Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,397,444	G→A	Q908* (CAA→TAA)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,397,444	0	G	A	100.0%	241.3 / NA	72	Q908* (CAA→TAA)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/34);  total (38/34)

ATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCA  >  NC_000913/2397198‑2397685                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  aTGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATtattgt                                                                                                                                                                                                                                               >  1:395432/1‑251 (MQ=255)      gcgATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGcctc                                                                                                                                                                                                                                          >  1:59456/1‑251 (MQ=255)       cgATCCGAGAATTTCGGGATCCAGTCTTCTGTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGGGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGcctcc                                                                                                                                                                                                                                         >  1:399673/1‑251 (MQ=255)           ccGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGGCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAAAGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGACAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGAGCCTCCTTg                                                                                                                                                                                                                                      >  2:244085/1‑250 (MQ=255)             gagaATTTCGGGTTCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTgaga                                                                                                                                                                                                                                   <  2:393933/251‑1 (MQ=255)                aaTTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATcc                                                                                                                                                                                                                                <  1:428503/251‑1 (MQ=255)                       ggATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCa                                                                                                                                                                                                                             >  1:367793/1‑247 (MQ=255)                         aTCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATgc                                                                                                                                                                                                                       >  2:356619/1‑251 (MQ=255)                           ccAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATgcgc                                                                                                                                                                                                                     >  1:25091/1‑251 (MQ=255)                               tcttctTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCgcc                                                                                                                                                                                                                  >  1:457885/1‑250 (MQ=255)                                 ttcttTAAAGACAATTTTGATCATGTCCGAAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCgccag                                                                                                                                                                                                                <  2:152746/250‑1 (MQ=255)                                  tcttTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCgccagc                                                                                                                                                                                                               <  1:356619/250‑1 (MQ=255)                                    tttAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCCCCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCCGTTCCGGAGATATCCAACTCATTATTGTGCCTCCTTTAGATCCTCAAGATGCGCgccagcc                                                                                                                                                                                                              >  2:159021/1‑249 (MQ=255)                                     ttAAAGAGCAGTGTGATGATGTCCGCAACCAGCTGGAGAAACCCGCCCCAGCCAACACGGGTAGGTCCGTAACGGTGCTGGAACAGACCCAGCAGGCTACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGATAGCCTAAAGATGCGCgccagcca                                                                                                                                                                                                             <  2:342590/249‑1 (MQ=255)                                        aaGAACATTTTGATCAGGTCCGCAACCATCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCAcc                                                                                                                                                                                                        <  2:273426/251‑1 (MQ=255)                                           aaCATTTTGATCATGTACGCAACCAGCTGAAGCGAACCGCCCCAGACAACACAGTTAGGTACGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAACTCATGAATGCCCCGCAGGTAACAACCAACAGCAGGATCACCACAGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGCTGATATCCAACTCATTATTGTGCCTCCTTGAGAGCCTCAAGATGAGAGCCAGCAAGCACCgg                                                                                                                                                                                                      >  2:458096/1‑250 (MQ=255)                                                tttGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCaa                                                                                                                                                                                                 <  1:188028/250‑1 (MQ=255)                                                 ttGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGCGCAATg                                                                                                                                                                                               >  1:243096/1‑251 (MQ=255)                                                     tCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCgg                                                                                                                                                                                           >  2:242498/1‑251 (MQ=255)                                                               aaacagctggattaaaCCGCCCCAGCCAACACAGTTAGGTCCTTAACGGTTCTGGACCAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCTCAGGTTACAACCACCAGCAGGATCACCACCGCTTTGAGGATGTTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGAGCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCAt                                                                                                                                                                                 <  2:245392/237‑1 (MQ=255)                                                                 ccagctggcggaaaacGCCCCAGCCAACACAGTTAGGTCCGTAACGGTACTGGCACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCCCCACCGCTTTGAGGCTGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACGCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCACCAGCCAGCACCGGAGCAATGCCGGACATGCCCATCg                                                                                                                                                                               <  2:394821/236‑1 (MQ=255)                                                                   aGCTGGAGCGCAACGCCCCAGCCAAACCGGTTAGGTACGTCACGGTCCTGGAACAGACCCAGCAGACGACGTTCGATAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGACAGCAGGCTCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCACTGCCGGACATACCCATCGGc                                                                                                                                                                             >  2:199909/1‑251 (MQ=255)                                                                    ttgggagtgagccGCACCAGCCAACACGGTTATGTCAGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCAGGAAGGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCATTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGAGGCGCGCCAGCCAGCACCTGAGCAATCCCGGCCATACCCATCGGc                                                                                                                                                                             <  2:69837/239‑1 (MQ=255)                                                                     cTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGAGACAACCACCAGCAGGATCACCAACGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGCGCAATGCCGGACATACCCATCGGCa                                                                                                                                                                            >  1:263591/1‑250 (MQ=255)                                                                             aaCCGCCCCAGCCAACACGTCTAGTTCCGTAACGGTTCTGGAACAGACCCAGCATGCTAAGTTCGCCAAAGCTCATGAATGCCCCGCAGGGGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCATGATCTCAATCATTACCCGTTACATCCAACTCATTATTGTGCCTCCTTGAGACCCTCAAGAGGCGCGCCAGCCCGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAcct                                                                                                                                                                   <  2:91506/251‑1 (MQ=255)                                                                                    ccAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGCGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAcctgccctgc                                                                                                                                                            >  2:23697/1‑251 (MQ=255)                                                                                          aaCACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTc                                                                                                                                                        >  2:334547/1‑249 (MQ=255)                                                                                           acacGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGt                                                                                                                                                     >  1:207947/1‑251 (MQ=255)                                                                                                gTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTc                                                                                                                                                <  1:212884/251‑1 (MQ=255)                                                                                                   aGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGTTTATATCCAACTCATTATTGTGCCTCCGTGAGATCCTCAAGATGCTCGCCAGCCAGCACCGGAGTAATGCCGGGCATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTTCTTCGGc                                                                                                                                             <  1:412763/251‑1 (MQ=255)                                                                                                        cGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCACGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGAttt                                                                                                                                        >  1:198672/1‑251 (MQ=255)                                                                                                        cGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGAttt                                                                                                                                        <  2:270488/251‑1 (MQ=255)                                                                                                        cGTAACGGTTCTGGAACAGACCCAGCAGGAGACGTTCGCCAAAGCTCATGAATGCCCCGAAGGAGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTAAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATAATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACTCATAGGCAAGCCCACCTGCCCTGCCGTCACTCCTTCggagcttt                                                                                                                                        >  1:264165/1‑245 (MQ=255)                                                                                                            aCGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAAc                                                                                                                                    >  1:154208/1‑251 (MQ=255)                                                                                                              ggTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCg                                                                                                                                  <  1:32581/251‑1 (MQ=255)                                                                                                                 tCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATTCCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGc                                                                                                                                <  1:425359/250‑1 (MQ=255)                                                                                                                    ggAATAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGTTGACAACCTCCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTTCCTCCTTGATATCCTCAAGATGCGCGACAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCTCCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAg                                                                                                                              <  1:458096/249‑1 (MQ=255)                                                                                                                     gAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGCATGCCCCGCAGGTGACAACCACCCGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCACGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGt                                                                                                                           >  2:28139/1‑251 (MQ=255)                                                                                                                     gAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGTATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGt                                                                                                                           <  1:15039/251‑1 (MQ=255)                                                                                                                         aGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGAcc                                                                                                                       >  1:120477/1‑251 (MQ=255)                                                                                                                                 caagCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCCACCGGCAGCGTGACCGCGTTGcc                                                                                                               >  1:263970/4‑251 (MQ=255)                                                                                                                                    gggtcgtgcGCCAAAGCTCATGAATGGTCCGCAGGTGACAACCCCCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGTATCTCAATCAGTCCCGGTGATATCGAACTCATTATTGTGCCACTTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATTCCTGACATACCCAGCGGCAAGCCCACCAGCCCTGCCGCCAGCACTTCGGCGATTTCAACCGGCAGCGTGAACGTGTTGCCATc                                                                                                            <  2:234158/243‑1 (MQ=255)                                                                                                                                          ttCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACt                                                                                                      >  1:320040/1‑251 (MQ=255)                                                                                                                                            cGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTa                                                                                                     >  2:81470/1‑250 (MQ=255)                                                                                                                                                          aaTGCCCCGCAGGTGACAACCACCAGCAGGATCTCCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTa                                                                                      <  1:159021/251‑1 (MQ=255)                                                                                                                                                            tGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGAAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTAcc                                                                                    >  1:52227/1‑251 (MQ=255)                                                                                                                                                             gCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCt                                                                                   <  2:233172/251‑1 (MQ=255)                                                                                                                                                             gCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTAGTGTGCCTCCGTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAATGAGACGCGTGTACCt                                                                                   <  2:184039/251‑1 (MQ=255)                                                                                                                                                               cccGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGc                                                                                 >  1:286762/1‑251 (MQ=255)                                                                                                                                                                  gCAGGTGACAAACACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGtt                                                                              <  2:265641/251‑1 (MQ=255)                                                                                                                                                                    aggagagaaCCACCAGCAGGATCACCAGCGAGTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCAAACTCATTATTGTGCCTCCTTGAGATCCTCAAGAGGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGGAAGCCAACCAGCACTGCCGTGAGTCACTCGGCGATTTCAACCGGCAGGGGGACCGTGTTGCCATAGTAACTAAAGGAGGCGCGTGGACCGGCGTTc                                                                             >  2:297225/8‑250 (MQ=255)                                                                                                                                                                     ggTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTcac                                                                           >  1:84676/1‑251 (MQ=255)                                                                                                                                                                               accaGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTc                                                                 <  2:441492/251‑1 (MQ=255)                                                                                                                                                                                      ggATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCAt                                                          >  2:33521/1‑251 (MQ=255)                                                                                                                                                                                        aTCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATcc                                                        >  2:348763/1‑251 (MQ=255)                                                                                                                                                                                         tCACCACCGATTTGAGGATGGTACGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGCCATACCCATCGGCAAGCCCACCTGCCCTGCCGCCAGCCCTTCGGCGCTTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCg                                                       >  2:289621/1‑251 (MQ=255)                                                                                                                                                                                               ccGCGTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGTAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGt                                                 <  1:40830/251‑1 (MQ=255)                                                                                                                                                                                                   tttGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTg                                               >  1:88911/1‑249 (MQ=255)                                                                                                                                                                                                          aTGGGCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCACAAGAGGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCCCCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGTCCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTGTGATg                                      <  2:219656/251‑1 (MQ=255)                                                                                                                                                                                                               cancacgacctcAATCTGTTCCGGTTGTATCCAACGCACTATTGTGCCTCCTTGAGATCCTCAAGATGCCCGGCAGCCAGGACCGGAACAATGCCGGCCATAACCATCGGCACGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGGGAGCGTGTTACCATCGTAACTAAAGGATACGTGTGTCCCTGCGTATACCCCCAACTTCGCGGCTTCCGCTTGGTTTAGTGTGATGTACg                                  <  2:405182/241‑1 (MQ=255)                                                                                                                                                                                                               cagcagGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGACCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCGGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACGTCGCGGCATCCGCTGGGTTGAGTTTTATGTACg                                  <  1:312659/250‑1 (MQ=255)                                                                                                                                                                                                                 gcagGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCt                               >  2:301236/1‑251 (MQ=255)                                                                                                                                                                                                                          cAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCa                         <  1:33521/248‑1 (MQ=255)                                                                                                                                                                                                                          cAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCa                         <  1:346282/248‑1 (MQ=255)                                                                                                                                                                                                                          cAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATAc                      <  2:221730/251‑1 (MQ=255)                                                                                                                                                                                                                            aTCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACgg                    >  1:453673/1‑251 (MQ=255)                                                                                                                                                                                                                                   ccGGTGATATCTAACTCAATATTGTGCCCCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCGCCGGAGCAATGCCGGACATACCCAGCGGCAAACCCACCTGCCCTGCCGTCAGTCCTGCGGCGATTTCAACCGGCAGCGTGACCGTGTTACCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGGTGTACGGCTGCGGCATATGGCTCTgg              <  2:101945/250‑1 (MQ=255)                                                                                                                                                                                                                                     ggTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGa             >  1:3271/1‑249 (MQ=255)                                                                                                                                                                                                                                      gTGATATCCAACGCATTATTGGGCCGCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATCCCCAGCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGAGTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGGACCTGCGTTCACACCCAACTGCGCGGCAGCCGCTGGGTTGAGTTTGATTTACGGGTGCGGCATACGCCTCCGGAAGa          <  2:198672/251‑1 (MQ=255)                                                                                                                                                                                                                                        gATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGAcc        >  1:69529/1‑251 (MQ=255)                                                                                                                                                                                                                                             ccAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGCTTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGCTGTACGGCTGCGGCATACGGCTCTGGCAGACCGGAGc   >  1:298918/1‑251 (MQ=255)                                                                                                                                                                                                                                              cAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGc   >  1:232105/1‑250 (MQ=255)                                                                                                                                                                                                                                              cAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCa  <  2:207947/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  ATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGCA  >  NC_000913/2397198‑2397685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑