Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,404,682	C→T	intergenic (‑117/‑59)	abgA ← / → abgR	p‑aminobenzoyl‑glutamate hydrolase subunit A/putative LysR‑type DNA‑binding transcriptional regulator AbgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,404,682	0	C	T	100.0%	87.6 / NA	26	intergenic (‑117/‑59)	abgA/abgR	p‑aminobenzoyl‑glutamate hydrolase subunit A/putative LysR‑type DNA‑binding transcriptional regulator AbgR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/12);  total (14/12)

TCTTCCGCAACAAGGGTGGCAGTGCGGAATTCCACCCAGCCAGACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCGGGCTTTTGTTGAAGTGGCTCGTCAGGGCAGCATTCGCGGAGCGAGCCGAATGTTGAATATGTCGCAACCGGCACTGAGTAAATCTATTCAGGAGCTAGAAGAAGGGTTAGCGGCGCAACTCTTTTTTCGCCGTAGTAAAGGCGTGACGTTAACTG  >  NC_000913/1404441‑1404927                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      tcttcCGCAACAAGGGTGGCAGTGCGGAATTCCACCCAGCCAGACTCTGCATAGTTATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGCCCCTGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTCCCCTGACACAATCATTTGCATTaa                                                                                                                                                                                                                                               <  1:173059/250‑1 (MQ=255)      cGCAACAAGGGTGGCAGTGCGGAATTCCACCCAGCCAGACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGt                                                                                                                                                                                                                                            >  2:57575/1‑248 (MQ=255)            aaGGGTGGCAGTGCGGAATTCCACCCAGCCAGACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGcc                                                                                                                                                                                                                                     >  2:114978/1‑249 (MQ=255)             aGGGTGGCAGTGCGGAATTCCACCCAGCCAGACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGt                                                                                                                                                                                                                                  <  1:308173/251‑1 (MQ=255)             aGGGTGGCAGTGCGGAATTCCACCCAGATAGACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGCATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTACAAAGTGAGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGCTTTTTCACAGTTTTAAACGTTCATACATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGt                                                                                                                                                                                                                                  >  2:315325/1‑251 (MQ=255)                  ggCAGTGCGGAATTCCACCCAGCCAGACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGAAGCCAGTTTCtt                                                                                                                                                                                                                             <  2:313779/251‑1 (MQ=255)                          ggAATTCCACCCAGCCAGACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGa                                                                                                                                                                                                                     <  2:451803/251‑1 (MQ=255)                           gAATTCCACCCAGCCAGACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTGTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGat                                                                                                                                                                                                                    <  1:90972/251‑1 (MQ=255)                                                                        ccAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATtc                                                                                                                                                                       >  2:288176/1‑251 (MQ=255)                                                                           gTGCGATAATTTTGGGGAAAGCGAATTAACAAATTAATTCAAAGAATCCATAACCTTTCCCATCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATtcatc                                                                                                                                                                    >  1:144478/1‑251 (MQ=255)                                                                                aTAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAAtt                                                                                                                                                               >  2:209700/1‑251 (MQ=255)                                                                                 tAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTc                                                                                                                                                              >  2:14730/1‑251 (MQ=255)                                                                                   aTTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCgg                                                                                                                                                            >  1:16053/1‑251 (MQ=255)                                                                                                                  cAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCGGGCTTTTGTTGAAGTGGCTCGTCAGGgcagc                                                                                                                              <  1:64262/250‑1 (MQ=255)                                                                                                                                                          gacacGGTTCACGTAGCGATAGTTTTTACTGATCACTAACTGATTTTTCACAGTTTTAGCCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCGTGTCAGGTAAAAATTCATCAAATTCGGGCTTTTGTTGAATTTGCTCGTCAGTGCAGCATTCGCGGAGCGAGTGGAATGTTGAATATGTCGCAACCGGc                                                                                     <  2:431115/247‑1 (MQ=255)                                                                                                                                                                 ttCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATATATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGTGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCGGGCTTTTGTTGAAGTGGCTCGTCAGGGCAGCATTCGCGGAGCGAGCCGGCTGTTCAATTTGTGGCGAGCGGCACTGAgg                                                                              >  2:205616/1‑250 (MQ=255)                                                                                                                                                                   cACGTAGCGATAGTTGTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGACGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGTCTTCATGGCTTGTCATGTAAAAATTCATCAAATGCGGGCGTTTGTTGAAGTGGCTCTTTTGGGCAGCATTCGCGGAGCGGGCCGAATGTTGAATATGTCGCAACCGGCACTGAGTaa                                                                            <  1:411451/251‑1 (MQ=255)                                                                                                                                                                           gATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCGGGCTTTTGTTGAAGTGGCTCGTCAGGGCAGCATTCGCGGAGCGAGCCGAATGTTGAATATGTCGCAACCGGCACTGAGGAAATCTAttt                                                                    >  1:860/1‑250 (MQ=255)                                                                                                                                                                                    tACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCGGGCTTTTGTTGAAGTGGCTCGTCAGGGCAGCATTCGCGGAGCGAGCCGAATGTTGAATATGTCGCAACCGGCACTGAGTAAATCTATTCCGGCGCTa                                                            >  2:2487/1‑250 (MQ=255)                                                                                                                                                                                            aCTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACATTCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCGGGCTTttgt                                                                                                                                                    >  1:407810/1‑154 (MQ=255)                                                                                                                                                                                            aCTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCGTTTCAGGTAAAAATTCATCAAATTCGGGCTTttgt                                                                                                                                                    <  2:407810/154‑1 (MQ=255)                                                                                                                                                                                                        ttCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCGGGCTTTTGTTGAAGTGGCTCGTCAGGGCAGCATTCGCGGAGCGAGCCGAATGTTGAATATGTCGCAACCGGCACTGAGTAAATCTATTCAGGAGCTAGAAGAAGGGTTAGCGGCGCa                                        <  1:57575/250‑1 (MQ=255)                                                                                                                                                                                                                   aaCCGTTCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCGGGCTTTTGTTGAAGTGGCTCGTCAGGGCAGCATTCGCGGAGGGAGCCGAATGTTGAATATGTCGCAACCGGCACTGAGTAAATCTATTCAGGAGCTAGAAGAAGGGTTAGCGGCGCAACTCTTTTTTCg                            >  1:86213/1‑251 (MQ=255)                                                                                                                                                                                                                         tCATAAATTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCGGGCTTTTGTTGAAGTGGCTCGTCAGGGCAGCATTCGCGGGGCGAGCCGGATGTTTAATATGTCGCAACCGGCACTGAGTAAATCTATTCAGGAGCTAGAAGAAGGGGTAGCGGCGCACCTCTTTTTTCGCCgt                        >  2:350520/1‑249 (MQ=255)                                                                                                                                                                                                                             aaaTTACCCTGACACAATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTTTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCGGGCTTTTGTTGAAGTGGCTCGTCAGGGCAGCATTCGCGGAGCGAGCCGAATGTTGAATATGTCGCAACCGGCACTGAGTAAATCTATTCAGGAGCTAGAAGAAGGGTTAGCGGCGCAACTCTTTTTTCGCCGTAGTaa                   <  1:30533/250‑1 (MQ=255)                                                                                                                                                                                                                                           caATCATTTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCGGGCTTTTGTTGAAGTGGCTCGTCAGGGCAGCATTCGCGGAGCGAGCCGAATGTTGAATATGTCGCAACCGGCACTGAGTAAATCTATTCAGGAGCTAGAAGAAGGGTTAGCGGCGCAACTCTTTTTTCGCCGTAGTAAAGGCGTGACGTTAAc    <  1:2487/251‑1 (MQ=255)                                                                                                                                                                                                                                             atcattTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCGGGCTTTTGTTGAAGTGGCTCGTCAGGGCAGCATTCGCGGAGCGAGCCGAATGTTGAATATGTCGCAACCGGCACTGAGTAAATCTATTCAGGAGCTAGAAGAAGGGTTAGCGGCGCAACTCTTTTTTCGCCGTAGTAAAGGCGTGACGTTAACTg  <  2:16053/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      TCTTCCGCAACAAGGGTGGCAGTGCGGAATTCCACCCAGCCAGACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTCGGGCTTTTGTTGAAGTGGCTCGTCAGGGCAGCATTCGCGGAGCGAGCCGAATGTTGAATATGTCGCAACCGGCACTGAGTAAATCTATTCAGGAGCTAGAAGAAGGGTTAGCGGCGCAACTCTTTTTTCGCCGTAGTAAAGGCGTGACGTTAACTG  >  NC_000913/1404441‑1404927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑