Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	896,177	G→A	G15E (GGA→GAA)	potH →	putrescine ABC transporter membrane subunit PotH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	896,177	0	G	A	100.0%	182.7 / NA	51	G15E (GGA→GAA)	potH	putrescine ABC transporter membrane subunit PotH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/26);  total (25/26)

CGCGGTGGGGGAGGTGATACACATTGCCTATCTCGGCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGGATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCT  >  NC_000913/895935‑896414                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              cgcgGTGGGGGAGGTGATACACATTGCCTATCTCGGCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCACATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGACACCGCCGGGCGAATTTAAGc                                                                                                                                                                                                                                       >  1:47752/1‑251 (MQ=255)     gTGGGGGAGGTGATACACATTGCCTATCTCGGCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCtgtg                                                                                                                                                                                                                                   <  2:161514/251‑1 (MQ=255)        ggggAGGTGATACACATTGCCTATCTCGGCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGtt                                                                                                                                                                                                                                >  1:111978/1‑251 (MQ=255)          ggaggaGATACACATTGCCTATCTCGGCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGt                                                                                                                                                                                                                              >  1:311693/1‑251 (MQ=255)           gAGGTGATACACATTGCCTATCTCGGCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTc                                                                                                                                                                                                                             <  2:311693/251‑1 (MQ=255)                  tACACATTGCCTATCTCGGCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTg                                                                                                                                                                                                                      >  1:287778/1‑251 (MQ=255)                      caTTGCCTATCTCGGCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCCCCCAGCTACAAAACGCCCATCTTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCaaa                                                                                                                                                                                                                  <  2:403938/251‑1 (MQ=255)                                  cGGCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATggg                                                                                                                                                                                                       <  1:433346/250‑1 (MQ=255)                                    gCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGgcgc                                                                                                                                                                                                    >  1:126065/1‑251 (MQ=255)                                      gATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCa                                                                                                                                                                                                   >  2:91396/1‑250 (MQ=255)                                                      aCGTTCGTCTGAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTg                                                                                                                                                                                  <  1:410508/251‑1 (MQ=255)                                                          tCGTCTGAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCat                                                                                                                                                                              >  1:100283/1‑251 (MQ=255)                                                                gAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCt                                                                                                                                                                        <  1:67071/251‑1 (MQ=255)                                                                   aaGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGt                                                                                                                                                                     >  1:128463/1‑251 (MQ=255)                                                                    aGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGtt                                                                                                                                                                    >  1:447717/1‑251 (MQ=255)                                                                     gTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGTTTg                                                                                                                                                                   <  2:126385/251‑1 (MQ=255)                                                                       gggCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGa                                                                                                                                                                  <  1:319092/250‑1 (MQ=255)                                                                        ggCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATc                                                                                                                                                                >  1:341384/1‑251 (MQ=255)                                                                          cAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCtt                                                                                                                                                              <  1:115680/251‑1 (MQ=255)                                                                               gatTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGt                                                                                                                                                         <  1:178563/251‑1 (MQ=255)                                                                                   aGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTc                                                                                                                                                      <  2:126065/250‑1 (MQ=255)                                                                                              aCAAAACGCCCATTGTCACCGTAAAGGGTTAGCGACCTGGGGGGACGAAGTTGGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCGGACGGTTTAAGGAGCAAAGATAAGTACACTGGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCAtt                                                                                                                                            <  2:306291/249‑1 (MQ=255)                                                                                              aCAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTc                                                                                                                                          <  2:153986/251‑1 (MQ=255)                                                                                               cAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGTCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCt                                                                                                                                         <  1:417871/251‑1 (MQ=255)                                                                                                aaaaCGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCAtttatg                                                                                                                                        >  1:47504/1‑248 (MQ=255)                                                                                                 aaaCGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGa                                                                                                                                       <  1:247843/251‑1 (MQ=255)                                                                                                       ccATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCt                                                                                                                                 >  1:126807/1‑251 (MQ=255)                                                                                                           cgtcatcgtAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTaa                                                                                                                             <  2:147270/251‑1 (MQ=255)                                                                                                            gtcatcgtAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAATTCGACAGCTGTTTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCGGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTaaa                                                                                                                            <  2:189557/251‑1 (MQ=255)                                                                                                            gtcatcgtAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGCTTGTTTTTaa                                                                                                                             >  2:78940/1‑250 (MQ=255)                                                                                                                 ggtAAAGGGTTACCGACCTGGGGCGACGAAGTGCTTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGCGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAAGTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTCCCATTTCTGATTGTCTTTAAAATAAg                                                                                                                       <  1:172400/250‑1 (MQ=255)                                                                                                                 cgtAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGGTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTGCTGCTGCCATTTCTGATTGTCTTTAAAATaa                                                                                                                        <  1:166846/250‑1 (MQ=255)                                                                                                                     aaGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTg                                                                                                                   <  2:126807/251‑1 (MQ=255)                                                                                                                                aCCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGgc                                                                                                        >  2:230071/1‑251 (MQ=255)                                                                                                                                              aGTGCGTGTGTGCTGGGAAGTGGACAGCTGTGTGGTGCGGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCAc                                                                                          <  2:444341/251‑1 (MQ=255)                                                                                                                                                               aaGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATg                                                                         >  1:437552/1‑251 (MQ=255)                                                                                                                                                                   ggACAGATGTGTGGTGATGAAGGTTTAAGGAGCAAAGATGAGTAACCTTGCACCTGCTGCCCAGTCGAAACAGCCGGGAGAATTTACGCTGTGGTTGTAGCAGCTGCAAATGAAGCATGGGAGCAAACTGGTCATTGCGTTGCCCTATATCTGGTTGATCTTGCTGTTTATGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAgg                                                                     >  2:425947/1‑250 (MQ=255)                                                                                                                                                                    gACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTg                                                                    >  1:2712/1‑251 (MQ=255)                                                                                                                                                                           gtgtgGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGAc                                                             >  1:18931/1‑251 (MQ=255)                                                                                                                                                                           gtgtgGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGAc                                                             >  2:398256/1‑251 (MQ=255)                                                                                                                                                                                          tttAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATc                                              >  2:444424/1‑251 (MQ=255)                                                                                                                                                                                           ttAAGGAGCAAAGATGATTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATc                                              <  2:192842/250‑1 (MQ=255)                                                                                                                                                                                           ttAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTTGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGAGGGAGTGGGCTGACGGGCAACTGTCCATc                                              <  2:447717/250‑1 (MQ=255)                                                                                                                                                                                              aGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCActc                                          >  1:392396/1‑251 (MQ=255)                                                                                                                                                                                                           aGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTa                              >  1:337757/1‑250 (MQ=255)                                                                                                                                                                                                              acacTTGAACCTGCTGCCCAGTCGAAACCGCCTGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCTGTAAtt                           <  1:346059/250‑1 (MQ=255)                                                                                                                                                                                                                        ctgctgCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCCTTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTg                >  2:171130/1‑251 (MQ=255)                                                                                                                                                                                                                        ctgctgCCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTg                >  1:8740/1‑251 (MQ=255)                                                                                                                                                                                                                             gcCCAGTCGAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACcg            <  1:230071/250‑1 (MQ=255)                                                                                                                                                                                                                                 agtggAAACCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGAt       <  2:243589/247‑1 (MQ=255)                                                                                                                                                                                                                                      aaaCCGCCGGGCGAATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGct  >  2:195669/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              CGCGGTGGGGGAGGTGATACACATTGCCTATCTCGGCGATCTTTCGGTGTATCACGTTCGTCTGAAAAGTGGGCAGATGATTAGCGCCCAGCTACAAAACGCCCATCGTCATCGTAAAGGGTTACCGACCTGGGGCGACGAAGTGCGTTTGTGCTGGGAAGTGGACAGCTGTGTGGTGCTGACGGTTTAAGGAGCAAAGATGAGTACACTTGAACCTGCTGCCCAGTCGAAACCGCCGGGCGGATTTAAGCTGTGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTGGTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCT  >  NC_000913/895935‑896414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑