Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	503,911	G→A	I262I (ATC→ATT)	fsr ←	fosmidomycin efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	503,911	0	G	A	95.7%	69.1 / ‑5.8	24	I262I (ATC→ATT)	fsr	fosmidomycin efflux pump
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (12/10);  total (14/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ACCAGAATGGCAGAGAATGCCGAAGCGAGGATAAATCCAATAATCACCGTTAAAACCCCC‑GTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGAAATATTTCGAGAAAATGAGGATTAACA‑GAATGCTGACCGCCAGTACAACTTTATTGCGCGGCAGTGGATTGATAATCGTCGCTTTGGGTTTTCCTTTATTCATTCGGTGCTGTGCCGAGTACCAACGGCTGATTTGCGCCAACACCACGATCGCCAGCAGTGCCGCAAGCACAAAC  >  NC_000913/503684‑504151                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  accagaatagccacgaATGACGAAGCGAGGAGAAATCAAATAAACACAGTTAAAACCCCC‑GTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGAGTAAACGGCGCAACGCCGAGGCTATAGCACCAAATCACACGTTCCCGACCAAATTTATCCCCGACAGGCCCGCCGATCACCATAACTGCCGACACGGAAAACAGGAAAGCAAACAGAGGAAGCGGAGCATTCTGCATAGATAAGCCGAATTTTTgact                                                                                                                                                                                                                            >  2:323926/15‑248 (MQ=255)  caaaaagggcaaaaaaTGCCGAAGCGAGGAAAAACCCAATAACCCCCGTAAAAACCCCC‑GTCAAGGGCGGGCTGGCGTGGGGAAAAAACAGCGAAAACGGCGAACCGCCGAGGATAGACCCCAAAATCACATATTCCCCCCCAATTTAACCCCATACAGGCCCGCCGATCCCCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATc                                                                                                                                                                                                                           <  1:255464/237‑1 (MQ=255)   aaaaaTGGCAGAAAATGCCGAACATGGGAAAAATCCAATAATCACGGTTAAAACCCCC‑GTCCAGTCCGGGCTGGTGTAGGGAAAAATCAGCTTAACCGGCGCAACGCGGGGGATAAAGCCCCAAATCACATATTCCCCCCAAATTTTATCCCCTACAGGCCCGCCGATCACCGTTCCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCa                                                                                                                                                                                                                          <  1:327337/248‑1 (MQ=255)      aagggCAGAGAATGCCGAAGGGAGGAAAAACCCAAAAATCACCGTAAAACCCCCC‑GTCCAGTGCAGGCGGGCGTGGGGAAAAATCAGCGAAAACGCCGCACCGCCGAGAAAAGACCCCAAAACCCCATATTCCCCCCCAATTTTACCCCCAACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGAt                                                                                                                                                                                                                       <  1:154867/248‑1 (MQ=255)                                 aaaTCCAATAATCACCGTTAAAACGCCA‑GTCCAGTGCCGGCTGTCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCCAACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAACCAGATGAAGCTGAGCATTCTGAATAGATAATCCGACTTTTTGCATCAGATAAAAGGTGGAATAACTGCTGATGCTCg                                                                                                                                                                                            >  2:159727/1‑251 (MQ=255)                                 aaaTCCAATAATCACCGTTAAAACCCCC‑GTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCg                                                                                                                                                                                            >  1:105287/1‑251 (MQ=255)                                           atCACCGTTAAAACCCCC‑GTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCTTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGa                                                                                                                                                                                   >  1:242185/1‑250 (MQ=255)                                           acccccGTCAAACCCCCC‑GTCCAGTGCAGGCTGGGGTAGGGAAAAACAAGCTAAAACGGCGCAACGCGGAGGAAAGACCCCCAAGTCAAATTTTCCCGCCCAATTTAATCCCCAAAAGCCCCTCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGaa                                                                                                                                                                                  <  2:375345/247‑1 (MQ=255)                                                 gTTAAAACCCCC‑GTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGAAATAttt                                                                                                                                                                            >  2:279352/1‑251 (MQ=255)                                                  atAAAACCCCCCGCCAAGTGCAGGCGGGCTTGGGGAAAAATCAGCGTAACCGGCCCAACGCCGAGAATAGACCCCAAAATCAAATATTCCCGCCCAATTTTACCCCCAACAGCCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGAAATAttt                                                                                                                                                                            <  1:88376/250‑1 (MQ=255)                                                                                                                  ggATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGAAATATTTCGAGAAAATGAGGATTAACA‑GAATGCTGACCGCCAGTACAACTTTATTGCGCGGCAGTGGATTg                                                                                                           >  1:406395/1‑251 (MQ=255)                                                                                                                    aTAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGAAATATTTCGAGAAAATGAGGATTAACA‑GAATGCTGACCGCCAGTACAACTTTATTGCGCGGCAGTGGATTGAt                                                                                                         >  1:236489/1‑251 (MQ=255)                                                                                                                                   acaTATTTCCGCCCAATTTTATCCCCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGAAATATTTCGAGAAAATGAGGATTAACA‑GAATGCTGACCGCCAGTACAACTTTATTGCGCGGCAGTGGa                                                                                                              >  1:66763/1‑231 (MQ=255)                                                                                                                                              cccAATTTTATCCCCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAATCAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGAAATATTTCGAGAAAATGAGGATTAACA‑GAATGCTGACCGCCAGTACAACTTTATTGCGCGGCAGTGGATTGATAATCGTCGCTTTGGGTTTTCCTTTat                                                                               >  1:188105/1‑251 (MQ=255)                                                                                                                                                              aCAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGAAATATTTCGAGAAAATGAGGATTAACA‑GAATGCTGACCGCCAGTACAACTTTATTGCGCGGCAGTGGATTGATAATCGTCGCTTTGGGTTTTCCTTTATTCATTCGGTGCTGTGc                                                               >  1:234159/1‑251 (MQ=255)                                                                                                                                                                            ccccttcccTGCCGCAACGGCAAACAGGAAGGAAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTAATGCTCGCCATATAGAAATTTTTCGAGAAAATGAGGATTAACA‑GAATGCTGACCGCCAGTACAACTTTATTGCGCGGCAGTGGATTGATAATCGTCGCTTTGGGTTTTCCTTTATTCATTCGGTGCTGTGCCGAGTACCAACGGc                                                 <  1:398319/244‑1 (MQ=255)                                                                                                                                                                              ccGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGAAATATTTCGAGAAAATGAGGATTAACA‑GAATGCTGACCGCCAGTACAACTTTATTGCGCGGCAGTGGATTGATAATCGTCGCTTTGGGTTTTCCTTTATTCATTCGGTGCTGTGCCGAGTACCAACGGCTg                                               >  2:8923/1‑251 (MQ=255)                                                                                                                                                                                  aCCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGAAATATTTCGAGAAAATGAGGATTAACA‑GAATGCTGACCGCCAGTACAACTTTATTGCGCGGCAGTGGATTGATAATCGTCGCTTTGGGTTTTCCTTTATTCATTCGGTGCTGTGCCGAGTACCAACGGCTGAttt                                           >  1:77617/1‑251 (MQ=255)                                                                                                                                                                                             cGGCAAACAGGAGGGCAACCAGATGAAACGTAAAATTCTTAATGGATATTCCGAATTTGTGAATCAGATAAAAGGTGAAATAGTTGCTGATCCTCACATTAAAGAAATTTTTCAAGAAATTGAGGAT‑GACATGAATGCTGTCCGCCAGTACAACTTTATTGCGCTGCAGTGGATTGATAATCGTCGCTTTGGGTTTTCCTTTATTCATTCGGTGCTGTTCCTAGTACCAACGGCTGATTTGCGCCAAcacc                                <  2:131245/251‑1 (MQ=255)                                                                                                                                                                                                cAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGAATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGAAATATTTCGAGAAAATGAGGATTAACA‑GAATGCTGACCGCCAGTACAACTTTATTGCGCGGCAGTGGATTGATAATCGTCGCTTTGGGTTTTCCTTTATTCATTCGGTGCTGTGCCGAGTACCAACGGCTGATTTGCGCCAACACCACg                             >  2:61180/1‑251 (MQ=255)                                                                                                                                                                                                             aaaCAGCTGAAGCTGAGCATTCTCGATAGATAATCCGAATTTTTGCATCAGATAAAAGGAGTAATAGCTGCTGATGCTCGCCATATCGAAATATTTCGAGAAAATGAGGATTAACA‑GAATGCTGACCGCCAGTACAACTTTATTGCGCGGCAGTGGATTGATAATCGTCGCTTTGGGTTTTCCTTTATTCATTCGGTGCTGTGCCGAGTACCAACGGCTGATTTGCGTCAACACCACGATCGCCAGCAGTg                >  2:37671/1‑251 (MQ=255)                                                                                                                                                                                                                aagaagAAGCTGAGCATTCAGAATAGATAATCCAAATTTTTGCATCAGATAAAAGGGGTAATAGCGGTGGATGCTCGCCATATAGAAATATTTCGAGAAAATGAGGATTAACA‑GAATGCGGACCGCCAGTACAACTTTATTGCGCGGCAGTGGATTGATAATCGTCGCTTTGGGTTTTCCTTTATTCATTCGGTGCTGTGCCGAGTACCAACGGCTGATTTGCGCCAACACCACGATCGCCAGCAGTGCCg             <  2:77617/246‑1 (MQ=255)                                                                                                                                                                                                                           gAGCATTTTGAATAGATAATCCGATCTTTTGCATCATATAAAAGGTGTAATAGCTGCTGATGCTCGCAATATAAAAATATTTCGAGAAAAGGAGGATTAAGA‑GAATCATGACCGCCAGTACAACTTTATTGCGCGGCAGTGGATTGATAATCGTCGCTTTGGGTTTTCCTTTATTCATTCGGTTCTGTGCCGAGTACCAACGGCTGATTTGCGCCATCACCACGATCGCCAGCAGTGCCGCAAGcacatac  <  1:334144/251‑4 (MQ=255)                                                                                                                                                                                                                           gAGCATTCGGAATAAATAACCCGAATTTTGGCTTCAGAAAAAAGGTGAAATATCGTCTTAGGCTCCCCATAGGGAAATATTTCGAGAAAATGAGGATTAACA‑GAATTCTGACCGCCAGTACAACTTTATTGCGCGGCAGTTGATTGAGAATCGTCGCTTTGGGTTTTCCTTTATTCATTCGGTGCTGTGCCGAGTACCAACGGCTGAGTTGCGCCAACACCACGATCGCCAGCAGTGCCGCAAGCACaa    <  1:17851/249‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  ACCAGAATGGCAGAGAATGCCGAAGCGAGGATAAATCCAATAATCACCGTTAAAACCCCC‑GTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTGATGCTCGCCATATAGAAATATTTCGAGAAAATGAGGATTAACA‑GAATGCTGACCGCCAGTACAACTTTATTGCGCGGCAGTGGATTGATAATCGTCGCTTTGGGTTTTCCTTTATTCATTCGGTGCTGTGCCGAGTACCAACGGCTGATTTGCGCCAACACCACGATCGCCAGCAGTGCCGCAAGCACAAAC  >  NC_000913/503684‑504151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑