Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	705,414	G→A	A491T (GCT→ACT)	nagE →	N‑acetylglucosamine‑specific PTS enzyme IIABC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	705,414	0	G	A	100.0%	204.7 / NA	58	A491T (GCT→ACT)	nagE	N‑acetylglucosamine‑specific PTS enzyme IIABC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/34);  total (24/34)

CACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCAGCCGCAGGGACAATCGTGAAAATCTT‑CAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTAT  >  NC_000913/705182‑705658                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       cACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGtat                                                                                                                                                                                                                                      <  1:285230/251‑1 (MQ=255)       tCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATcg                                                                                                                                                                                                                                >  2:148835/1‑251 (MQ=255)          gcgcCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATcg                                                                                                                                                                                                                                <  2:117820/248‑1 (MQ=255)                tACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTgg                                                                                                                                                                                                                       >  1:266868/1‑251 (MQ=255)                  cAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTa                                                                                                                                                                                                                     <  1:188001/251‑1 (MQ=255)                      ggCTGACTCTGCCCGCGTTAACGATACGATGCGAAAACGTCTGTGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTAGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCCGACTGTACCAACCGCGGCATCTATCGCGGAGCTGGTATCGc                                                                                                                                                                                                                 >  1:382053/1‑251 (MQ=255)                           aCTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGAtt                                                                                                                                                                                                            <  1:437198/251‑1 (MQ=255)                                  ccGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTg                                                                                                                                                                                                     >  2:216731/1‑251 (MQ=255)                                   cgcgTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTg                                                                                                                                                                                                     >  1:191787/1‑250 (MQ=255)                                        tAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTc                                                                                                                                                                                                <  2:112408/250‑1 (MQ=255)                                             atacgatGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCa                                                                                                                                                                                          <  1:258583/251‑1 (MQ=255)                                               acgatGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACt                                                                                                                                                                                        >  1:355951/1‑251 (MQ=255)                                                cgatGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTg                                                                                                                                                                                       <  2:336456/251‑1 (MQ=255)                                                    gtgtAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATc                                                                                                                                                                                   >  2:444311/1‑251 (MQ=255)                                                                gggTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGGGATGTCGTGGCACTGGATCAGGTTCCTGACg                                                                                                                                                                       >  1:103438/1‑251 (MQ=255)                                                                       cctgggggcgggactcTGAACAAACAGACTGTTCAGGTGATTGTGGGCGCGAACGCAGCATCCATCGGCGATGCGATGAAGAAAGTCGTTCCCCGTGGTCCCGTCTCCGACGCGTCAGCTGAAACAACTCCGGCAACTGCCGAGCCTGAAGCAAAACCGCAGACTGTACCAAACGCCGTATCTATCGCGGAGCCGGTATCGCAGATTACCGGTGATGTCGTGCCACTGGATCAGGTTCCTGACGAAGCAtt                                                                                                                                                                >  2:136770/16‑251 (MQ=255)                                                                           gggTAGTGAAACGTAAATAACAGAGTATTGAGGTGATGGTTGGCGCGAAAGCAGAATCCATCGGCCATGGGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGTTGCGTCAGCTGAAACATCTCCGGCATCTGCCGCGCGTGTAGCAAAACCGCAGACGGTACCAGACGCGGTATCTATCGCTGAGCTCGTTTCGCCGATTACTGGTCATGTCGTTGCACTGGATCATGCTCGTGACGAACCATTCGcc                                                                                                                                                            <  2:224820/251‑1 (MQ=255)                                                                             gTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAGGCAGAAGCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAg                                                                                                                                                          <  2:376847/251‑1 (MQ=255)                                                                                        aaacaaacaGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTATCCGCTGCGTCAGCTGAAACAACTCCGGCATCTGCCGCGCCTGTAGCAAATCCGCATACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTGCCGGTGATGTCGTGGCACTTGATCAGGTTCCTGACGAAGCATGCGCCAGCAAAGCggtt                                                                                                                                                <  2:223941/249‑2 (MQ=255)                                                                                            aaacaGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCggtgggtg                                                                                                                                            >  2:206236/1‑250 (MQ=255)                                                                                              acaGACTATTCAGGTGATTGTTGGCTCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTAGCTATCGCGGAGCTGGTATTGCCGATTACCGGTGATGTCGTGGCACTGGTTCAGGTTCCTGACGAAGCATTCGCCAGGAAAGCGGTGGGTGACg                                                                                                                                         <  1:363064/251‑1 (MQ=255)                                                                                              acaGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACg                                                                                                                                         >  2:18674/1‑251 (MQ=255)                                                                                               caGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACgg                                                                                                                                        >  1:235789/1‑251 (MQ=255)                                                                                                aGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACggg                                                                                                                                       >  2:214119/1‑250 (MQ=255)                                                                                                       tCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCg                                                                                                                                 <  1:18835/250‑1 (MQ=255)                                                                                                         aGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTg                                                                                                                              <  2:191787/251‑1 (MQ=255)                                                                                                            tGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCAATGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGGGACGGTGTGGCGGtcaa                                                                                                                            >  2:116464/1‑247 (MQ=255)                                                                                                                    ggCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCggggcaaaccacaa                                                                                                                    >  1:263679/1‑238 (MQ=255)                                                                                                                    ggCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAg                                                                                                                    <  2:145401/250‑1 (MQ=255)                                                                                                                      cgcgAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATa                                                                                                                 <  1:113360/251‑1 (MQ=255)                                                                                                                        cgAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATaaa                                                                                                               <  1:108641/251‑1 (MQ=255)                                                                                                                         gAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGAAACCGACACATaaa                                                                                                               >  1:156139/1‑250 (MQ=255)                                                                                                                           aaGCAGAATCCATCGGCGATGCTATGAAGAAAGTCGTTTCCCGTGGTCCGGTCGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCTCGCCTGTAGCAAAACCGCAGACTGTACCAATCGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAAtc                                                                                                            <  1:279907/251‑1 (MQ=255)                                                                                                                             gCAGAATCCAGCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGGCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAAtcgt                                                                                                          <  2:263679/251‑1 (MQ=255)                                                                                                                              cAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAAtcgtc                                                                                                         <  1:148835/251‑1 (MQ=255)                                                                                                                                      aTCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGGAAACGGCAGATAAAATCGGCGTA‑TAAcc                                                                                                >  1:419588/1‑251 (MQ=255)                                                                                                                                             atgcgatgAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCAGCCGCa                                                                                         <  2:156139/251‑1 (MQ=255)                                                                                                                                              tgcgatgAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGGA‑Tcccccaccgc                                                                                          >  1:204914/1‑240 (MQ=255)                                                                                                                                              ggcgatgAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCAGCCGCAg                                                                                        <  2:235789/250‑1 (MQ=255)                                                                                                                                                cgatgAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCAGCCGCAggg                                                                                      <  1:135173/251‑1 (MQ=255)                                                                                                                                                         aaGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGGAACCGGCAAGTAAAATTGTCGTAATC‑CCAACCGCAGGGACaaacggg                                                                             >  1:162208/1‑246 (MQ=255)                                                                                                                                                           gTCGTTGCCCTTGGTCCGGTGGCCGTTGCGTCAGCTGAAACAACTCCGGCATCTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGTATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCTACAGATAAAATCGTCGTA‑TCACCAGCCGCAGGGACAATCGTGaa                                                                           <  1:446923/251‑1 (MQ=255)                                                                                                                                                                  cccGTGGTCCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCg                                                                                                                                 <  2:339056/191‑1 (MQ=255)                                                                                                                                                                  cccGTGGACCGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCg                                                                                                                                 >  1:339056/1‑191 (MQ=255)                                                                                                                                                                          ccGGTAGCCGCTGCGTCAGCTGAACCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAAACGACAGATAAAATCGTCGTA‑TCACCAGCCGCAGGGACAATCGTGAAAATCTT‑CAACacca                                                            <  2:103438/250‑1 (MQ=255)                                                                                                                                                                           cGGTAGCCGCTGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCAGCCGCAGGGACAATCGTGAAAATCTT‑CAACaccaac                                                          <  2:383796/251‑1 (MQ=255)                                                                                                                                                                              caGCCGCTGTGTCAGCTGAAACAACACCGGCAACGGCCGCGCTTGTAGCAAAGCCGCAGACTGTTCCAAGCGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGGGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCAGCCGCAGGGACAATCGTGAAAATCTT‑CAGCACCAACCAc                                                       <  2:266513/250‑1 (MQ=255)                                                                                                                                                                                gCCGCTGCGACAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCAGCCGCAGGGACAATCGTGAAAATCTT‑CAACACCAACCAcgc                                                     <  2:39533/251‑1 (MQ=255)                                                                                                                                                                                     tGCGTCAGCTGAAACAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCCGCCGCAGGGGCAAACGTGAAAATCTTACAA‑ACCAACCAcccggtct                                                >  1:355874/1‑244 (MQ=255)                                                                                                                                                                                               gatacAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCCGCCGCAGGGACAATCGTGAAAATCTT‑CAACACCAACCACGCGTTCTGCCTGGAAAc                                      <  1:307143/247‑1 (MQ=255)                                                                                                                                                                                                aaacAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCAGCCGCAGGGACAATCGTGAAAATCTT‑CAACACCAACCACGCGTTCTGCCTGGAAAcc                                     <  1:71654/248‑1 (MQ=255)                                                                                                                                                                                                              aCTGCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAGACCGAAAGATAAAATCGTCGTA‑TCACCCGCCGCCGGGGCAAACGGTAAAAACAT‑AAACACCCACCCCGCCGTCTGACCGGAAACCGAAAACAGCGCggg                       >  2:335674/1‑250 (MQ=255)                                                                                                                                                                                                                 gCCGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCAGCCGCAGGGACAATCGTGAAAATCTT‑CAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGAt                    <  2:266868/251‑1 (MQ=255)                                                                                                                                                                                                                  ccGCGCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCAGCCGCAGGGACAATCGTGAAAATCTT‑CAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGAtc                   <  1:82216/251‑1 (MQ=255)                                                                                                                                                                                                                   cgcgCCTGTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCAGCCGCAGGGACAATCGTGAAAATCTT‑CAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGAtcg                  <  1:214119/251‑1 (MQ=255)                                                                                                                                                                                                                       ccTNTAGCAAAACCGCAGACGGTACTAAACGCGGTATCTATCGCGGAGCTGGTAGCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTTGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCAGCCGCAGGGACAATCGTGAAAATCTT‑CAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATcgtcgt              <  2:421972/251‑1 (MQ=255)                                                                                                                                                                                                                          gTAGCAAAACCGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGGAAAAAACGTCGTA‑TCACCCGCCGCCGGGGCAAACGGGGAAATCTT‑CAACAACCACCACGCGGTCTGCCTGGGAAACGAAAAAAGCGCGGAGAACGTCGTccc           >  1:177925/1‑250 (MQ=255)                                                                                                                                                                                                                                   ccGCAGACTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCCACAGATAAAATCGTCGTA‑TCACCAGCCGCCGGGACAATAGTGAAAAACTT‑CAACACCAACCACGCGTTCTGCCTGGAAACAGAAAAAGGCGCGGGGATAGTCGTCCATATggggat  >  1:26196/1‑248 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       CACCCGTCTGCGCCTTACAGTGGCTGACTCTGCCCGCGTTAACGATACGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGAAAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCGGCAACTGCCGCGCCTGTAGCAAAACCGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCGCCGATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTA‑TCACCAGCCGCAGGGACAATCGTGAAAATCTT‑CAACACCAACCACGCGTTCTGCCTGGAAACCGAAAAAGGCGCGGAGATCGTCGTCCATATGGGTAT  >  NC_000913/705182‑705658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑