Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,105,328	G→A	intergenic (+35/‑86)	csgC → / → ymdA	curlin chaperone/uncharacterized protein YmdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,105,328	0	G	A	100.0%	119.7 / NA	34	intergenic (+35/‑86)	csgC/ymdA	curlin chaperone/uncharacterized protein YmdA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/15);  total (19/15)

CAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAGAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAA  >  NC_000913/1105084‑1105570                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   cAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGGCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAAt                                                                                                                                                                                                                                               <  1:116051/250‑1 (MQ=255)  aaaTATTGTCCCTGCGCGAAGGCAGTTCAAGGCAAAGTCATACGAAGCAAGAAAAGGCCCTTTCATTGCCTGCTAATCAACCCAGTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTAGTGTTTCTGATGGACAGTTACTTCATTTAGCACAACAATGGCCGCCCTCTGCAGAAAAGTCTTAATTGTTTGAAATATCGAGCATAAGATGAATCGGGAAAGAATgg                                                                                                                                                                                                                                             <  2:310418/251‑1 (MQ=255)      atTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTctg                                                                                                                                                                                                                                         >  2:354012/1‑251 (MQ=255)      atTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTGAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTctg                                                                                                                                                                                                                                         <  1:431470/251‑1 (MQ=255)                   gAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGcc                                                                                                                                                                                                                            <  2:2107/251‑1 (MQ=255)                       gCAGTTCAGGGCAAAGTCAGACGAAGCACGAAAAGACCCTTTCATTGCCTGCTAATCAACACATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTctg                                                                                                                                                                                                                                         >  2:225382/1‑234 (MQ=255)                       gCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTctg                                                                                                                                                                                                                                         <  1:225382/234‑1 (MQ=255)                                       tCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAac                                                                                                                                                                                                        >  1:81826/1‑251 (MQ=255)                                              aaGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAAccc                                                                                                                                                                                                 <  1:320014/251‑1 (MQ=255)                                                                      ccTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttcc                                                                                                                                                                           >  1:56050‑M1/1‑228 (MQ=255)                                                                             aTCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctg                                                                                                                                                                  >  2:209167‑M1/1‑221 (MQ=255)                                                                                  cccATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatc                                                                                                                                                             >  2:394148‑M1/1‑216 (MQ=255)                                                                                                gAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGACAATAGGTGTTCCTGTTTGTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTATTTTGTTGAAATATCGGGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattcc                                                                                                                                               >  1:423719‑M1/1‑202 (MQ=255)                                                                                                                   tCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagat                                                                                                                             >  1:80593‑M1/1‑183 (MQ=255)                                                                                                                       cGGACGATCGTGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaaccccttacaagcccgctgccgcagatattcc                                                                                                                        >  2:62408‑M1/1‑179 (MQ=255)                                                                                                                                 ggTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagc                                                                                                              <  2:56050‑M1/251‑83 (MQ=255)                                                                                                                                            ttgttACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagc                                                                                                   >  1:269925‑M1/1‑158 (MQ=255)                                                                                                                                             tgttACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagc                                                                                                   <  1:209167‑M1/250‑94 (MQ=255)                                                                                                                                                    gTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactat                                                                                            <  2:220457‑M1/250‑101 (MQ=255)                                                                                                                                                                  gtaaattaatttatcacAACAGTGGCCGCCCGCTTCAGAAGAGTCTTAATTTGTTGAAATATAGAGCATAAGATGAATCTGGAAAGAATGGTATGCTGCGAATCAGCCAACATGAAAGTATGGATAACACAAAACTtggatttgcacctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattccagtggcgagcgataagcaagcgcactatgcggaggccattcgt                                                                             >  2:159296‑M1/9‑136 (MQ=255)                                                                                                                                                                                cacaACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGAAGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatctcttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcagatgccattcgttataatgctcgaac                                                               <  1:389344‑M1/251‑130 (MQ=255)                                                                                                                                                                                      aaTGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgcctcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctct                                                         <  2:81826‑M1/251‑136 (MQ=255)                                                                                                                                                                                      aaTGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctct                                                         >  1:43966‑M1/1‑116 (MQ=255)                                                                                                                                                                                         ggCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgca                                                      <  2:212893‑M1/251‑139 (MQ=255)                                                                                                                                                                                                                tAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaaccc                               >  2:14084‑M1/1‑90 (MQ=255)                                                                                                                                                                                                                 aaTTTGTTGAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgtgataatgctcgaacgcctctgcaaggtgctttgcggccgttaaccc                               <  2:293097‑M1/250‑162 (MQ=255)                                                                                                                                                                                                                     tgttgAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtct                           <  2:43966‑M1/250‑166 (MQ=255)                                                                                                                                                                                                                       ttgAAATATCGAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtctggt                        >  1:175708‑M1/1‑83 (MQ=255)                                                                                                                                                                                                                                 gAGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgcggttaacccgtctggtttgggcatg               >  1:65584‑M1/1‑73 (MQ=255)                                                                                                                                                                                                                                  aGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtctggtttggg                   >  2:183721‑M1/1‑72 (MQ=255)                                                                                                                                                                                                                                  aGCATAAGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtctggtttggg                   <  1:183721‑M1/245‑174 (MQ=255)                                                                                                                                                                                                                                      tAAGATGAATCTGGAAAGCATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttcaagacatctgttatcacttaacccattacaagcccgctgccgcagatattaccgtggcgagcgataacccagcgcactatgcggatgccattcgatataatgctcgaacgcctctgcaaggttctttgctgccgttaccccgtctggattgggcatgatactg         >  2:439634‑M1/1‑68 (MQ=255)                                                                                                                                                                                                                                       aaGATGAATCTGGAAAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttgctgccgttaacccgtctggtttgggcatgatactg         >  2:123985‑M1/1‑67 (MQ=255)                                                                                                                                                                                                                                             aaTCTGGAAAGAATGGACTGCTGCGAATCAGCCAACCTGAAAGAATGGATAACAAAACCATtggatttgcccctatatttccagacatctgtaatcacttaacccattacaagcccactgccgcagatatttccgtggcgagcgataacccagcgcactatgaggatgccattagttataatgctcgaacgcctctgcaaggttattagctgccgttaacccgtctggattgggcatgatactgatgtagt  >  1:388612‑M1/1‑61 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   CAAATATTGTCCCTGCGCGAAGGCAGTTCAGGGCAAAGTCAGACGAAGCAAGAAAAGACCCTTTCATTGCCTGCTAATCAACCCATTGCTTTGACGAAGTTGAGTTTAAATATTTCCCCGGACGATCGGGTGAAAATAGTTGTTACTGTTTCTGATGGACAGTCACTTCATTTATCACAACAATGGCCGCCCTCTTCAGAAAAGTCTTAATTTGTTGAAATATCGAGCATAAGATGAATCTGGAGAGAATGGTCTGCTGCGAATCAGCCAACCTGAAAGTATGGATAACACAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAA  >  NC_000913/1105084‑1105570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑