Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,841	G→A	P49S (CCT→TCT)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,841	0	G	A	100.0%	148.4 / NA	43	P49S (CCT→TCT)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/30);  total (13/30)

ACGCAGGTGAGCGACTTCACGCAGATACTCAATGCTGTGGCGTTTTGCCGCCATCGGGTAAGTGTCTGGATCTTCAACCCAACCAGCAACTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGCATCTG  >  NC_000913/988602‑989067                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     aCGCAGGTGAGCGACTTCACGCAGATACTCAATGCTGTGGCGTTTTGCCGCCATCGGGTAAGTGTCTGGATCTTCAACCCAACCAGCAACTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAgg                                                                                                                                                                                                                           <  1:338911/251‑1 (MQ=255)  cGCAGGTGAGCGACTTCACGCAGATACTCAATGCTGTGGCGTTTTGCCGCCATCGGGTAAGTGTCTGGATCTTCAACCCAACCAGCAACTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAgg                                                                                                                                                                                                                           >  2:141389/1‑250 (MQ=255)     aGGTGAGCGACTTCACGCAGATACTCAATGCTGTGGCGTTTTGCCGCCATCGGGTAAGTGTCTGGATCTTCAACCCAACCAGCAACTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAAcc                                                                                                                                                                                                                       >  2:228932/1‑251 (MQ=255)      ggTGATCGACTTCACGCAGATACTCAATGCTGTGTCGTTTTGCCGCCATCGGGTAAGTGTCTGGCTCTTCAACCCAACCAGCAACTTCAACCTTTCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGTCCGAGCAGCTGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGTGCATAGAAGTAGTGATGACAGCCTGTACAGAATCAAAGCAGGAACCg                                                                                                                                                                                                                      <  2:38595/251‑1 (MQ=255)                 tCACGCAGATACTCAATGCTGTGGCGTTTTGCCGCCATCGGGTAAGTGTCTGGATCTTCAACCCAACCAGCAACTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACgg                                                                                                                                                                                                            <  2:272807/250‑1 (MQ=255)                  cACGCAGATACTCAATTCTGTGGCGTTTTGCCGCCATCGGGTAAGTGTCTGGATCTTCAACCCAACCAGCAACTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCGGGAGCCGTCATAAACGGc                                                                                                                                                                                                           <  1:365310/250‑1 (MQ=255)                               aaTGCTGTGGCGTTTTGCCGCCATCGGGTAAGTGTCTGGATCTTCAACCCAACCAGCAACTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGc                                                                                                                                                                                             >  1:7113/1‑251 (MQ=255)                                            tttGCCGCCATCGGGTAAGTGTCTGGATCTTCAACCCAACCAGCAACTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAAt                                                                                                                                                                                 <  1:286814/250‑1 (MQ=255)                                                                    ggATCTTCAACCCAACCAGCAACTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATcc                                                                                                                                                           <  1:200061/248‑1 (MQ=255)                                                                      aTCTTCAACCCAACCAGCAACTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGCGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGCAACCATCCccgc                                                                                                                                                       >  1:179488/1‑250 (MQ=255)                                                                                 aaccAGCAACTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGAc                                                                                                                                               <  1:112321/247‑1 (MQ=255)                                                                                 aaccAGCAACTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGGAACCCTCCCCGCACgggggcaggt                                                                                                                                           >  2:112321/1‑243 (MQ=255)                                                                                         aCTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAAc                                                                                                                                   <  2:144418/251‑1 (MQ=255)                                                                                              aaCCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTCCCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATc                                                                                                                                                                                                                                   <  2:42366/150‑1 (MQ=255)                                                                                              aaCCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGCGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATc                                                                                                                                                                                                                                   >  1:42366/1‑150 (MQ=255)                                                                                                  ttGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCCTCCGCGCCCGGAGACTTCTCTGTCAACGGCTacacc                                                                                                                          >  1:104620/1‑250 (MQ=255)                                                                                                   tGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTacac                                                                                                                           <  2:117801/249‑1 (MQ=255)                                                                                                    gCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACgg                                                                                                                         <  1:228932/250‑1 (MQ=255)                                                                                                    gCTGGCCTAAATTTCAAATTGTTGCCCCTGGCCAGGCGACGCCACGCCTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGTCGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACgg                                                                                                                         <  2:208505/250‑1 (MQ=255)                                                                                                             aaTTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTa                                                                                                              <  1:210678/251‑1 (MQ=255)                                                                                                              aTTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAc                                                                                                             <  2:26189/251‑1 (MQ=255)                                                                                                              aTTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAc                                                                                                             <  1:30224/251‑1 (MQ=255)                                                                                                                        gttgCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACa                                                                                                  <  1:393175/251‑1 (MQ=255)                                                                                                                             cccTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGcac                                                                                             <  2:366852/251‑1 (MQ=255)                                                                                                                             cccTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGAACCCCTCCGCGCACGGAGACCTCGCTGGTAGCGGCTACCCGG‑ACCTCGCGTAC‑GGCGGCGACAgggac                                                                                             >  2:48867/1‑248 (MQ=255)                                                                                                                                    ccGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGGAACCATCCGCGCACGGTGACTTCGCTGTTAAAGGCCACACGG‑CCCTGGAGGACCGT‑GGCTACAGGCCCAACGCTc                                                                                      >  1:239846/1‑251 (MQ=255)                                                                                                                                       gCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGCACAACGCTCata                                                                                   <  1:241189/251‑1 (MQ=255)                                                                                                                                              cACGACTTTACCCGTCACAATGACCGGGCAGCCGGTGGTCAGACGCAGGACGTCGTCATTGTAAGTGGGCAGAGAATTATTGATGACAGCCGGTACAGAATCAAAGCAGGAACCGTCACAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGCACAACGCTCATAATATtct                                                                            <  2:41038/251‑1 (MQ=255)                                                                                                                                                                aaTGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGaa                                                          <  2:294200/251‑1 (MQ=255)                                                                                                                                                                     ccGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAAGTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAAt                                                     <  2:254127/251‑1 (MQ=255)                                                                                                                                                                      cGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCCTCCGCGCACGGGGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGGAC‑GTCGGCTACAGGCACAACGCTCCTAATATTTTCTCTGTTAATAGTCGGAAAAAAt                                                     >  1:131792/1‑250 (MQ=255)                                                                                                                                                                       gAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGGACGGAACCATCCGCGCCAGGTGGATTTGCTGTCAACGGCTACCCGG‑CCCCGGGGTAC‑GTCGGCTACAGGCACAACCCTCATAATATTTTCTCTGGTAATAATATGAAAAAATa                                                    >  1:76780/1‑250 (MQ=255)                                                                                                                                                                          cagcCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTc                                                                                                                                            <  1:48097/161‑1 (MQ=255)                                                                                                                                                                                gtggtCAGACGCAGGCCATCTTCAGTGTAATTGGGCAGCGAAGTACTGATGACAACCTGTACAGAATCAAAGCAGGAACCGTCATAACCGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCTCGCACCGAGACTTCGCTGTCAACGGCTACTCGG‑CCCTGTAGTAC‑GTGGGCTACAGTCAAAAAGCTAATAATATGCTCGCTCTTAAGAGTCGGAAAAAATAAACACTTGTc                                          <  2:310075/251‑1 (MQ=255)                                                                                                                                                                                     cAGACGCTGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCATGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCAcc                                      <  1:195736/250‑1 (MQ=255)                                                                                                                                                                                           cAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAAt                               <  1:262890/251‑1 (MQ=255)                                                                                                                                                                                                 gTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGGGACTTCGCTGTCAAAGGCTACACGG‑CCCCGGGGGAC‑GTGCGCTACAGGCACAACGCCCCTAATATTATCTCTGTTAATAGTCGGGAAAAATAAAAACTTGTCCACCCGTAAAAgggggg                         >  1:344578/1‑251 (MQ=255)                                                                                                                                                                                                   attCATTGTAAATTGGCAGAGAATTAATGTTGACTGCCTGCACAGAATCAAAGCAGGAGCCGGCATAAACGGCGAGGAAGGAGATTCCAACTTGTGACTCTCGGCTGGTACATCCCCATCCGCGCACGGTGACTTCGCTTTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGCACAACGCTCCTAATATTCGTTCTGTCAATAGTCGGAAAAAATAAACACGTGTCCCCCCGTATATgggggaa                        <  2:296519/249‑3 (MQ=255)                                                                                                                                                                                                      cATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCATGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTGTGAATCTCGGCGGGTTCGTACCCAGCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGCACAACGCTCATAATATTCTCTCGGTTAAGAGTCGGAAAAAATAAACACTTGTCCACGCGGAAATGGGGGGATAc                     <  2:179431/250‑1 (MQ=255)                                                                                                                                                                                                      cATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATAcc                    <  2:143393/251‑1 (MQ=255)                                                                                                                                                                                                           tAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGGACGGACCCATCCGCGCACGGTGACTTCTCTGTCAACGGCTACACGGGCCCTG‑AGTAC‑GTCGGCGACAGGGACAACACTCATAATATTCTTTCTGTTAATAGTCGGAAAAAATAAAAACTTTTTCACCCGTAAAAggggggggaactattt               >  2:163692/1‑241 (MQ=255)                                                                                                                                                                                                                   cAGAGAATTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGc       <  2:91414/251‑1 (MQ=255)                                                                                                                                                                                                                        aaTTATTGATGACAGCCTGTACAGAATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGCATCTg  <  2:7113/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     ACGCAGGTGAGCGACTTCACGCAGATACTCAATGCTGTGGCGTTTTGCCGCCATCGGGTAAGTGTCTGGATCTTCAACCCAACCAGCAACTTCAACCTTGCTGGCCTGAATTTCAAATTGTTGCCCCTGGCCCGGCGACGCCACGACTTTACCCGTCACAATGACCGAGCAGCCGGTGGTCAGACGCAGGACGTCTTCATTGTAATTGGGCAGAGAATTATTGATGACAGCCTGTACAGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGG‑CCCTGGAGTAC‑GTCGGCTACAGGCACAACGCTCATAATATTCTCTCTGTTAATAGTCGGAAAAAATAAACACTTGTCCACCCGTAAATGGGGGGATACCTATGTTACCTGGCATCTG  >  NC_000913/988602‑989067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑