Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	474,597	G→A	P189S (CCG→TCG)	tesB ←	acyl‑CoA thioesterase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	474,597	0	G	A	100.0%	161.0 / NA	47	P189S (CCG→TCG)	tesB	acyl‑CoA thioesterase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/27);  total (20/27)

CGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAACCCGCTTCTGGTGCC  >  NC_000913/474351‑474835                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               cGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACg                                                                                                                                                                                                                                            <  1:90774/251‑1 (MQ=255)  gCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGc                                                                                                                                                                                                                                           >  2:137492/1‑251 (MQ=255)    cGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCt                                                                                                                                                                                                                                          >  2:258842/1‑250 (MQ=255)      tCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGAGATCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAGTGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGGCATCCGACACGCTAcc                                                                                                                                                                                                                                       >  1:423054/1‑251 (MQ=255)                  aaCTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGAt                                                                                                                                                                                                                           >  1:214857/1‑251 (MQ=255)                           cgcACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGACGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATACACACCTg                                                                                                                                                                                                                  >  2:2621/1‑251 (MQ=255)                           cgcACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTg                                                                                                                                                                                                                  <  2:342716/251‑1 (MQ=255)                                       cGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTc                                                                                                                                                                                                      <  1:178749/251‑1 (MQ=255)                                            gcTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGCACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCCGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACACTCCCCTTTGCGCGGATCCACACCTGACGATGGGGTTCTGCg                                                                                                                                                                                                  >  2:51642/1‑250 (MQ=255)                                               ggACGCCGAGGTGCGCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGAGGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGAc                                                                                                                                                                                                <  1:143632/249‑1 (MQ=255)                                                      gAGNTGCTCTACAAGCTATACAGCAGCAATTCATTCAAATTAAACGGGCGATGGAACAACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCGGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACGCGCTACCAGTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGGCCttt                                                                                                                                                                                       <  2:417248/251‑1 (MQ=255)                                                         gTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAg                                                                                                                                                                                    <  1:258842/251‑1 (MQ=255)                                                                       ataCAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGAGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGAGTAACCGAGCAGATACTGTTGAACGCGCAGGTAATCCGACACGCAACCATTTGCGCGGATCCACAACTGAAGATGAGGTTCTGAGACGTGACCTTTCAGTGGGTTATGACACt                                                                                                                                                                      >  2:96073/1‑251 (MQ=255)                                                                             cagcCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACcg                                                                                                                                                                >  1:85933/1‑251 (MQ=255)                                                                                         caAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAATTTAAGACCAGAAGCGTAACCGAGCAGATACTGATGAGCGCGCAGGTCACCCGACACTCTACCAGTTGCGCGGATCCACACCTGACGATGTGTTTCTGCGCCGTGGCCTTTCAGTGGTTTATGAAACTCCACCGGACGGACTTCCa                                                                                                                                                    <  2:423054/251‑1 (MQ=255)                                                                                           aaTTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGc                                                                                                                                                  >  1:100165/1‑251 (MQ=255)                                                                                            aTTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCACTCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGACGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGGCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCg                                                                                                                                                 >  1:258193/1‑251 (MQ=255)                                                                                                aaCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCCGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGCCGATGTGGTTCTGCGCCGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGNACg                                                                                                                                             >  2:418679/1‑251 (MQ=255)                                                                                                  cGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGAt                                                                                                                                           <  2:276080/251‑1 (MQ=255)                                                                                                  cGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGAt                                                                                                                                           >  1:25369/1‑251 (MQ=255)                                                                                                   gggCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATc                                                                                                                                          <  2:73987/251‑1 (MQ=255)                                                                                                          ggAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGAt                                                                                                                                    <  1:58433/250‑1 (MQ=255)                                                                                                          ggAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGAGCGCAGAt                                                                                                                                    <  1:58681/250‑1 (MQ=255)                                                                                                           gAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGa                                                                                                                                  <  1:137492/251‑1 (MQ=255)                                                                                                              ccACATGGAAGGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACCCGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAAtt                                                                                                                               <  2:69283/251‑1 (MQ=255)                                                                                                                               tggtggCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTggc                                                                                                              >  2:64872/1‑251 (MQ=255)                                                                                                                                      aaTCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGgcagca                                                                                                       <  1:64872/251‑1 (MQ=255)                                                                                                                                      aaTCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGgcagca                                                                                                       <  1:124115/251‑1 (MQ=255)                                                                                                                                          tGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTg                                                                                                   >  1:303217/1‑251 (MQ=255)                                                                                                                                                    gTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGAt                                                                                         >  2:48988/1‑251 (MQ=255)                                                                                                                                                                               gTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAgg                                                              <  2:162236/251‑1 (MQ=255)                                                                                                                                                                                 agagCTACCGGCAGGAAGTGAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTGATGAAGCTCCACCGGACGGACTGCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGTCGTCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCTAAGGGAGGcc                                                            <  1:47577/251‑1 (MQ=255)                                                                                                                                                                                       aCCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCatg                                                      <  2:85933/251‑3 (MQ=255)                                                                                                                                                                                           gCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCt                                                  <  1:180142/251‑1 (MQ=255)                                                                                                                                                                                             aGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTgg                                                >  1:440648/1‑251 (MQ=255)                                                                                                                                                                                                 aGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGTTGGTCCTCCGAGACGCTACCATTTGCGCGGATCCACACCTGACGATGGTGTTCTGCGATGGGGCCTTTCAGTGGGTTATGAAACGCCACCGGACGGACGTCCAGCGGACGATCGAAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGTTGCGCCAGCGATTGGGCGATTTGCGTTCCCGAAGGGAGGCCTTCAGGCGCTGGcgcg                                            <  2:151540/251‑1 (MQ=255)                                                                                                                                                                                                       gATCAGAAGCGTAACCGAGAAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATACACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATATTTCAGCACTGGAGGCAGCAGGAGCGCCAGCGATTGGGCGATTTGCGTTTCCGACGGGAGGCCATCAGGCGCTGGCGCGGAGGGc                                      >  2:17129/1‑251 (MQ=255)                                                                                                                                                                                                           aGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGCTCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTg                                  >  1:266490/1‑251 (MQ=255)                                                                                                                                                                                                                 gTAACCGAGAAGAGACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACGCCACCGGACGGACTTCGAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGtttttt                            <  1:51642/251‑1 (MQ=255)                                                                                                                                                                                                                    aCCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGAt                         >  1:117246/1‑251 (MQ=255)                                                                                                                                                                                                                     ccGAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGGTTTTTGATg                        <  2:214857/251‑1 (MQ=255)                                                                                                                                                                                                                       gAGCAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGtt                      <  2:100165/251‑1 (MQ=255)                                                                                                                                                                                                                          cAGATACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGa                   <  2:330647/251‑1 (MQ=255)                                                                                                                                                                                                                             aTACTGATGAACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAAc                <  2:25369/251‑1 (MQ=255)                                                                                                                                                                                                                                  gatgaACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAACCCGc            <  1:415970/250‑1 (MQ=255)                                                                                                                                                                                                                                  gatgaACGCGCAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAACCCGCt           <  1:233701/251‑1 (MQ=255)                                                                                                                                                                                                                                           gcAGGTCATCCGACACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAACCCGCTTCTGGTGcc  >  1:97869/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               CGCCGTCTTGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTGATGTTCGAAACCCGCTTCTGGTGCC  >  NC_000913/474351‑474835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑