Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,434,410	A→C	S100R (AGC→CGC)	ytfH →	putative transcriptional regulator YtfH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,434,410	0	A	C	100.0%	125.8 / NA	37	S100R (AGC→CGC)	ytfH	putative transcriptional regulator YtfH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/20);  total (17/20)

GCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGCCAATAAATGCCGGATGCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCA  >  NC_000913/4434171‑4434638                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     gCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGTGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGtt                                                                                                                                                                                                                           <  1:93032/251‑1 (MQ=255)     gTCGCGCGAGGTGGTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGGGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTtgctg                                                                                                                                                                                                                       <  2:166935/251‑1 (MQ=255)            gAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGc                                                                                                                                                                                                                <  1:339502/251‑1 (MQ=255)                 gtTGAAACACGTCAACAGCCGTTGGGGGGTGTTGATTCTGGTGGAGCTACGAGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACt                                                                                                                                                                                                           >  2:39767/1‑251 (MQ=255)                            tCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTg                                                                                                                                                                                                >  2:181096/1‑251 (MQ=255)                                cAGCCGTTGGGGGGTGTTGATTCGGGTTGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCGGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATTCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGAATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAAtt                                                                                                                                                                                            <  2:227295/251‑1 (MQ=255)                                            ggTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTGAGCGACATGAGGCGCAAAATCGGTGGGGTGAGTGAAAGGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATAGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGCCGAGCAGGTGCGCGTAAATGTTGCTGCACTGGCGGCCTGGGGTGAGTTGAATTTGccctctgtgt                                                                                                                                                                                >  2:130538/1‑244 (MQ=255)                                                  gATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCg                                                                                                                                                                           <  2:340687/250‑1 (MQ=255)                                                                 aagcgAAGGTACTCATCGCTTTAGCGACCTGAGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGACCGTg                                                                                                                                                           <  2:236981/249‑1 (MQ=255)                                                                      aaGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCa                                                                                                                                                      <  1:211689/251‑1 (MQ=255)                                                                              cATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGGGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTg                                                                                                                                                <  2:444765/249‑1 (MQ=255)                                                                                 cGCTTTAGCGACCTGCGGCGCAAAAACGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCgg                                                                                                                                            <  2:234796/250‑1 (MQ=255)                                                                                     ttAGCGACCTGCGGGGCAAAATCGGCGGGGTGAGTGAAAAGATGATTGCGCAGAAGTTACAGGCGTTAGAACAGGATGGCTTTCTTAAGCGGATCGCGCATCAGGTGGTGCAGCCGCATGTGGAATATAGCATCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCAGAGGTGATGGCGGTGCGGGATGAACGTGCGGCAAAAATGCCAGATgcc                                                                                                                                       >  2:66837/1‑250 (MQ=255)                                                                                       aGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCt                                                                                                                                     <  1:214814/251‑1 (MQ=255)                                                                                                   cgcAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTgg                                                                                                                         >  1:301863/1‑251 (MQ=255)                                                                                                     cAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTACCCGTATCGCGTAGCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTc                                                                                                                       <  2:317954/251‑1 (MQ=255)                                                                                                                           aaGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGCGCATGGCTTGTAGGGc                                                                                                 >  1:348528/1‑251 (MQ=255)                                                                                                                            agctGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGATTTCTTAACCCTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCCAGCAGGTGCGCGAAACGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGGACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCg                                                                                                >  1:46259/4‑251 (MQ=255)                                                                                                                            aGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGCGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCg                                                                                                >  1:46255/1‑251 (MQ=255)                                                                                                                                           cGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGAGCATGGCTTGTAGGGCGGATAAGATgctcc                                                                                   >  2:392052/1‑246 (MQ=255)                                                                                                                                                       tAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGc                                                                     >  1:98791/1‑251 (MQ=255)                                                                                                                                                        aGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGTGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGATTGTAGGGAGGATAAGGTGCGCAAGCACCGCATCCGcc                                                                    >  2:431313/1‑251 (MQ=255)                                                                                                                                                         gAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGCGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGccc                                                                   >  2:287409/1‑250 (MQ=255)                                                                                                                                                                  ggTTTTCTTACCCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGTATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGCCAATAAATGcc                                                          <  1:429913/251‑1 (MQ=255)                                                                                                                                                                           aaccttaccgcgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCGGCACATCCGCCAATAAATGCCGGATGCGGt                                                 <  1:122060/243‑1 (MQ=255)                                                                                                                                                                               gtaccgcgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGCCAATAAATGCCGGATGCGGTtgat                                             <  1:371792/247‑1 (MQ=255)                                                                                                                                                                                    ccgTATCCGTTGGTGCCGCGGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTGGCTGCACTGGCGGCCTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGGGCGGCAGAACTGCCGGATGCGCTGAGCTTTTTTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCACCATCGCATCCGCCAATAAATGCCGGATGCGGTTGATGAttt                                        <  1:436965/250‑1 (MQ=255)                                                                                                                                                                                      gTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGCCAATAAATGCCGGATGCGGTTGATGATTtat                                      >  1:49719/1‑251 (MQ=255)                                                                                                                                                                                          ccGGTGGTGCCGCCGCAGGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGTTCTGGCGGACTGGATTGAGTTTAATTGGCCCGAGGGTTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGATTTTATCTGGTCTACGTTGCATGGCTTGTAGGGCTTATAAGATGCGCCAGCATGGCTGCCGTCACTAAATGCCGGATGCGGTTGATGATTTATAGGc                                  <  1:174151/251‑1 (MQ=255)                                                                                                                                                                                          ccGGGGGTGCGGCGGCAGGTGGAATAGAGCCTCACGCCGCTGGGCGAGCAGGTGCGAGAAAAGGTGGCTGCACTGGCGGACTGGTTTGAGTTGAATTTGCCCGAGGGGTTGGCGGGGGGGGATGAAGGTGCGGTATAACTGCCGGATGGGCTGAGCTTATCTGGTCTCGGGTGCATGGTTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGCCAATAAATGCCGGATGCGGTTGATGATTTATAGGc                                  <  2:391183/251‑1 (MQ=255)                                                                                                                                                                                            ggtgttGCCCCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGCCAATAAATGCCGGATGCGGTTGATGATTTATAGGCCg                                <  1:433403/251‑1 (MQ=255)                                                                                                                                                                                            ggtggtGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGCCAATAAATGCCGGATGCGGTTGATGATTTATAGGCCg                                >  1:305535/1‑251 (MQ=255)                                                                                                                                                                                                  gccgccGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCCTCCGCCAATAAATGCCGGATGCGGTTGAACATTTATTGGCCGaaga                            >  2:12332/1‑247 (MQ=255)                                                                                                                                                                                                    cgccgcAGGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGTTTGCTGCACGGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGACCGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGCCAATAAATGCCGGATGCGGTTGATGATTTATAGGCCGAAAaagaa                        <  1:208454/251‑1 (MQ=255)                                                                                                                                                                                                             tgGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGCCAATAAATGCCGGATGCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTttac               >  2:120927/1‑251 (MQ=255)                                                                                                                                                                                                                        cTCACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGCCAATAAATGCCGGATGCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATc    >  2:42346/1‑251 (MQ=255)                                                                                                                                                                                                                          cACGCCGCTGGGCGAGCAGGTGCGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGTTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGCCAATAAATGCCGGATGCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCa  <  1:457587/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     GCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTGGCGCTACGCGAAGGTACTCATCGCTTTAGCGACCTGCGGCGCAAAATCGGTGGGGTGAGTGAAAAGATGCTTGCGCAGTCGTTACAGGCGTTAGAACAGGATGGTTTTCTTAACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGCTTGTAGGGCGGATAAGATGCGCCAGCATCGCATCCGCCAATAAATGCCGGATGCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCA  >  NC_000913/4434171‑4434638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑