Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	969,254	G→A	E297K (GAA→AAA)	lpxK →	tetraacyldisaccharide 4'‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	969,254	0	G	A	100.0%	136.6 / NA	42	E297K (GAA→AAA)	lpxK	tetraacyldisaccharide 4'‑kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/13);  total (29/13)

GCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTGGAGGATATTCCGGCA  >  NC_000913/969008‑969496                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCaaaaga                                                                                                                                                                                                                                                 <  1:17494/251‑1 (MQ=255)         tttACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCAGCCGCCGCGCTTTTTTGCCACGCTGCAGATGTGTGGCGTACAAACGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATTCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATtggg                                                                                                                                                                                                                                         >  1:262148/1‑250 (MQ=255)          ttACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATTCCGGGCCTTTGCAAAAGAAAATtggtg                                                                                                                                                                                                                                        >  2:252293/1‑251 (MQ=255)            ccGTACCGGGACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATtggtgg                                                                                                                                                                                                                                       <  2:352077/249‑1 (MQ=255)               tACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTATTTGAACCATGCGGATGTCAGTGCGTTGGGAAGCGCCGGGGAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCATTGCAAAAGAAAATTGGTGGTAtt                                                                                                                                                                                                                                   >  1:309143/1‑251 (MQ=255)                aCCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTAttt                                                                                                                                                                                                                                  <  2:262173/251‑1 (MQ=255)                         cgTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTACCTGt                                                                                                                                                                                                                            >  1:176768/1‑248 (MQ=255)                            tgtgACGTTGCTCAGCTTGAGCATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGGCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACg                                                                                                                                                                                                                      <  1:37207/250‑1 (MQ=255)                            tgtgACGTTGCTCAGCTTGAACATGTAGAGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGACTGT‑AGaagc                                                                                                                                                                                                                     >  1:109455/1‑248 (MQ=255)                                                  aTGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGca                                                                                                                                                                                                                    >  2:364823/1‑230 (MQ=255)                                                  aTGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGca                                                                                                                                                                                                                    <  1:364823/230‑1 (MQ=255)                                                               ggCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTa                                                                                                                                                                                  <  1:2996/251‑1 (MQ=255)                                                                      aTTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGCCGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACt                                                                                                                                                                           >  2:343222/1‑251 (MQ=255)                                                                              tCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGGGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAACGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAATCTGCTTACGCAACTAACCNTGc                                                                                                                                                                   >  2:406616/1‑251 (MQ=255)                                                                                cgccgcGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGCAAACTGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTg                                                                                                                                                                 >  2:131351/1‑251 (MQ=255)                                                                                      gcTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACAGGAAAAATGTGTACCGCTGGCCGATAATCAGTCTTTGAACCAAGCGGATGTCAGAGCGATGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGTCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGAGAAACTGCTTACGCAACTAACCTTGCTGGCTTCt                                                                                                                                                           >  1:401795/1‑251 (MQ=255)                                                                                                cACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGt                                                                                                                                                 >  2:102197/1‑251 (MQ=255)                                                                                                    cTGAAGCTGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTAAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTAcg                                                                                                                                             >  1:40036/1‑251 (MQ=255)                                                                                                             tgtgGCGTACCATCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGAGAAATGCCGGGCCTTTGCAAAAGAAAATTGATGGTATTTGCGTGT‑AGACGCACAGCTTTCAGGTGATGAACACGCGATACTGCTCACGCAACTAAACTTGCTGGCTTCTGGCAACTAGTTACGCcgcgcc                                                                                                                                      >  2:81819/1‑247 (MQ=255)                                                                                                                   gTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCg                                                                                                                              <  1:35792/251‑1 (MQ=255)                                                                                                                        aCCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGCTGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTg                                                                                                                          >  2:309630/1‑250 (MQ=255)                                                                                                                         ccGGAAAAATGTGTTCCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGAt                                                                                                                        <  1:318222/251‑1 (MQ=255)                                                                                                                                  tgtgTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGCTGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATg                                                                                                               >  1:41236/1‑251 (MQ=255)                                                                                                                                              gCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGcc                                                                                                   <  1:210630/251‑1 (MQ=255)                                                                                                                                                       caGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCtctc                                                                                          <  2:26224/251‑1 (MQ=255)                                                                                                                                                                       cGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGt                                                                          >  1:81157/1‑251 (MQ=255)                                                                                                                                                                          aTGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTg                                                                                                                                                          <  2:246037/168‑1 (MQ=255)                                                                                                                                                                                          aaGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATACGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGcac                                                       >  1:136895/1‑251 (MQ=255)                                                                                                                                                                                            gcgcCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACAAGCGAAACTGCTTACGCAACTAACATTGCTGTCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCACGTAATCTTCACCTTGCCGcaca                                                      >  1:407817/1‑250 (MQ=255)                                                                                                                                                                                              gcCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAgg                                                   >  1:402929/1‑251 (MQ=255)                                                                                                                                                                                               ccGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGc                                                  >  1:442863/1‑251 (MQ=255)                                                                                                                                                                                               ccGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGc                                                  >  1:371201/1‑251 (MQ=255)                                                                                                                                                                                                       aCGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTaa                                           >  1:222303/1‑250 (MQ=255)                                                                                                                                                                                                           tGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGc                                                                                                                                   <  2:217001/158‑1 (MQ=255)                                                                                                                                                                                                           tGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGc                                                                                                                                   >  1:217001/1‑158 (MQ=255)                                                                                                                                                                                                                  gACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCccgcc                               >  1:30960/1‑251 (MQ=255)                                                                                                                                                                                                                  gACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCccgcc                               >  2:81565/1‑251 (MQ=255)                                                                                                                                                                                                                            gATGCGGTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGcgtc                     <  2:81157/251‑1 (MQ=255)                                                                                                                                                                                                                                 ggTGAAATGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTgg                >  1:423671/1‑251 (MQ=255)                                                                                                                                                                                                                                  gTGAAATGCCGGGCATTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTgg                >  1:368027/1‑250 (MQ=255)                                                                                                                                                                                                                                        tGCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCAGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTCGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGCGCGTCGTTGGAGGatat         >  2:85592/1‑251 (MQ=255)                                                                                                                                                                                                                                         gCCGGGCCTTTGCAAAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTGGAGGATAtt        >  1:198590/1‑251 (MQ=255)                                                                                                                                                                                                                                               ccTTTGCAAAAGAAAATGGGTGGTATTTGC‑TGTTAGACGCACAGCTTTCAGGTGATGACCCAGCGAAACTCCTTACGCATCTAACCTTGCTGACTTCTGGCATCTAGTTACGCCGCGCCAGAGTTCGATTGACGGAGCAATGAATGTCGCGTCCGCTCCTCTCCTTTGCTGTTCCGCGTAATCTTCCCCTTGCCGCACAAGGCCTTTTAAACAACCCCCGCCGTCGAGCGTCGTTGGAGGATATTCCGGCa  <  2:401795/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GCGGTGAATTTACGTACCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCATCCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCGCTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAAACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGT‑AGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTGGAGGATATTCCGGCA  >  NC_000913/969008‑969496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑