Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	832,937	G→A	A5V (GCA→GTA)	ybiA ←	N‑glycosidase YbiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	832,937	0	G	A	97.4%	122.0 / NA	39	A5V (GCA→GTA)	ybiA	N‑glycosidase YbiA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (15/23);  minor base T (0/1);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTCCCCGTGCGCCGCAGCGGCAGATGATT  >  NC_000913/832695‑833174                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              gCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAAct                                                                                                                                                                                                                                       <  2:136251/251‑2 (MQ=255)                gATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCAt                                                                                                                                                                                                                        >  2:46117/1‑251 (MQ=255)                   cgcgCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTATTGCATGACGTGTTGGATCCTTTGTACTCAAACGGGCATTAAACCGCATTAt                                                                                                                                                                                                                     >  2:329470/1‑251 (MQ=255)                      gcCCCATGCGTGCGGCTACCATAGGGGAAGAATCCCTACGGATCTCTTCGCTGTATTGTTCATCAAGGAATTTTTTTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGCGCAGTAAACCGCATTATGtt                                                                                                                                                                                                                  <  2:51541/251‑1 (MQ=255)                               gtgcgGCGAACATAGGGGAAGAAACCCGACGTATCTCTTCGCGGTATTTTTCATCAAGGAATTTATGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCGTGATGGGCCAGGCGGCAAAGTTAGAAAAATCTCCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGACCCTGTGTACGCGAACGGCCATTAAACCGCATTATGTTGGTGGTTAt                                                                                                                                                                                                         <  2:281837/251‑1 (MQ=255)                                  cggcgACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAtt                                                                                                                                                                                                        <  2:71692/249‑1 (MQ=255)                                  cggcgACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAtt                                                                                                                                                                                                        >  1:71692/1‑249 (MQ=255)                                   ggcgACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCg                                                                                                                                                                                                     <  1:46117/251‑1 (MQ=255)                                      gACCATAGGGGAAGAAACCCGACGGATCTCGTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTGCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATGGCGAGc                                                                                                                                                                                                  <  2:266906/251‑1 (MQ=255)                                              gggAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTcc                                                                                                                                                                                          <  2:238049/251‑1 (MQ=255)                                                gaagaaACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcag                                                                                                                                                                                        <  2:119924/251‑1 (MQ=255)                                                         cGACGGATCTCTTCACGGTATTTTTCATCAATGAATGTTTGAGCCTGAAAATAGTGGTCTGAGGTAGGCCAAGTTTGCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTGATGATGGTGTCTTGCATGACGTGTTGGGTCCTTTGTACTCGAACGGGCATTAAAGCGCATCATTTTGGTGGTTAGCGCGAACCGCTTGCcagaagcataaa                                                                                                                                                                               <  2:204044/251‑8 (MQ=255)                                                            cGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGAGGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGTGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAAc                                                                                                                                                                            <  1:420960/251‑1 (MQ=255)                                                            cGGATCTCTTCGCGGTATATTTCATCAAGGAATTTTTGTACCTGAAAATAGTGTTATGAGGGAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCCAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGACACAGAAAAAc                                                                                                                                                                            >  1:245340/1‑251 (MQ=255)                                                                     tCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTAcc                                                                                                                                                                     <  1:347783/249‑1 (MQ=255)                                                                             tttttCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAAcc                                                                                                                                                           >  2:149374/1‑251 (MQ=255)                                                                                         gAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGc                                                                                                                                                                                                      >  1:278672/1‑196 (MQ=255)                                                                                         gAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATGATGTTGGTGGTTATTGc                                                                                                                                                                                                      <  2:278672/196‑1 (MQ=255)                                                                                             ttttGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAat                                                                                                                                            <  1:227517/250‑1 (MQ=255)                                                                                             ttttGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAat                                                                                                                                            <  1:134602/250‑1 (MQ=255)                                                                                                          aaTAGTGTTCTGAGGTAGGCCAGGTTGTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTtata                                                                                                                              <  1:42135/251‑1 (MQ=255)                                                                                                             aGTGTTCTGAGGAAGGCCACGTTTTCCGGTCAAGCTTCACGCGACAGGCGGCAAAGCTAGAAAACTCACCGTAGACATCGCTGGAGCGGCAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAAACATTACCCCTGAAAACCGACAAATGACACAATATTGGCTGGTTATAc                                                                                                                             >  2:90480/1‑249 (MQ=255)                                                                                                                       ggTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGTTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTACAGGt                                                                                                                 <  2:28710/251‑1 (MQ=255)                                                                                                                              cAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTtctc                                                                                                           >  2:79774/1‑250 (MQ=255)                                                                                                                                    ttaccgttaccctggATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAGATTTATGATGGTGTCTTTCGTGAAGTGTTGGATCCGTCGATCTCGAACGGGCATTAAACCGCATTATGTTGCTGGTTAGTGCGAGCCGCTTTCCATAAACATAAAAACCATGACCCCTGAAAACCGAAAAATTCCACAATATTGGCTGTTTATGCTGTATTCCAGGtttgcgcgtggcc                                                                                                     <  2:284178/236‑11 (MQ=255)                                                                                                                                        cGTCATCCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATGATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAAGATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAAc                                                                                                <  1:182130/251‑1 (MQ=255)                                                                                                                                                                cAAAGTTAGAAAAATCCCCGTAGTCATCTCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCTTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAACCCATTACCCCTGAAAACCGAAAAATGCCCCAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTgc                                                                        <  1:358007/251‑1 (MQ=255)                                                                                                                                                                                    tgcgcttcctatctGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGACCCTTTGTACTCGAACGTGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAACCCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCtt                                                     <  1:175882/237‑1 (MQ=255)                                                                                                                                                                                      gTCATCGCTGGTGCTGGAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGAGGTTATTGAGCGCCGCTTTCCAGAAAAAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTc                                                    >  2:372921/1‑249 (MQ=255)                                                                                                                                                                                               ggTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAACGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAAc                                              >  2:146311/1‑246 (MQ=255)                                                                                                                                                                                                  gCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATACTTTGTACTCGAACGGGCCTTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCtt                                                     >  2:175882/1‑236 (MQ=255)                                                                                                                                                                                                               atnagggTGTCTTGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCGGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTccc                          <  2:407697/245‑1 (MQ=255)                                                                                                                                                                                                               atgatgGTGTCTTGCAGGACGTTTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTcccc                         <  1:155774/251‑1 (MQ=255)                                                                                                                                                                                                                          ttGCATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTCCCCGTGcgccgc                >  2:392893/1‑249 (MQ=255)                                                                                                                                                                                                                             cATGACGTGTTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTCCCCGTGCGCCGCAGCgg           >  1:7262/1‑251 (MQ=255)                                                                                                                                                                                                                                   gtgtTGGATCCTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGTTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATAAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTCCCCGTGCGCCGCAGCGGCAgatg     <  2:364201/251‑1 (MQ=255)                                                                                                                                                                                                                                      ttGGATCCTTTGTACTCGAACGCGCATTAAACCGCATTCTGTTGCTGGTTATTGCGAGCCGCTTTCCAGAAACAGACAAACCATTACCCCTGAAAACCGAAACATGCCCCAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTCCCCGTGCGCCGCAGCGGCAGATGAtt  >  2:194678/1‑251 (MQ=255)                                                                                                                                                                                                                                             cTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCATGTTTTCTCATgg                                                                                                       <  2:262413/143‑1 (MQ=255)                                                                                                                                                                                                                                             cTTTGTACTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATgg                                                                                                       >  1:262413/1‑143 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              GCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGTTTTCTCATGGCATTAACCGCCGCGCTTAAAGCGCAAATTGCCGCCTGGTATAAGGCGCTTCAGGAACAGATCCCCGACTTTATTCCCCGTGCGCCGCAGCGGCAGATGATT  >  NC_000913/832695‑833174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑