Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,129,008	C→T	V215I (GTC→ATC)	wcaE ←	GDP‑Fuc:2/3‑O‑Ac‑alpha‑L‑Fuc‑(1‑>3)‑alpha‑D‑Glc‑ PP‑Und alpha‑(1,4)‑fucosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,129,008	0	C	T	96.4%	90.3 / NA	28	V215I (GTC→ATC)	wcaE	GDP‑Fuc:2/3‑O‑Ac‑alpha‑L‑Fuc‑(1‑>3)‑alpha‑D‑Glc‑ PP‑Und alpha‑(1,4)‑fucosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (12/15);  total (13/15)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGACTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGAAAAATATCGCCTGATGACTGGCGGGCAGGCTGTGATAAATATACC  >  NC_000913/2128784‑2129241                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           cGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTcc                                                                                                                                                                                                                  >  1:336128/1‑250 (MQ=255)          aaaTATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATtgg                                                                                                                                                                                                        <  2:83683/251‑1 (MQ=255)              atTGTTGCCTGTAATGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTG‑ACTTTTTTTCGCGTCAGCACACAATTACATATTATtggtgtt                                                                                                                                                                                                    >  2:15714/1‑249 (MQ=255)                              cccACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATg                                                                                                                                                                                    >  1:257658/1‑251 (MQ=255)                              cccACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATg                                                                                                                                                                                    <  1:366178/251‑1 (MQ=255)                                              tttAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACAcc                                                                                                                                                                     >  2:75842/1‑250 (MQ=255)                                               ttAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCa                                                                                                                                                                    <  1:268864/250‑1 (MQ=255)                                                tAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAgg                                                                                                                                                                  >  1:289179/1‑251 (MQ=255)                                                      cGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCATCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGGAGATACCCCCCCCATGGAAAATTCAGACACCAGGCCATTg                                                                                                                                                            <  1:24518/251‑1 (MQ=255)                                                          gccgccCCGGAACCCGTTCGGCACCGAGAAACCGCTTAAAGCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGt                                                                                                                                                         <  2:302871/250‑1 (MQ=255)                                                           ccgccCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGttt                                                                                                                                                       <  2:336128/251‑1 (MQ=255)                                                            cgccCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTcc                                                                                                                                                                                                                  >  2:119976/1‑191 (MQ=255)                                                            cgccCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGGCAGCACACAATTcc                                                                                                                                                                                                                  <  1:119976/191‑1 (MQ=255)                                                            cgccCCGGAACCCATTAGGCACCGCGAAACCGCTTAAATCTTGCCTTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGtttt                                                                                                                                                      >  2:438869/1‑251 (MQ=255)                                                                           tCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAAcc                                                                                                                                        <  2:165553/250‑1 (MQ=255)                                                                                     gaAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACa                                                                                                                             <  2:289179/251‑1 (MQ=255)                                                                                             ttAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATACGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCt                                                                                                                     >  1:179118/1‑251 (MQ=255)                                                                                                     tGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAgc                                                                                                               <  1:309057/249‑1 (MQ=255)                                                                                                                tCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTCCGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAGCGCGTAGTCGga                                                                                                    >  1:200187/1‑249 (MQ=255)                                                                                                                             aCTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCCGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTc                                                                                     >  1:266005/1‑251 (MQ=255)                                                                                                                                        taAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATa                                                                                                                                                                                               <  1:172064/134‑1 (MQ=255)                                                                                                                                        taAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATa                                                                                                                                                                                               >  2:172064/1‑134 (MQ=255)                                                                                                                                             cTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑AGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCCGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCAt                                                                     >  2:383916/1‑251 (MQ=255)                                                                                                                                                                                     tAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCa                                                                                                                                                                    >  2:96362/1‑116 (MQ=255)                                                                                                                                                                                     tAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCa                                                                                                                                                                    <  1:96362/116‑1 (MQ=255)                                                                                                                                                                                       aTTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGAAAAATATCGTCTGATGACTg                           <  2:309568/251‑1 (MQ=255)                                                                                                                                                                                                      cAGGCACATGTAATATTTGTCGTTGGATTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCGCCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGAAAAATATCGCCTGATGACTGGCGGGCAGGCTGTGa            <  1:438869/251‑1 (MQ=255)                                                                                                                                                                                                                 aaTATTTGTCGTTGGATGTTTTT‑CGCGTCAGGACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACATCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATCCATTTTGGCTGCCAGCGCGTAGTCGTAAGAAACTTTATATGCCAGGTCATAACTCCATTTTTTCAAGCCGTATACAGGGAAAAATATCGCCTGATGACTGGCGGACAGGCTGTGATAAATATAcc  <  1:266089/250‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           CGACCAGGCAAATATTGTTGCCTGTACTGCCCACCATAATTGCACTTTAATAGCGTTGCCGCCCCGGAACCCTTTCGGCACCGAGAAACCGCTTAAATCTTGCATTGGTTTTCCTTATATTCAGACTTTGTTATATAAGGCTTTCGTCTTTGAGGTAGTACGTTGGCGTAAATGCCAGGATAATTCAGCCCAAAAGCCAGGCACATGTAATATTTGTCGTTGGACTTTTTT‑CGCGTCAGCACACAATTCCATATTATTGGTGGTAGATACCCCACCCATGGAAAATTCAGACACCAGGCCATTGAGTTTTTTAAATGCATAACCGGCTTTATACATTTTGGCTGCCAGCGCGTAGTCGGAAGAAACTTTATATTCCAGGTCATAACGCCATTTTTTCAAGCCGGATACAGGGAAAAATATCGCCTGATGACTGGCGGGCAGGCTGTGATAAATATACC  >  NC_000913/2128784‑2129241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑