Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	740,865	G→A	E453E (GAG→GAA)	phr →	deoxyribodipyrimidine photolyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	740,865	0	G	A	100.0%	114.8 / NA	34	E453E (GAG→GAA)	phr	deoxyribodipyrimidine photolyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/14);  total (20/14)

GTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAGCACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATT  >  NC_000913/740627‑741109                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     gTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCg                                                                                                                                                                                                                                          >  1:75221/1‑251 (MQ=255)   gATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGAGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCg                                                                                                                                                                                                                                          >  2:16007/1‑249 (MQ=255)     gttGGCGGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTGTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCgaga                                                                                                                                                                                                                                       <  2:222156/249‑1 (MQ=255)           aGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACaa                                                                                                                                                                                                                                 >  1:348225/1‑250 (MQ=255)            gacAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAAATGCGCGCTGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAACGCAGCGAGAGTACAAAc                                                                                                                                                                                                                               >  2:285389/3‑251 (MQ=255)               aaTAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGtt                                                                                                                                                                                                                            >  1:277062/1‑251 (MQ=255)                aTAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTg                                                                                                                                                                                                                           <  1:2659/251‑1 (MQ=255)                           gggcAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTGTCACCCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGa                                                                                                                                                                                                                <  2:348225/250‑1 (MQ=255)                              cAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAggc                                                                                                                                                                                                             >  2:147885/1‑251 (MQ=255)                                          tCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGAGTATGAGGCGGCGCGGAAggg                                                                                                                                                                                                 >  1:388819/1‑251 (MQ=255)                                                     cGATGCAGCGCCGTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAAc                                                                                                                                                                                         >  2:316466/1‑248 (MQ=255)                                                      gATGCAGGGCCTTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCTCGGAAGGGGAAATAACAGAc                                                                                                                                                                                     <  2:277062/251‑1 (MQ=255)                                                                     tttcgtattttcAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCg                                                                                                                                                                       <  2:363138/250‑1 (MQ=255)                                                                        cgtattttcAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGcc                                                                                                                                                                   >  2:110850/1‑251 (MQ=255)                                                                             tttcAACCCGACAACCCAGGGCGAGCAATTTGATCATGACGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGTGCCCAg                                                                                                                                                                >  2:251129/1‑249 (MQ=255)                                                                                      gACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATgc                                                                                                                                                     >  1:246778/1‑251 (MQ=255)                                                                                         aaaccAGGTCGAGAAATGTGATAATGTGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGTGAAAGTGGTGCATGAGACGTGGAAGTTGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCATGCGATAGTCGAACTGATAGAAGCGATAGTTCATACGTTGGCAGCGTATGGGGGGGCTCGGAAGTTGAAGTACCGGACGCTTCAGGCGATCGAGTCCATATGGCGGATgcgg                                                                                                                                                   <  2:64010/247‑1 (MQ=255)                                                                                               gggCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCTGATGCGGCGTGAAc                                                                                                                                            <  2:2330/251‑1 (MQ=255)                                                                                                              tCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGTCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCt                                                                                                                              >  1:214896/1‑250 (MQ=255)                                                                                                                                ccGCCTGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTc                                                                                                            <  1:201385/250‑1 (MQ=255)                                                                                                                                ccGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTc                                                                                                            <  2:104297/250‑1 (MQ=255)                                                                                                                                  gCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAAt                                                                                                         <  2:342429/251‑1 (MQ=255)                                                                                                                                               ggAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGGGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACATACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGaaa                                                                                            <  1:251129/251‑1 (MQ=255)                                                                                                                                                  aCTGCGCGATGTGCCAGGGAAAGTGGTGCATGGGCAGTGGCCGAGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATAc                                                                                         >  1:386277/1‑251 (MQ=255)                                                                                                                                                      agcgatatgaaaaagAAAGTGGTGGAAGCGCCGTGGAAGCGGGCGCAGAAAGCCGGTCCGCCGCTGAATCACCCGAAACCGAAAGTCGCATAAAACGAAGCGCGAGGACACACGTTGGCCGCCTATTAAGCGCCGCGGAAAGGGAAATAAGAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTCCACCCCTGATCCAGCCTCCAAATTTGTGCACATTGAATAAATCGCAGGAAATACGt                                                                                       >  2:199206/15‑249 (MQ=255)                                                                                                                                                                      aaGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGAc                                                                         <  2:270979/247‑1 (MQ=255)                                                                                                                                                                      aaGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGAc                                                                         >  1:270979/1‑247 (MQ=255)                                                                                                                                                                          ggtgCATGAGCCGTGGAAGAGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGAcgcggca                                                                   >  1:392382/1‑246 (MQ=255)                                                                                                                                                                             gCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCGAGCGt                                                              >  1:413096/1‑251 (MQ=255)                                                                                                                                                                                  aGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGAGTCGCAt                                                         >  2:337220/1‑251 (MQ=255)                                                                                                                                                                                           aGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCg                                                >  2:134753/1‑251 (MQ=255)                                                                                                                                                                                                 cgcAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTg                                          <  2:299337/251‑1 (MQ=255)                                                                                                                                                                                                          cAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTaa                                 <  1:312187/251‑1 (MQ=255)                                                                                                                                                                                                                                         aGTCGAACACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAAtt  >  2:169267/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     GTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGACAACCCAGGGCGAGAAATTTGATCATGAGGGCGAGTTTATCCGCCAGTGGCTACCGGAACTGCGCGATGTGCCAGGGAAAGTGGTGCATGAGCCGTGGAAGTGGGCGCAGAAAGCAGGTGTGACGCTGGATTATCCGCAACCGATAGTCGAGCACAAAGAAGCGAGAGTACAAACGTTGGCAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATT  >  NC_000913/740627‑741109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑