Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	618,034	G→A	R1293K (AGG→AAG)	entF →	apo‑serine activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	618,034	0	G	A	100.0%	72.9 / NA	23	R1293K (AGG→AAG)	entF	apo‑serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/12);  total (11/12)

GCAACTACGCTGATGCTGTGCGCCTGCTGACGACTGCTCATAGCGTACCGTTTGACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACT  >  NC_000913/617800‑618264                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                       gCAACTACGCTGATGCTGTGCGCCTGCTGACGACTGCTCATAGCGTACCGTTTGACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATattatt                                                                                                                                                                                                                        <  1:254666/251‑1 (MQ=255)    aCTACGCTGATGCTGTGCGCCTGCTGACGACTGCTCATAGCGTACCGTTTGACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATAttatttat                                                                                                                                                                                                                     >  1:346937/1‑251 (MQ=255)      tACGCTGATGCTGTGCGCCTGCTGACGACTGCTCATAGCGTACCGTTTGACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGGATGAGTCCCGTACGCGCCTGGTCGACGTGGATAGCGGAGCTGGTTCTCTATCGCCAGGATTGTGCGCATGAGGATATTATCTCTCCAGGGACGTTTGAAACAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTtata                                                                                                                                                                                                                    >  2:364160/1‑250 (MQ=255)                             tacgacTGCTCATAGCGTACCGTTTGACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAaataa                                                                                                                                                                                              <  2:202736/248‑1 (MQ=255)                             gacgacTGCTCATAGCGTACCGTTTGACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAaataat                                                                                                                                                                                             <  1:151359/250‑1 (MQ=255)                                                 catttGACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGCCGTTTGAAAAAATTTGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTAtttgtttg                                                                                                                                                                        <  1:17580/249‑1 (MQ=255)                                                  gTTTGACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTAtttgtttg                                                                                                                                                                        >  2:342851/1‑250 (MQ=255)                                                      gACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGAttt                                                                                                                                                                    <  2:381228/250‑1 (MQ=255)                                                                             tgcggaatgCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGGGCGCATGTGGATATTATCTCTCCAGGGACGTGTGAAAAAAGTGGGCCGAGTATTCGCGCAACGCTAAACAAGTAAAGTAATATTATTTATAAACCCATAATTCCAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTctct                                                                                                                                             <  1:310803/242‑1 (MQ=255)                                                                                  aaCGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTAAAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAAt                                                                                                                                        >  1:168329/1‑250 (MQ=255)                                                                                                         aGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTgga                                                                                                                <  1:149876/251‑1 (MQ=255)                                                                                                                  cgcnCCTGGTCGCCGTAGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATAGTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGGGGAAGGAATTTa                                                                                                       <  2:416413/251‑1 (MQ=255)                                                                                                                               cGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCtt                                                                                          >  1:373025/1‑251 (MQ=255)                                                                                                                                                   atctatcGTCCGGGTTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGACAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAAAAACTTCATCTTCGGATACTTATCACCANCTg                                                                      >  2:410792/1‑251 (MQ=255)                                                                                                                                                            cAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTg                                                              <  2:436060/250‑1 (MQ=255)                                                                                                                                                                    atcgaatttgGATATTATCTCTACAGGGACGTTTGAAAAAATTGGGCCGATTTTTCGCGCAACGCTAAACAAGTAAATTATTTTTATTTATAAACGCATAATTACAGACAATAATTATGGGTTTTTTCTTTGCTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAATGCATTTACAAATTTCAGCTTCGGTTAATTATCAGCAACTGGTAAAGTGAGCGACGTg                                                     >  2:78223/9‑251 (MQ=255)                                                                                                                                                                         aTGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTgg                                                    >  1:360555/1‑247 (MQ=255)                                                                                                                                                                                           caaggACGTTTGAAAAAATTGGGCCTATTATTCGCGCAACGCTAAACAAGTAAAGTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGGTTGATTTATAGGGTTGATGAATATTTCTCTGAAATATAGTGAATGTTGCATGCAGTTGAGGGAAGTTACAAACTTCAGCTTCGGATAATTATCACCAACTCGTAAAGTGAGCGCCGTGGAGATTTCTCCCTATTGGACTCa                              <  2:433366/248‑1 (MQ=255)                                                                                                                                                                                              ggACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAGCTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCAttt                           <  1:342851/251‑1 (MQ=255)                                                                                                                                                                                                     tGAAAAGATTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGtt                    >  2:202495/1‑251 (MQ=255)                                                                                                                                                                                                        aaaaaTTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATCGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGatat                  >  1:50533/1‑250 (MQ=255)                                                                                                                                                                                                            aTTGGGCCGATTATTCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATCATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCCGGTTCTAcccc             >  1:37619/1‑251 (MQ=255)                                                                                                                                                                                                                        attCGCGCAACGCTAAACAAGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACt  <  1:405429/250‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                       GCAACTACGCTGATGCTGTGCGCCTGCTGACGACTGCTCATAGCGTACCGTTTGACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTGAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATTAATATTATTTATAAACCCATAATTACAGAAAATAATTATGGGTTTTTTATTTGTTTGATTTATAGGTTTGATGAATATTTCTCTTAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACT  >  NC_000913/617800‑618264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑