Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,645,029	G→A	L52F (CTC→TTC)	prlC ←	oligopeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,645,029	0	G	A	100.0%	158.2 / NA	46	L52F (CTC→TTC)	prlC	oligopeptidase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/25);  total (21/25)

AGTGCGTTATCAACCGCTTTCTTCTGCGCCGTGTTCAGCGTGGCGTAATGATCGCCATCGCGCAGGTCGCGATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTC  >  NC_000913/3644782‑3645271                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    aGTGCGTTATCAACCGCTTTCTTCTGCGCCGTGTTCAGCGTGGCGTAATGATCGCCATCGCGCAGGTCGCGATACGCTTTATACAGCCCTTCATGTTGCCCTGCCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGTGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAAtt                                                                                                                                                                                                                                                  <  1:299876/251‑1 (MQ=255)     cGTTATCAACCGCTTTCTTCTGCGCCGTGTTCAGCGTGGCGTAATGATCGCCATCGCGCAGGTCGCGATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTcc                                                                                                                                                                                                                                              >  1:395950/1‑251 (MQ=255)        tATCAACCGCTTTCTTCTGCGCCGTGTTCAGCGTTGCGTAATGATCGCCATCGCGCAGGTCGCGATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCa                                                                                                                                                                                                                                            <  1:127307/250‑1 (MQ=255)               cGCTTTCTTCTGCGCCGTGTTCAGCGTGGCGTACTGATCGCCATCGCGCAGGTCGCGATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACg                                                                                                                                                                                                                                    >  1:375044/1‑251 (MQ=255)                    tcttctGCGCCGTGTTCAGCGTGGCGTAATGATCGCCATCGCGCAGGTCGCGATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGTGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGcc                                                                                                                                                                                                                               >  2:152762/1‑251 (MQ=255)                             ccGTGTTCAGCGTGGCGTAATGATCGCCATCGCGCAGGTCGCGATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGct                                                                                                                                                                                                                      <  2:157240/251‑1 (MQ=255)                               gtgtTCAGCGTGGCGTAATGATCGCCATCGCGCAGGTCGCGATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGctac                                                                                                                                                                                                                    <  2:340023/251‑1 (MQ=255)                                      gCGTGGCGTAATGATCGCCATCGCGCAGGTCGCGATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACgcgc                                                                                                                                                                                                             <  1:429296/251‑1 (MQ=255)                                                  gATCGCCATCGCGCAGGTCGCGATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTcg                                                                                                                                                                                                 >  2:215846/1‑251 (MQ=255)                                                         aTCGCGCAGGTCGCGATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTGCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGt                                                                                                                                                                                          <  1:74725/251‑1 (MQ=255)                                                                    cgcgATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGcc                                                                                                                                                                               <  1:160936/251‑1 (MQ=255)                                                                    cgcgATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGcc                                                                                                                                                                               <  1:302974/251‑1 (MQ=255)                                                                    cgcgATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGc                                                                                                                                                                                <  1:340265/250‑1 (MQ=255)                                                                                      gCCCTTCATGTTACCCTACCCAGGTGATGTATTCCGACAGCAGCGGCCGGGCTTGTTCGTAGGCTTCAAGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGCGGCTGACCGGGGAGAAGATACGCCCCAACACAACGTCCACTTCCGCCAGCGGCTGGCAGAACTTTTACCAGGTGTACGGTGCCCCTTGCGCTAATACGCGCTCCACGTTTTCGCGGCCGTCGCTCAATGCCTTAGTACCGGCTGGAACg                                                                                                                                                             >  2:49375/1‑251 (MQ=255)                                                                                            cATGTTGCCCTACCCAGGTGCTGTACTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATg                                                                                                                                                        <  2:209060/250‑1 (MQ=255)                                                                                             aTGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGATGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGACACGGCTGGAACGACATGtt                                                                                                                                                      >  2:291753/1‑251 (MQ=255)                                                                                               gTTGCCCTACCCAGTTTCTGTATTCCGACAGCAGCGGCAGGGGTTGTTCGTAGGCCTCACGCAGGTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACTCATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGTCATGTTcc                                                                                                                                                    <  2:212787/251‑1 (MQ=255)                                                                                               gTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCCGGTGGCTGACCGGGGAGAAGATACGCCTCAACCCATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTTCGGTTCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTc                                                                                                                                                     <  1:144512/250‑1 (MQ=255)                                                                                                    ccTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCggg                                                                                                                                                 >  1:56697/1‑249 (MQ=255)                                                                                                      tACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTAACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCTGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGgaga                                                                                                                                              >  1:84321/1‑250 (MQ=255)                                                                                                           aGGTGCTGTATTCCGACCGCCGCGGCAGTGTTTGTTCTTAGGCTTCACCCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACCCATCGTCCACTTCCTCCAGCGGCTGGCAGAAATTTTCCCAGGTTTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGCGAAttt                                                                                                                                          <  2:311642/249‑1 (MQ=255)                                                                                                                cTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAggaa                                                                                                                                    >  1:98423/1‑247 (MQ=255)                                                                                                                      tCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCTGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAg                                                                                                                              <  2:84321/250‑1 (MQ=255)                                                                                                                                gcGGCAGGGTTTGTTCGTCGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCaa                                                                                                                     >  1:128141/1‑249 (MQ=255)                                                                                                                                    cAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAAGGGCGGCAATTCAAAggg                                                                                                                 >  2:268063/1‑249 (MQ=255)                                                                                                                                              tCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACg                                                                                                     <  2:125602/250‑1 (MQ=255)                                                                                                                                                  aGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTc                                                                                                <  2:110865/251‑1 (MQ=255)                                                                                                                                                  aGGCTTCACGCAGTTCCGGGCTAGTTTTCACCGAGTTCAGGTGGCGGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTc                                                                                                <  1:84390/251‑1 (MQ=255)                                                                                                                                                        cACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCAAGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGGAAACGGGGGCAATTCAAAGGGAGTCCAG‑AACGGATTCGGCAtt                                                                                          >  2:242481/1‑251 (MQ=255)                                                                                                                                                           gCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTcgc                                                                                       >  1:38006/1‑251 (MQ=255)                                                                                                                                                            cAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTcgcg                                                                                      >  1:322341/1‑251 (MQ=255)                                                                                                                                                                  cGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACCTGTTCCGGGAGAATTTTAGAAAAAGGAGGGAATTCAAAGGGCGTC‑AGTAACGGATTCGTCATTCGCGCACTcc                                                                                >  2:7352/1‑251 (MQ=255)                                                                                                                                                                          ttttCACCGAGTTCAGGTGGCTGACCGGGGAGCAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCAGTCCTGGTTaa                                                                         <  1:410797/250‑1 (MQ=255)                                                                                                                                                                                            ggCTGACCGGGGAGCAGATGCGCCCCATCTCTTCGTCCCCTCCCGCCAGCGTCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCGCCACGTTTTCGCGGCAGTCGTTCAATGCCCTAGTCACGGCTGGAACCACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCCGTCCTGGTTAAAGAGGTTAAGGAAGCGCt                                                       <  2:149165/250‑1 (MQ=255)                                                                                                                                                                                                  ccGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAgg                                                >  1:254115/1‑251 (MQ=255)                                                                                                                                                                                                   cGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGgc                                               <  1:286854/251‑1 (MQ=255)                                                                                                                                                                                                                 gCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAAtgt                                 >  1:438447/1‑251 (MQ=255)                                                                                                                                                                                                                       acacATCGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAg                           <  2:254115/251‑1 (MQ=255)                                                                                                                                                                                                                             cGTCCACTTCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATggg                     >  1:427246/1‑251 (MQ=255)                                                                                                                                                                                                                                     tCCGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAg               <  1:215846/249‑1 (MQ=255)                                                                                                                                                                                                                                      ccGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAgtgt            <  2:38006/251‑1 (MQ=255)                                                                                                                                                                                                                                      ccGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAgtg             <  2:395950/250‑1 (MQ=255)                                                                                                                                                                                                                                       cGCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTAGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCCTGGGGTTAAgggt            >  1:13657/1‑247 (MQ=255)                                                                                                                                                                                                                                        gCCAGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTAGCGCAGTCCTGGGTAAAGGGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCGTGGGGTTAAgtgt            >  2:385394/1‑249 (MQ=255)                                                                                                                                                                                                                                           aGCGGCTGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGa       <  2:160956/251‑1 (MQ=255)                                                                                                                                                                                                                                                 tGGCAGAAATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTc  >  1:242456/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    AGTGCGTTATCAACCGCTTTCTTCTGCGCCGTGTTCAGCGTGGCGTAATGATCGCCATCGCGCAGGTCGCGATACGCTTTATACAGCCCTTCATGTTGCCCTACCCAGGTGCTGTATTCCGACAGCAGCGGCAGGGTTTGTTCGTAGGCTTCACGCAGTTCCGGGCTATTTTTCACCGAGTTCAGGTGGCTGACCGGGGAGAAGATACGCCCCAACACATCGTCCACTTCCGCCAGCGGCTGGCAGAGATTTTCCCAGGTGTACGGTGCCCCTTGCGCTACTACGCGCTCCACGTTTTCGCGGCAGTCGTTCAATGCCTTAGTCACGGCTGGAACGACATGTTCCGGGAGAATTTTAGAAAACGGAGGCAATTCAAAGGGAGTC‑AGTAACGGATTCGTCATTCGCGCAGTCCTGGTTAAAGAGGTTAAGGAAGCGCTCAACAGGCGCTTTACATAATGTGTGTAGCATGGGGTTAAGTGTAGTGAATTTC  >  NC_000913/3644782‑3645271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑