Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,623,523 G→A Q199* (CAG→TAG)  lplA ← lipoate‑‑protein ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,623,5230GA96.5% 186.1 / NA 59Q199* (CAG→TAG) lplAlipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (31/25);  minor base T (0/2);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCACCAGATCGTTACGTCCGGACGC  >  NC_000913/4623276‑4623766
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
gccagcgcgcGGGTTATACTGCCTTTTTCAACTCCGAAATGCAGTTCCGCGCCGCCCCAGGTAAATCTTTCATCCAGCAGAGGCGAGAATGCCGGGGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGGTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATTATTTCCGCCTCCACGCGCTCGCCATCATGGGCGAAAAAGTCCTCGGTTATGGCCTCGCAAACCTACTc                                                                                                                                                                                                                                                  <  2:275536/245‑1 (MQ=255)
    gggCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATgg                                                                                                                                                                                                                                              >  1:40097/1‑251 (MQ=255)
               atatGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCgg                                                                                                                                                                                                                                   >  1:148075/1‑251 (MQ=255)
                  tGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGCTGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGCAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGcc                                                                                                                                                                                                                                 >  2:435844/1‑249 (MQ=255)
                     ccTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCAAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAAc                                                                                                                                                                                                                               >  2:154801/1‑249 (MQ=255)
                           tCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCgg                                                                                                                                                                                                                        <  1:350677/250‑1 (MQ=255)
                            cAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTg                                                                                                                                                                                                                      <  1:306034/251‑1 (MQ=255)
                                       tGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGGGAGGTTGGTCAc                                                                                                                                                                                                           >  1:75952/1‑251 (MQ=255)
                                                aCGCCGACCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTAGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACg                                                                                                                                                                                                  >  1:355051/1‑251 (MQ=255)
                                                   ccgccCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTAc                                                                                                                                                                                               <  1:195560/251‑1 (MQ=255)
                                                    cgccCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCCCGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACGCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTAcc                                                                                                                                                                                              <  1:228129/251‑1 (MQ=255)
                                                     gccCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCg                                                                                                                                                                                             >  1:122086/1‑251 (MQ=255)
                                                       cccATGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGAc                                                                                                                                                                                           <  2:145653/251‑1 (MQ=255)
                                                           ggTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTaa                                                                                                                                                                                       <  1:341281/251‑1 (MQ=255)
                                                                aGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGGTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCt                                                                                                                                                                                  <  2:160354/251‑1 (MQ=255)
                                                                  cGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCttt                                                                                                                                                                                <  1:323901/251‑1 (MQ=255)
                                                                       aTCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTcgccg                                                                                                                                                                           >  1:326367/1‑251 (MQ=255)
                                                                                     agaATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGGTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCttttt                                                                                                                                                              >  2:278758/1‑250 (MQ=255)
                                                                                                   tGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGAt                                                                                                                                                >  1:255063/1‑250 (MQ=255)
                                                                                                                 ttCCCAGCTACTCTGGCGGGCCAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCAATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCCCGGTGAGGGTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGGTAGTTTGCCAggcggc                                                                                                                                 >  2:134880/1‑251 (MQ=255)
                                                                                                                    ccAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTGTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAGCCCACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTGTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGa                                                                                                                              <  1:380820/251‑1 (MQ=255)
                                                                                                                          aCTCTGTCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGATTTTTGTTCGGGAGGATGATTTCCGCTTCCACCCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTc                                                                                                                          <  2:168505/249‑1 (MQ=255)
                                                                                                                          aCTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTc                                                                                                                          <  1:428652/249‑1 (MQ=255)
                                                                                                                          aCTCTGGCGGGCAAAGGTTTCGGCGAAGTTGGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTc                                                                                                                          <  2:75952/249‑1 (MQ=255)
                                                                                                                               ggCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTTTTCGGGGAGATGATTTCCGCTTCCACGCCCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCa                                                                                                                      <  2:175101/248‑1 (MQ=255)
                                                                                                                                 cgggcaaattgtgtcacGAAGTTTGGAAAGTCGTGCTTTTTGTTCGGGGAGATGATTTCCGGTTCCCCGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCTTAAACCTCCTCATGGTTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCGCGCGGGAACGTACCGACGTAATGCCTTTCGTCGCCAGTTTCTTTTTAGCCTGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGat                                                                                                                 <  2:195469/235‑2 (MQ=255)
                                                                                                                                      aaaGGTTTCGGCGAAGTTTGGCAAGTCTGGCGGTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGc                                                                                                             >  1:62212/1‑250 (MQ=255)
                                                                                                                                         ggTTTCGGCGAATTTGGGCATGTCTGGCGTTTTGTTCGGGGAGATGATTTCATCTTCCCGGCGCTCGCCACAATGGGAGAAAAAGGGCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACGGCTTGGGGAGGTTGGCCACGCGGGAACGTACCGGCGTAAGGCCTTGCGCCTCCAGTTTCTTTTTAGCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAg                                                                                                          <  2:352361/250‑1 (MQ=255)
                                                                                                                                              cGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCg                                                                                                    <  2:345374/251‑1 (MQ=255)
                                                                                                                                                gcgcaGTTTGGCAAGGCTGGAATTTTGTTGGTGGAGATGAGTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCAGTGATGGCCTCGCAAACGTACTCGGTGGTGATCCCCGGCAACAACTCGGTAAGGTGGGTCTTGCGGGAACGTACCGACGGAATGCCTTGCGCGGCCAGTTTCTTTGTTTCCGGGTTGAGGTAGTTTGCCAGGCGGCTGAGGTCTTCATTGAGTAGCAAGGTGCCgtg                                                                                                  <  2:177003/247‑1 (MQ=255)
                                                                                                                                                gCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCgtg                                                                                                  >  2:343274/1‑251 (MQ=255)
                                                                                                                                                       ttGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGa                                                                                             >  1:8677/1‑249 (MQ=255)
                                                                                                                                                       ttGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGa                                                                                             >  2:22074/1‑249 (MQ=255)
                                                                                                                                                          gCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCcg                                                                                        >  2:58063/1‑251 (MQ=255)
                                                                                                                                                            aagcttggggTTTTGTTGGGGGAGATGATTTCCGCTGCCTCGCGCTCGCCATAATGGGCGAATAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGTCAACGGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTGTTTAGCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGACGCcgcg                                                                                      <  2:7387/242‑1 (MQ=255)
                                                                                                                                                             aGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCcgcg                                                                                      >  1:103256/1‑250 (MQ=255)
                                                                                                                                                                cTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCt                                                                                  >  1:218559/1‑251 (MQ=255)
                                                                                                                                                                                 gagaTGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGACGTGGTGGAAGCCGCGATCTTTg                                                                               >  2:116520/1‑237 (MQ=255)
                                                                                                                                                                                 gagaTGATTTCCGCTGCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGTAAGCCGCGATCTTTg                                                                               <  1:116520/237‑1 (MQ=255)
                                                                                                                                                                                  agaTGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGcc                                                                >  1:25347/1‑251 (MQ=255)
                                                                                                                                                                                  agaTGATTTCCGCTACCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGcc                                                                <  2:309365/251‑1 (MQ=255)
                                                                                                                                                                                    atgatTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCg                                                               >  1:275806/1‑250 (MQ=255)
                                                                                                                                                                                                 ccACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTg                                                 >  1:166361/1‑251 (MQ=255)
                                                                                                                                                                                                  cACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGc                                                >  1:70737/1‑251 (MQ=255)
                                                                                                                                                                                                    cgcgCTCGCCATAATGTGCGAAAAAGGCCTCGGTTATGTCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCgg                                              <  2:355051/251‑1 (MQ=255)
                                                                                                                                                                                                      cgcTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGGTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTc                                            >  2:233546/1‑251 (MQ=255)
                                                                                                                                                                                                             cATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCg                                     >  1:27127/1‑251 (MQ=255)
                                                                                                                                                                                                                aaTGGGCGAAAAAGGCCGCGGTTATGGCCTCGCAAGCCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTGCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCGTCGACg                                  <  1:134880/251‑1 (MQ=255)
                                                                                                                                                                                                                 aTGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACgg                                 >  2:425760/1‑251 (MQ=255)
                                                                                                                                                                                                                 aTGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGGACCGACTTAATGCCTTTCGCCGTCAGCTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCAGTGAGTAGCAAGGTGCCGTCGTGGAAGCCACGCTCTTGTGTTTCGCGTTAGGCAGAGCCTGAGACTTTGCGGTCGCCTTCGACgg                                 >  2:373259/1‑251 (MQ=255)
                                                                                                                                                                                                                  tGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGt                                >  1:132533/1‑251 (MQ=255)
                                                                                                                                                                                                                   gggCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGtt                               <  2:122086/251‑1 (MQ=255)
                                                                                                                                                                                                                           aaGGCCTCGGTTATGTCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGTAACGTACCTACTTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTTCCAGGCGGCTGATGTCGGCATTGAGTAGCAAGGTGCCTTGGTGGAAGCCTCGATCTTTGCCTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTcaccac                       <  1:279923/251‑1 (MQ=255)
                                                                                                                                                                                                                               ccgcGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCACCAGa                   <  2:25347/248‑1 (MQ=255)
                                                                                                                                                                                                                               ccTCGGTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCCCGCGGGCACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGCAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCaccacc                      >  2:17965/1‑248 (MQ=255)
                                                                                                                                                                                                                                    gTTATGGCCTCGCAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCACCAGATCGtt              >  2:374527/1‑251 (MQ=255)
                                                                                                                                                                                                                                       aTGGCCTCGCAAACCTTCTCAGGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCTCCAGATCGTTACg           <  2:62212/251‑1 (MQ=255)
                                                                                                                                                                                                                                          ggcTCGCAATCCTTCTCATGAGTAATCCCCGGTGGCAACTCGGTGAGGTTGGTCACGCTGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGATGTCTGCATTGAGTAACAAGGTTCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCCAGCCTTAAGCTATACGGTCGCCTTCGACGGTTTTCACCACCAGATCGTTACGTcc        <  2:49332/249‑1 (MQ=255)
                                                                                                                                                                                                                                                cAAACCTACTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCACCAGATCGTTACGTCCGGACGc  >  2:268418/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
ACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCACCAGATCGTTACGTCCGGACGC  >  NC_000913/4623276‑4623766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑