Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,152,816	C→T	D105N (GAT→AAT)	yegL ←	IPR002035 domain‑containing protein YegL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,152,816	0	C	T	100.0%	158.9 / NA	46	D105N (GAT→AAT)	yegL	IPR002035 domain‑containing protein YegL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/20);  total (26/20)

ACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCC  >  NC_000913/2152571‑2153051                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            aCGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAggg                                                                                                                                                                                                                                        >  1:191949/1‑251 (MQ=255)   ggAATTGTAATCCTTGCAGAGGCAAAGGCTGCCGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCAACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGTTAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCt                                                                                                                                                                                                                                      >  1:406973/1‑251 (MQ=255)    gAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCtt                                                                                                                                                                                                                                     >  2:97770/1‑251 (MQ=255)            aTCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGCTGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGAGACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATg                                                                                                                                                                                                                             >  2:107729/1‑251 (MQ=255)              ccTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGCTAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATCTTCAGGGATTTGGTAATGGc                                                                                                                                                                                                                           >  2:102958/1‑251 (MQ=255)                 tGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTTCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCg                                                                                                                                                                                                                         <  1:56479/250‑1 (MQ=255)                  gCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGc                                                                                                                                                                                                                        <  2:153918/250‑1 (MQ=255)                         cAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTg                                                                                                                                                                                                                <  1:199817/251‑1 (MQ=255)                                       cGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTgg                                                                                                                                                                                                  <  2:115460/251‑1 (MQ=255)                                            aTTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCaa                                                                                                                                                                                              >  2:305449/1‑250 (MQ=255)                                                     atcGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACTGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGTCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATggg                                                                                                                                                                                    <  1:262416/249‑1 (MQ=255)                                                     aGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATggg                                                                                                                                                                                    <  1:294400/251‑1 (MQ=255)                                                                       cccTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAgcggc                                                                                                                                                                  <  1:317113/251‑1 (MQ=255)                                                                         cTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGgcgc                                                                                                                                                                >  2:361376/1‑251 (MQ=255)                                                                                ccGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTaa                                                                                                                                                          >  1:198362/1‑250 (MQ=255)                                                                                             aagCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCcac                                                                                                                                             >  1:62905/1‑250 (MQ=255)                                                                                                cAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCATGCCACTCACCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGAAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGATGTTCCACATGc                                                                                                                                         >  1:50517/1‑251 (MQ=255)                                                                                                 aaaCCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCCTCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGc                                                                                                                                         >  2:63362/1‑250 (MQ=255)                                                                                                   aCCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCAcc                                                                                                                                      >  1:158170/1‑251 (MQ=255)                                                                                                         tATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTAATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCACTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCg                                                                                                                                >  1:312076/1‑251 (MQ=255)                                                                                                                    ccccGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCGCATGCACCGGGCCGAACGTCACAAt                                                                                                                     <  1:361376/251‑1 (MQ=255)                                                                                                                       cGAAACACTTTGTTGGCTGCAGCCTTCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCCCCCATTGGTGTAGCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTTTTCCACATGCACCGGGCCGAACGTCACAATGcc                                                                                                                  <  1:350689/251‑1 (MQ=255)                                                                                                                         aaaCACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGACGCACCCATTGGTGTATCGCCATGGGCAAAAAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGcgg                                                                                                                 >  2:144617/1‑248 (MQ=255)                                                                                                                             acTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTc                                                                                                            >  1:254989/1‑251 (MQ=255)                                                                                                                                    tgGCTGCAGCCTGACACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCtt                                                                                                     >  2:222247/1‑251 (MQ=255)                                                                                                                                             ccTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGcc                                                                                            <  1:305449/251‑1 (MQ=255)                                                                                                                                              cTGCCACTCATCGGCTGGTGCCCCATCGGTAATCAGGAAAATCCACGGGCGCTAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAACAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAANcc                                                                                            >  2:406445/1‑250 (MQ=255)                                                                                                                                                      cATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCa                                                                                   <  2:375333/251‑1 (MQ=255)                                                                                                                                                         cGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCagc                                                                                 >  1:335156/1‑250 (MQ=255)                                                                                                                                                                        cGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACg                                                                  <  1:78706/250‑1 (MQ=255)                                                                                                                                                                              tCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGt                                                            >  1:258022/1‑250 (MQ=255)                                                                                                                                                                                        ccANGGTCGATAATACGAAATACCATTTGCACGATACTCGCGTTTCCGCTCCTCTACCATATTCAGGGCTTGGGTAATAGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGTGTTGTTCCACATGCACCGGTCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAACCCAGCAGATCAGCAAGCAGTTCAGCACGAAAGGTAACCAATCCgg                                                 <  2:406973/251‑1 (MQ=255)                                                                                                                                                                                                gATAATACGAAATACCATTTGCACGATACTCTCGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGt                                         <  2:48460/251‑1 (MQ=255)                                                                                                                                                                                                      aCGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTa                                   <  1:240075/251‑1 (MQ=255)                                                                                                                                                                                                         aaaTACCATTTGCACGATACTCACGTTTCCGCTCCTCTCCCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGACAGGTAACCAATCCGGCGTTAAGTTCATTAAt                                 >  2:280522/1‑250 (MQ=255)                                                                                                                                                                                                             aCCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTaa                                  >  1:217426/1‑245 (MQ=255)                                                                                                                                                                                                             aCCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTaa                                  <  2:217426/245‑1 (MQ=255)                                                                                                                                                                                                             aCCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTc                            >  1:254926/1‑251 (MQ=255)                                                                                                                                                                                                                  ttGCACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTAcc                        <  1:97770/250‑1 (MQ=255)                                                                                                                                                                                                                     cACGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTc                    >  2:329890/1‑251 (MQ=255)                                                                                                                                                                                                                      aCGATACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTc                    >  1:365987/1‑250 (MQ=255)                                                                                                                                                                                                                         aTACTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGAGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCAGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGCAAGGTAACCAATCAGGCGTTAAGTTCATTAATAGATCTACCGTTCAt                  >  1:91155/1‑249 (MQ=255)                                                                                                                                                                                                                           aCTCACGTTTCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGa               >  2:125567/1‑250 (MQ=255)                                                                                                                                                                                                                                   ttCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGaaa       <  2:11455/250‑1 (MQ=255)                                                                                                                                                                                                                                   ttCCGCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGaaa       <  1:321329/250‑1 (MQ=255)                                                                                                                                                                                                                                       gCTCCTCTACCATATTCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATcc  <  2:258022/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            ACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCACCATCGGTAATCAGGAAAATCCACGGGCGATAATACGAAATACCATTTGCACGATACTCACGTTTCCGCTCCTCTACCATATCCAGGGCTTTGGTAATGGCCGCACCCATTGGTGTATCGCCCTGGGCAAACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCC  >  NC_000913/2152571‑2153051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑