	G→A	N191N (AAC→AAT)	cydD ←	glutathione/L‑cysteine ABC exporter subunit CydD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	930,390	0	G	A	98.0%	155.0 / NA	49	N191N (AAC→AAT)	cydD	glutathione/L‑cysteine ABC exporter subunit CydD
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (30/18);  total (30/19)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

ATAGGAAAAACCAAAGTAGACCGCCACCAGAGCAATTGACAGCGAGGTAAAAAATTCGAGAATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTG  >  NC_000913/930147‑930620                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       aTAGGAAAAACCAAAGTAGACCGCCACCAGAGCAATTGACAGCGAGGTAAAAAATTCGAGAATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCAt                                                                                                                                                                                                                                 <  2:32523/251‑1 (MQ=255)  tAGGAAAAACCAAAGTAGACCGCCACCAGAGCAATTGACAGCGAGGTAAAAAATTCGAGAATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATc                                                                                                                                                                                                                                >  1:194351/1‑251 (MQ=255)              aaGTAGACCGCCACCAGAGCAATTGACAGCGAGGTAAAAAATTCGAGAATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCAt                                                                                                                                                                                                                    <  2:166562/251‑1 (MQ=255)                       gCCACCAGAGCAATTGACAGCGAGGTAAAAAATTCGAGAATGCCGGAGGATAAAAAAGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACAAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGAGAGCAAGAGCGAGAAAGTTACGTAGATTAGAATCGGCAGCCCCCATTCCAAcc                                                                                                                                                                                                             >  1:224156/1‑249 (MQ=255)                          accaGAGCAATTGACAGCGAGGTAAAAAATTCGAGAATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAgcgc                                                                                                                                                                                                        >  2:131463/1‑251 (MQ=255)                                        cAGCGAGGTAAAAAATTCGAGAATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGa                                                                                                                                                                                           >  1:93673/1‑250 (MQ=255)                                              ggTAAAAAATTCGAGAATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACg                                                                                                                                                                                    >  1:381808/1‑251 (MQ=255)                                                    aaaTTCGAGAATGCCGGAGGCTAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGACCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGc                                                                                                                                                                                >  2:250545/1‑249 (MQ=255)                                                     aaTTCGAGAATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGt                                                                                                                                                                             >  1:242277/1‑251 (MQ=255)                                                         cGAGAATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAACCAACGGAATTAACGGTGCAGTg                                                                                                                                                                            >  2:455253/1‑248 (MQ=255)                                                         cGAGAATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTg                                                                                                                                                                            <  1:455253/248‑1 (MQ=255)                                                         cGAGAATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGccc                                                                                                                                                                         >  1:364515/1‑251 (MQ=255)                                                           agaATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGATGGAGGAAATCTTCCGAAGCCGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATACCGCGCAGGCGATCGAGGAAATGCCCACTTAACCGAGCCAGAGCGAGAAAGTTACGTCGATTAACATCGGCAGCCCCCATTCCAACAACAGCCATAAACAACGAAATTAACGATGCAGTGccc                                                                                                                                                                         >  2:319723/1‑249 (MQ=255)                                                            gaATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCcagc                                                                                                                                                                      <  1:455541/251‑1 (MQ=255)                                                                    gaggaTAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATg                                                                                                                                                                >  1:345325/1‑249 (MQ=255)                                                                     aggaTAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAgc                                                                                                                                                             <  1:241183/251‑1 (MQ=255)                                                                      ggaTAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAAGGAgcg                                                                                                                                                            <  1:367091/251‑1 (MQ=255)                                                                                 ccAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGACTACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCACAGCAGAATGAGCGCCGCAGccc                                                                                                                                                   >  1:306264/1‑249 (MQ=255)                                                                                  cAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGt                                                                                                                                                <  1:310349/251‑1 (MQ=255)                                                                                                  aTTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGc                                                                                                                                >  2:378206/1‑251 (MQ=255)                                                                                                      tCCGTTGGCGGAAATCTTCCGAAGCCGAACGAATAATTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCAAGCAGAATGAGCGACGCAGCCCAGTTAGAGGGGAAGATTGccacc                                                                                                                            >  1:115841/1‑251 (MQ=255)                                                                                                                 aaaTCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCa                                                                                                                  >  2:378485/1‑250 (MQ=255)                                                                                                                          gAAGCAGAACGAATACTTTCAATTTCAGCTTCACCCCGACCAAAAATACGCCATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCg                                                                                                         >  2:266974/1‑250 (MQ=255)                                                                                                                              cAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACt                                                                                                    >  2:33560/1‑251 (MQ=255)                                                                                                                                 aaCGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGcc                                                                                                 >  2:393241/1‑251 (MQ=255)                                                                                                                                             cAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGc                                                                                     >  2:268770/1‑251 (MQ=255)                                                                                                                                              aaTTTCAGCTTCACCACGACCAAATATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTATAGGGGATGATTGCTACCACAATCAGCAACGGCACCGACACTGCCCGCGCCATTTGct                                                                                    >  2:13843/1‑250 (MQ=255)                                                                                                                                              aaTTTCACCTTCACCACGACCAAAAATACTCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCg                                                                                    <  2:52927/251‑1 (MQ=255)                                                                                                                                                        tCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGACTTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCg                                                                          >  1:265281/1‑251 (MQ=255)                                                                                                                                                          accacGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCGCTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAAGCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTg                                                                        <  2:22060/251‑1 (MQ=255)                                                                                                                                                              cGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCAt                                                                     >  1:247572/1‑250 (MQ=255)                                                                                                                                                               gACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCAt                                                                     >  1:450929/1‑249 (MQ=255)                                                                                                                                                                    aaaaTACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTCAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAAt                                                               >  1:179186/1‑250 (MQ=255)                                                                                                                                                                        tACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCCAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGc                                                          >  1:23402/1‑251 (MQ=255)                                                                                                                                                                           gCAATGTTTCCATGCCGCGCAAGCGATCGAGGAAATGCCCACTTAAGCGAGGAAGAGCGAGAAAGTTACGTCGATTAGCATCGCAAGCCCCCAATCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAATATTGCCACCACAATCAGCAACAGAACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAAGCATGCata                                                       <  2:203989/251‑1 (MQ=255)                                                                                                                                                                              aTGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAGGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCg                                                    <  1:120189/251‑1 (MQ=255)                                                                                                                                                                               tGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGAATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGt                                                   >  1:274819/1‑251 (MQ=255)                                                                                                                                                                                gTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTc                                                  <  1:378206/251‑1 (MQ=255)                                                                                                                                                                                        gCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGc                                           <  1:393241/250‑1 (MQ=255)                                                                                                                                                                                        gCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCt                                          <  1:33560/251‑1 (MQ=255)                                                                                                                                                                                                   gATCGAGGAAATGCCCACTTAAGCGAGCACGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGc                               >  2:363371/1‑251 (MQ=255)                                                                                                                                                                                                   gATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGc                               <  2:265281/251‑1 (MQ=255)                                                                                                                                                                                                      cGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTc                            >  1:391795/1‑251 (MQ=255)                                                                                                                                                                                                              tGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCt                    >  1:306258/1‑251 (MQ=255)                                                                                                                                                                                                                       aaGCGAGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAgg           <  2:341210/251‑1 (MQ=255)                                                                                                                                                                                                                         gcgtgCAAGAGCGAGATAGTTACGGCGGTTAGCATCGTCAGCCACCATGCCAACGAGCGCCTTGAACAACGGAAAGAACTGTGCAGTGCACAGCAGACTGAGCGCCGCAGCCCAGTTAGAGGGTAAGGTTGCCAACACAAGCAGCAACGGCTCCGACCCTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCTTGCATATCGGCAATTGGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGtt         <  2:28859/247‑1 (MQ=255)                                                                                                                                                                                                                           aaGCAAGAGCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCAGTCCAACCAGCGCCATAAACACCGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTa       <  2:38080/250‑1 (MQ=255)                                                                                                                                                                                                                             gCAAGAGAGAGAAAGATAAGTCGATTAGCATCGGCAGCCCCCCTTCTAACCAGCGCCATAAACAACGAAATTAACGGTGCAGTGCCCAGCAGCATGAGCCCCGCAGCCCCGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCTAGACTGCCAGCGCCATTTGCGGCAGATCGCGTTCATCGCAATCATCCTTATCGACAAATTGCTCGAGTACCAGCGTTTCCCAGCTCCACGTAGGTTTAcc     >  1:395651/1‑251 (MQ=255)                                                                                                                                                                                                                                agagCGAGAAAGTTACGTCGATTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTg  <  1:117637/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       ATAGGAAAAACCAAAGTAGACCGCCACCAGAGCAATTGACAGCGAGGTAAAAAATTCGAGAATGCCGGAGGATAAAAACGCCAGCCGTAGCACTTCCATTGTCCGTTGGCGGAAATCTTCCGAAGCAGAACGAATACTTTCAATTTCAGCTTCACCACGACCAAAAATACGCAATGTTTCCATGCCGCGCAGGCGATCGAGGAAATGCCCACTTAAGCGAGCAAGAGCGAGAAAGTTACGTCGGTTAGCATCGGCAGCCCCCATTCCAACCAGCGCCATAAACAACGGAATTAACGGTGCAGTGCCCAGCAGAATGAGCGCCGCAGCCCAGTTAGAGGGGAAGATTGCCACCACAATCAGCAACGGCACCGACACTGCCAGCGCCATTTGCGGCAGATAGCGTGCATAGTAATCATGCATATCGTCAATTTGCTCGAGTACCAGCGTCGCCCAGCTCCCCGCAGGTTTACCCTG  >  NC_000913/930147‑930620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑