Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,970	G→A	G129R (GGG→AGG)	glxR →	tartronate semialdehyde reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,970	0	G	A	100.0%	108.3 / NA	33	G129R (GGG→AGG)	glxR	tartronate semialdehyde reductase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/15);  total (18/15)

GAAACTGCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCTCAGGTTGAAGAAGTTCTGTTCGGTGAAAATGGTTGTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTGATGGGCGGC  >  NC_000913/536724‑537206                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             gAAACTGCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCTCAGGTTGAAGAAGTTCTGTTCGGTGAAAATGGTTGTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGAc                                                                                                                                                                                                                                          >  1:67446/1‑251 (MQ=255)       gCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCTCAGGTTGAAGAAGTTCTGTTCGGTGAAAATGGTTGTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTg                                                                                                                                                                                                                                      <  1:133279/249‑1 (MQ=255)          cGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCTCAGGTTGAAGAAGTTCTGTTCGGTGAAAATGGTTGTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGAt                                                                                                                                                                                                                                 <  1:247598/251‑1 (MQ=255)              aGGTAACGGCAACATCGGAGATCATGTTTATTAAGGTGCAGGACACACCTCAGGTTGAAGAAGTTCTGTTCGGTGAAAATGGTTGTATCAAAGCCTCGCTGAAGGGCAAAGCTATTGTTGAGATGAGCTCGTTTTCCCCGATTGAAACTAGGCGTTTGGCTCGGGAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCACCGGCGGTGAAATCGGTGCGCGTGAAAGGACTATGCCGATTATg                                                                                                                                                                                                                             <  2:329519/251‑1 (MQ=255)                              ggACATCATTTTTATTATGGTGCCGGACACACCTCAGGTTGAAGAAGTTCTGTTCGGTGAAAATGGTTGTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGGGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGtgatga                                                                                                                                                                                                               >  1:10220/1‑249 (MQ=255)                                     atTTTTATTATGGTGCCGGACACACCTCAGGTTGAAGAAGTTCTGTTCGGTGAAAATGGTTGTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGAGGTTGGCGATTATGGTTGGCGGCGCTGAAGCGGAAtt                                                                                                                                                                                                      >  1:84011/1‑251 (MQ=255)                                                                cAGGTTGAAGAAGTTCTGTGCGGTGAAAATGGTTGTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGaa                                                                                                                                                                             <  1:257725/249‑1 (MQ=255)                                                                      gaagaaGTTCTGTGCGGTGAAAATGGTTGTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGTCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCgg                                                                                                                                                                     <  1:257423/251‑1 (MQ=255)                                                                       aagaagTTCTGTTCGGTGAAAATGGTTGTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGt                                                                                                                                                                    >  1:314647/1‑251 (MQ=255)                                                                                tGTTCGGTGAAAATGGTTGTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATc                                                                                                                                                           >  1:403197/1‑251 (MQ=255)                                                                                 gTTCGGTGAAAATGGTTGTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGGGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCa                                                                                                                                                          <  2:10220/251‑1 (MQ=255)                                                                                        gAAAATGGTTGGACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGGCTTAAACCGCTGTTTGAACTGGTCGGTAAAAAGATCACGCTCGt                                                                                                                                                   <  2:58290/251‑1 (MQ=255)                                                                                          aaaTGGTTGTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTgg                                                                                                                                                 >  1:128450/1‑251 (MQ=255)                                                                                                  gTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAAc                                                                                                                                         <  1:119085/251‑1 (MQ=255)                                                                                                              cGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCTGTGGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTTAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGACCGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAGCGGCGATGGTc                                                                                                                               <  2:174904/249‑1 (MQ=255)                                                                                                                  gaacggCAATACCATTGTTTATATGTTGTCCATTTCCCCGATTCAACCTAATCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCTATTCGCTAGTCTCCGGCGGGGAAATGGTTGCGCGTCAAAGGTCGTTGTCGATTATGGTTGGCGGTGATGTAGCGGTATTTGAACGTGTTAAGCCGCTGTTTGAGCTGCTCGGTAAAAATATCTTCCTCCTGGTCGGGAACGGCTATTGTCAAAc                                                                                                                           <  2:372160/245‑1 (MQ=255)                                                                                                                               aTTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGATTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCTCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTAGGGTTGGCGGTGATGAACCGGAATTTGAACGAGTTAAACCGCTGTTTGAAATGCTCGCCAAAATTAACACCCTCGTGGGCGTTAACGGGTATGAACATACCTGCAAAGTGGCaa                                                                                                             >  2:334944/1‑250 (MQ=255)                                                                                                                                        aagAGCTACATTTTCGCGATGGAAGCTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGAGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTTTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAACATATCACCCGCGTGGGCGGTAACGGGGATGGTCAAACCTGCAAAGTGGCAAATCAGATTa                                                                                                    <  2:134091/248‑1 (MQ=255)                                                                                                                                                 aTTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCTGTAACGGCGATGTTCAAACCTGCAAAGTGGCAATTCAGATTATCGTGGCGCt                                                                                          <  1:129576/251‑1 (MQ=255)                                                                                                                                                                aCTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGNggg                                                                           >  2:420531/1‑250 (MQ=255)                                                                                                                                                                   aaGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTc                                                                        >  2:378418/1‑251 (MQ=255)                                                                                                                                                                          tCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGcc                                                                 >  1:90289/1‑251 (MQ=255)                                                                                                                                                                              tCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGGGAAAGGACGTTGTCGATTATGGTTGGCGGAGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGGTTCTGAAGCCCTGc                                                             >  1:66662/1‑251 (MQ=255)                                                                                                                                                                                 tCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTAt                                                          >  1:117199/1‑251 (MQ=255)                                                                                                                                                                                                gggCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGCAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCGGTTTGAACTGCTCGGTAAAAATATAACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGATGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCg                                           >  1:293181/1‑251 (MQ=255)                                                                                                                                                                                                          tATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGATGGCGGTGATGAAGCGGCATTTGAACGTGTTAAAACGCTGTTTGAATTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGGCACACCTGCAAAGTGGCAAATCCGATTCTCGTGGCGCTCAATATTGAAGCGGTTTCGGAAGCCCTGCTATTTACTTCACAAGCCGGTGCGGaacc                                 >  2:240903/1‑248 (MQ=255)                                                                                                                                                                                                           aTCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGCAGCGGTTTCTGAAGCCATGCTCTTTGCTTCAAAAGCCGGTGCGGACCCg                                >  1:165255/1‑251 (MQ=255)                                                                                                                                                                                                             ctcGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGt                              >  1:315850/1‑251 (MQ=255)                                                                                                                                                                                                                      ccAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTgcg                     >  1:148211/1‑251 (MQ=255)                                                                                                                                                                                                                       cAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTgcgc                    >  1:76087/1‑251 (MQ=255)                                                                                                                                                                                                                                     gAAATCGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTGATgg       <  1:364302/250‑1 (MQ=255)                                                                                                                                                                                                                                     gAAATCGGTGAGAGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTGATggg      <  2:111498/251‑1 (MQ=255)                                                                                                                                                                                                                                          cGGTGCGCGTGAAAGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTGATGggcggc  <  1:138829/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             GAAACTGCTCGCCAGGTAACGGAAGCATCGGACATCATTTTTATTATGGTGCCGGACACACCTCAGGTTGAAGAAGTTCTGTTCGGTGAAAATGGTTGTACCAAAGCCTCGCTGAAGGGCAAAACCATTGTTGATATGAGCTCCATTTCCCCGATTGAAACTAAGCGTTTCGCTCGTCAGGTGAATGAACTGGGCGGCGATTATCTCGATGCGCCAGTCTCCGGCGGTGAAATCGGTGCGCGTGAAGGGACGTTGTCGATTATGGTTGGCGGTGATGAAGCGGTATTTGAACGTGTTAAACCGCTGTTTGAACTGCTCGGTAAAAATATCACCCTCGTGGGCGGTAACGGCGATGGTCAAACCTGCAAAGTGGCAAATCAGATTATCGTGGCGCTCAATATTGAAGCGGTTTCTGAAGCCCTGCTATTTGCTTCAAAAGCCGGTGCGGACCCGGTACGTGTGCGCCAGGCGCTGATGGGCGGC  >  NC_000913/536724‑537206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑