Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,978,243	G→T	intergenic (‑46/+275)	flhD ← / ← insB5	DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,978,243	0	G	T	100.0%	73.8 / NA	23	intergenic (‑46/+275)	flhD/insB5	DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/12);  total (11/12)

ACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCGCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACT  >  NC_000913/1978003‑1978483                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 aCAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTg                                                                                                                                                                                                                                         >  1:269788/1‑250 (MQ=255)   aaaCCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATg                                                                                                                                                                                                                                      >  2:115039/1‑251 (MQ=255)                                      tGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCAtt                                                                                                                                                                                                   >  1:379481/1‑251 (MQ=255)                                        cGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGACGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAg                                                                                                                                                                                                 <  2:178189/251‑1 (MQ=255)                                                        gCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGc                                                                                                                                                                                 <  1:115039/251‑1 (MQ=255)                                                           aaCGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCt                                                                                                                                                                                <  2:382091/249‑1 (MQ=255)                                                                                     gCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGtt                                                                                                                                                     <  1:366522/250‑1 (MQ=255)                                                                                      ccGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGttt                                                                                                                                                    >  1:363990/1‑250 (MQ=255)                                                                                            cGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTAGTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTGGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGt                                                                                                                                             <  1:451622/251‑1 (MQ=255)                                                                                                                             aaaCGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATc                                                                                                            <  1:376555/251‑1 (MQ=255)                                                                                                                                  cTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTa                                                                                                          >  2:331722/1‑248 (MQ=255)                                                                                                                                                          gTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGt                                                                               >  1:97386/1‑251 (MQ=255)                                                                                                                                                                                 gCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACa                                                        <  2:363990/251‑1 (MQ=255)                                                                                                                                                                                      ccGGAGGTATGCATTATTCCCACCCAGAAGAACCAACTTTATTTATATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCTCCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGa                                                   <  2:93365/250‑1 (MQ=255)                                                                                                                                                                                          aGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAgggg                                               <  2:301848/251‑1 (MQ=255)                                                                                                                                                                                          aGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACGCGTCACTACACGCACATACAACGGAgggg                                               <  1:431597/251‑1 (MQ=255)                                                                                                                                                                                                          cacccaGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCaat                               >  1:223962/1‑251 (MQ=255)                                                                                                                                                                                                              caGAATAACCAACTTTATTTTTATGCGGTTTCCCCTCCCCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATCTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATg                           >  1:155387/1‑251 (MQ=255)                                                                                                                                                                                                                    aaCCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGAt                     >  2:201785/1‑251 (MQ=255)                                                                                                                                                                                                                     aCCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATg                    >  2:225723/1‑251 (MQ=255)                                                                                                                                                                                                                      ccAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGc                   >  1:84072/1‑251 (MQ=255)                                                                                                                                                                                                                                  ttATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACaa        <  1:201785/250‑1 (MQ=255)                                                                                                                                                                                                                                       cGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCTGTAAAAAATAATTAGTATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATTGGTCTTTACGCTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTTATGCAGATCACACAAAACACt  <  2:47655/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 ACAAACCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCGCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACT  >  NC_000913/1978003‑1978483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑