Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,251,527	C→T	R230C (CGC→TGC)	nfo →	endonuclease IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,251,527	0	C	T	97.1%	106.4 / NA	34	R230C (CGC→TGC)	nfo	endonuclease IV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (17/16);  minor base G (0/1);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGG  >  NC_000913/2251291‑2251772                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      gCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTc                                                                                                                                                                                                                                         >  1:201969/1‑251 (MQ=255)  cAGTAACTTAGGGTTTAAATTCGAACATCGCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCg                                                                                                                                                                                                                                        <  2:182456/251‑1 (MQ=255)      aaCTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCTTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGa                                                                                                                                                                                                                                    <  1:301770/251‑1 (MQ=255)          gaGGGTTTAAATTCGAACAGCTCTCGGCGATTATCGACGGCGCGGAAGATAAATCCCGCCTCTGCGTCTGCATTGATGCCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTAGCTGCGCGTGATGCACCTTAACGATGCGAAAAGCGCCTATGGCAGCCGCGGTGACGACGATCATAGCCTCGGTGAAg                                                                                                                                                                                                                                  <  2:437078/248‑1 (MQ=255)                      tCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCat                                                                                                                                                                                                                    >  1:252241/1‑251 (MQ=255)                            aactcGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCg                                                                                                                                                                                                              <  1:150162/249‑1 (MQ=255)                              ctcGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGtt                                                                                                                                                                                                            >  1:112441/1‑251 (MQ=255)                                  cggcgATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGc                                                                                                                                                                                                        >  1:132134/1‑251 (MQ=255)                                                ggCGTTGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCGCCTTAACGATCCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTGCCGCTGGATCATGCAGga                                                                                                                                                                                          <  2:230396/251‑1 (MQ=255)                                                          aTAAATCCCGCGTCGGTGTCGGCATTGATACCTGCAATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCCCCTTAACGATGCGAAAAGCGCCTTTGGTAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTc                                                                                                                                                                                <  1:228437/251‑1 (MQ=255)                                                                       cGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTc                                                                                                                                                                                                                      <  1:311613/200‑1 (MQ=255)                                                                       cGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTc                                                                                                                                                                                                                      >  2:311613/1‑200 (MQ=255)                                                                           gTCTGCATTGATCCCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGAt                                                                                                                                                               <  2:102540/251‑1 (MQ=255)                                                                                              aTGCTTTCGCTGCCGTGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTAGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg                                                                                                                                            >  2:344321/1‑251 (MQ=255)                                                                                                         gCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTgg                                                                                                                                   <  2:252241/249‑1 (MQ=255)                                                                                                               tATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCaga                                                                                                                              >  1:320939/1‑248 (MQ=255)                                                                                                                                   cGAATGCGAGAAAACCTTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGAGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGATGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCt                                                                                                                   >  2:339517/1‑239 (MQ=255)                                                                                                                                   cGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCt                                                                                                                   <  1:339517/239‑1 (MQ=255)                                                                                                                                          gagaACACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACNAAc                                                                                                >  2:413841/1‑251 (MQ=255)                                                                                                                                           agaAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACt                                                                                               <  2:203408/251‑1 (MQ=255)                                                                                                                                                                        gTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATa                                                                    <  2:140786/249‑1 (MQ=255)                                                                                                                                                                              tttAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGa                                                             >  2:395044/1‑250 (MQ=255)                                                                                                                                                                                tAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCATCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGaaa                                                           <  1:245287/250‑1 (MQ=255)                                                                                                                                                                                   gTATCTGCGCGTGATGCACCTTAACGATGCGAAAAGCACCTGTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGGCGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTTGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGTAAAAGga                                                       <  2:222200/251‑1 (MQ=255)                                                                                                                                                                                    tATCTGCGCGGGATGCACCTTAACGATGCGCAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGCAAAgg                                                        >  1:372000/1‑249 (MQ=255)                                                                                                                                                                                                                 gAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATc                                                   <  2:13417/225‑1 (MQ=255)                                                                                                                                                                                                                 gAAAAGCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATc                                                   >  1:13417/1‑225 (MQ=255)                                                                                                                                                                                                                   aaaGCACCTTTGGCAGCCGCGGTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGa                       <  2:372000/251‑1 (MQ=255)                                                                                                                                                                                                                      gCACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGGCATTATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGCCGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGTCAGAAGAGCTCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGGAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGACGTAACCCGCTGATTc                    >  1:5729/1‑251 (MQ=255)                                                                                                                                                                                                                       cACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTca                   <  1:327991/251‑1 (MQ=255)                                                                                                                                                                                                                       cACCTTTGGCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGACTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTc                    >  2:218634/1‑250 (MQ=255)                                                                                                                                                                                                                               gCAGCCGCGTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGNACg           >  2:419387/1‑251 (MQ=255)                                                                                                                                                                                                                                    cgcgTTGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAAtt      >  1:430397/1‑251 (MQ=255)                                                                                                                                                                                                                                         tGACTGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCgg  >  1:356092/1‑250 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      GCAGTAACTTAGGGTTTAAATTCGAACATCTCGCGGCGATTATCGACGGCGTGGAAGATAAATCCCGCGTCGGCGTCTGCATTGATACCTGCCATGCTTTCGCTGCCGGGTATGATTTGCGTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTTTAAGTATCTGCGCGGGATGCACCTTAACGATGCGAAAAGCACCTTTGGCAGCCGCGTTGACCGCCATCATAGCCTCGGTGAAGGCAATATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGG  >  NC_000913/2251291‑2251772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑