Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,484	G→A	P974S (CCG→TCG)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,484	0	G	A	100.0%	151.6 / NA	46	P974S (CCG→TCG)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/18);  total (28/18)

TGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAG  >  NC_000913/481239‑481722                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               tGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAACAGCGGCATAACGCCGAGGATAAACGCCCGCGAGGTCATCAGGATCGAACGt                                                                                                                                                                                                                                            >  2:177552/1‑250 (MQ=255)      acacGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACg                                                                                                                                                                                                                                       <  2:331388/250‑1 (MQ=255)       cacGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGc                                                                                                                                                                                                                                      >  2:184348/1‑250 (MQ=255)       cacGTTGTATCAATGATGATCCACAGTATGGCTGTGCTCGTTACCTTCATTTTTGCGGCTAAAGCGGCGGCGAACCACCACACAGAATACAGGACCGAAGAAGCTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGATATGCGCGCCGGAACCCGCACAAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCAAGCGAGGTCATCAGGATCGAACGTAAACGc                                                                                                                                                                                                                                      >  2:187878/1‑250 (MQ=255)        acGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCAt                                                                                                                                                                                                                                    >  2:165110/1‑251 (MQ=255)                    tgatgaTCGACAGTAGGGCTGTGCTCGATATCTTCATTCTTGCGGATAAAGCGGCGGCGAACCACCACAAAGAATACAGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCCGCGGCATAACGCCGAGGATAAACGCCAGCGAGGACATCAGGATCGAACGTAAACGCATccgcaccgcaac                                                                                                                                                                                                                        >  2:250128/1‑249 (MQ=255)                      atgatCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCa                                                                                                                                                                                                                       >  2:148893/1‑250 (MQ=255)                            gACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCg                                                                                                                                                                                                                >  1:289565/1‑251 (MQ=255)                              cAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCt                                                                                                                                                                                                              >  2:86831/1‑251 (MQ=255)                                    ggCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATc                                                                                                                                                                                                        <  1:226370/251‑1 (MQ=255)                                             cGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCttt                                                                                                                                                                                                >  1:272293/1‑250 (MQ=255)                                                tatCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACct                                                                                                                                                                                            >  1:221303/1‑251 (MQ=255)                                                 atCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACctt                                                                                                                                                                                           >  1:64672/1‑251 (MQ=255)                                                     tCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACcttctt                                                                                                                                                                                        <  1:92186/250‑1 (MQ=255)                                                         acTTGCGGCTAAAGCGGCGGGGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGAGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACATTTACCTTCTTTATcc                                                                                                                                                                                   >  2:66696/2‑251 (MQ=255)                                                          cTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTACGGTCACCATCCCGCCCATTACACCGGTACCTACCGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATccc                                                                                                                                                                                  >  1:60311/1‑250 (MQ=255)                                                               ggCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAg                                                                                                                                                                             <  1:376351/251‑1 (MQ=255)                                                                gCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCCTCAAGt                                                                                                                                                                            >  2:386863/1‑251 (MQ=255)                                                                                 ccaccaCAAAGAATACCGGAACGAAGAAGATTTCCAGTACCGTTGCGGTCACCAGCCCGCCCATTACACCGGTACCTACTGCGTTCTGCTCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTcgacg                                                                                                                                                           <  1:250128/251‑1 (MQ=255)                                                                                   accacAAAGAATACCGGAACGTGGAAGATTGCCAGAACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTATTGCGTTGTGCGCGCCGAAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGGAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGAACTTTACCTTCATTCTCCATCAAGTCTTTGGCGAATTcgacga                                                                                                                                                          >  2:244724/1‑250 (MQ=255)                                                                                          aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATc                                                                                                                                                  <  1:148893/251‑1 (MQ=255)                                                                                               aCCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGt                                                                                                                                              <  1:395583/250‑1 (MQ=255)                                                                                                     aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGc                                                                                                                                       >  1:286716/1‑251 (MQ=255)                                                                                                          gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGAc                                                                                                                                   >  1:106842/1‑250 (MQ=255)                                                                                                                    aGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGt                                                                                                                        >  1:335511/1‑251 (MQ=255)                                                                                                                     gTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGtt                                                                                                                       <  1:4526/251‑1 (MQ=255)                                                                                                                                   aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTAc                                                                                                         <  1:409674/251‑1 (MQ=255)                                                                                                                                   aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTAc                                                                                                         >  1:349039/1‑251 (MQ=255)                                                                                                                                       tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTgg                                                                                                     >  1:444296/1‑251 (MQ=255)                                                                                                                                         ccGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGaa                                                                                                   <  1:343321/251‑1 (MQ=255)                                                                                                                                                            cTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCatg                                                                                >  2:330170/1‑249 (MQ=255)                                                                                                                                                                 gCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCAc                                                                            <  1:184348/250‑1 (MQ=255)                                                                                                                                                                              cGGAACCAGCACCAGAACTGATAACCAGCGGCCTAACGCCAAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGcagcc                                                              >  1:383441/1‑251 (MQ=255)                                                                                                                                                                                 aaCCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGcagccagc                                                           >  1:405261/1‑251 (MQ=255)                                                                                                                                                                                 aaCCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGcagccagc                                                           <  1:19036/251‑1 (MQ=255)                                                                                                                                                                                        aCCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCAc                                                    <  2:403542/251‑1 (MQ=255)                                                                                                                                                                                                               tAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGAcc                             >  1:18700/1‑251 (MQ=255)                                                                                                                                                                                                                     cGAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATa                       <  1:86831/251‑1 (MQ=255)                                                                                                                                                                                                                      gAGGATAAACGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATa                       >  2:218956/1‑250 (MQ=255)                                                                                                                                                                                                                             aaCGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGgaga                >  2:114331/1‑250 (MQ=255)                                                                                                                                                                                                                              ccGCCAGCGAGGTCATCAGGATCGAACGGAAACGCATCCGCACCGCATCAAGCGTCGCTTCAGTCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAAGGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACACCCAGCGGAACGACCAGCATAACGGAGAAc              <  2:202002/250‑1 (MQ=255)                                                                                                                                                                                                                              aCGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAAc              <  2:106842/251‑1 (MQ=255)                                                                                                                                                                                                                              aCGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAAc              >  1:107482/1‑251 (MQ=255)                                                                                                                                                                                                                               cGCCAGCGAGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACg             >  2:274913/1‑251 (MQ=255)                                                                                                                                                                                                                                       aGGTCATCAGGATCGAACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGAc     <  1:295084/251‑1 (MQ=255)                                                                                                                                                                                                                                           catcaGGATCGAACGTAAACGCAGCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAg  <  1:404697/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               TGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAACCCAATGGTTGTGAGCAGGCCTACCTGGAAGTAAACGTCATTGGTCAGGCCACGGAAGGTGGCAGCCAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAG  >  NC_000913/481239‑481722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑