Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,122,452	G→A	T52T (ACC→ACT)	pyrC ←	dihydroorotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,122,452	0	G	A	100.0%	147.7 / NA	44	T52T (ACC→ACT)	pyrC	dihydroorotase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/28);  total (16/28)

GATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACGGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGTTTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGA  >  NC_000913/1122209‑1122682                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       gATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCa                                                                                                                                                                                                                                 <  1:157713/251‑1 (MQ=255)  aTTTTTTCCATGCGCTCAAGTACCGGCATGATTTCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCACCAGTGGTCAc                                                                                                                                                                                                                                <  1:399174/251‑1 (MQ=255)         ccATGCGCTCAATTACCGGCATGAGTGCGTCAATTGCCGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGGCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGa                                                                                                                                                                                                                         <  1:185607/251‑1 (MQ=255)         ccATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCgg                                                                                                                                                                                                                          <  1:99755/250‑1 (MQ=255)         ccATGCACTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCCGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCCACGAATCTGTTAAATAACAGGCCATCAAGGGGGTGAAATCGTGCCCGGCAGGCACGGCGTCAAGAATACGCTGGCGGTACGCCACGGCAGCTTAAACAGGGGGCACGGGCgg                                                                                                                                                                                                                          >  2:425681/1‑250 (MQ=255)           aggcgcTCAAGTACCGGCATGAGTGCGTCAATTGACTTCACGCCGTGGCTGGAGGTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCATCGGTGAACACGCCTACGTTAAATCCGCGCTCCAGCTCATTAGTATCCAGCGAATCTGGTAAATAACAGTTCATCAATGGGGTGAAATCGAGCCCGGCAGGTATGGCGTTAAGGAGACGCTGGCGATACGCCACGGCAGTCTCAACAGTGGTCACGGGCGGAGc                                                                                                                                                                                                                       <  1:434632/249‑1 (MQ=255)           aTGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCAAGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCCCGGGCGGAGc                                                                                                                                                                                                                       >  1:25638/1‑251 (MQ=255)                  cAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGAtt                                                                                                                                                                                                                 <  1:80507/250‑1 (MQ=255)                                aTTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGTTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGc                                                                                                                                                                                                  <  2:341349/251‑1 (MQ=255)                                   gCGTCAATTGACGTCACGCCGGGGCTGGAGGTAGTGGTTGCGTGTGCCGGGTAAAGTTTGGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATGAGGCTCCAGCGAACCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAACACGCTGGCGATACGCCCCGGCATCCTCAACAGTGGTCCCGGGCGGCGCCAGATTGGTCATTACGATAGccc                                                                                                                                                                                                <  2:433308/250‑1 (MQ=255)                                         aTTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTc                                                                                                                                                                                                                                  <  2:292892/210‑1 (MQ=255)                                         aTTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTc                                                                                                                                                                                                                                  >  1:292892/1‑210 (MQ=255)                                                         ggCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGtata                                                                                                                                                                          <  1:30428/250‑1 (MQ=255)                                                             ggAGTTAGTGGTTGCGTTTGCCGTGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCTATACGCCACGGCAGCCTCAACAGTGTTCACGGGCGGTGCCAGATTGTGCATTACGATAGCCCTTCCATAAATTTCGCTGGTATCTGGc                                                                                                                                                                      <  2:13571/250‑1 (MQ=255)                                                              gAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCAc                                                                                                                                                                    <  1:437689/251‑1 (MQ=255)                                                                gTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACg                                                                                                                                                                   >  1:97455/1‑250 (MQ=255)                                                                  tAGTGGTTGCGTTTGCCGGGTAAAGTGTGGGGGCAGTGAACACGCCTTCGTTAAATCCGCGCGCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGAGCGGCAGGTACGGGGTCAAGAATACGCTGGCGATACGCCACGGCAGCCACAACAGTAGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTACATAAACTTCGCGGGTATATGGCACGAc                                                                                                                                                                 <  2:219735/250‑1 (MQ=255)                                                                      ggTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAACTTTCGCTGGTATATGGCACGACAGttt                                                                                                                                                            >  2:87259/1‑251 (MQ=255)                                                                         tGCGGTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTAGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGttttt                                                                                                                                                          <  1:68787/250‑1 (MQ=255)                                                                              ttGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACAt                                                                                                                                                     <  2:49297/250‑1 (MQ=255)                                                                              ttGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATg                                                                                                                                                    >  1:197584/1‑251 (MQ=255)                                                                                gCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTc                                                                                                                                                  <  1:379474/251‑1 (MQ=255)                                                                                                           actccTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTg                                                                                                                             >  2:20004/4‑245 (MQ=255)                                                                                                           actccTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTg                                                                                                                             <  1:20004/242‑1 (MQ=255)                                                                                                           acGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAgtc                                                                                                                       <  1:419429/251‑1 (MQ=255)                                                                                                                 tCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACATGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATACCCTGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCTCGGAGGTGACGGTGCCAGTCGTCt                                                                                                                   <  2:325389/249‑1 (MQ=255)                                                                                                                        aTCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCAGCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGcggcgg                                                                                                          <  2:320605/251‑1 (MQ=255)                                                                                                                                tCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTAACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTa                                                                                                   >  1:242376/1‑250 (MQ=255)                                                                                                                                      tCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCAGGCAGGTACGGATTCAAGAATACGCTGGCGATACGCAACGGCAGCCTCAACAGGGGTCACGGGCGGAGACAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGGGCCAGTAGTCTGGGCGGCGGATCTTTAATACCTg                                                                                            >  1:34691/1‑251 (MQ=255)                                                                                                                                       gataaGGATCCAGCGAATCTGTTAAATCACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATGCGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCTCGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTGTAATACCt                                                                                             <  2:97455/245‑1 (MQ=255)                                                                                                                                       cATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTgg                                                                                           <  1:137340/251‑1 (MQ=255)                                                                                                                                            ggATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATgg                                                                                      >  1:435321/1‑251 (MQ=255)                                                                                                                                                   gCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTc                                                                               <  1:49012/251‑1 (MQ=255)                                                                                                                                                   gCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTc                                                                               >  2:434667/1‑251 (MQ=255)                                                                                                                                                           gTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTAAAGAATACGCTGGCGATACGCCACTGCAGCCTCAACAGTTGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTAAAGGTGCCAGTCGTCGGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTa                                                                           <  1:341720/247‑1 (MQ=255)                                                                                                                                                                 tAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGACATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGCGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGc                                                                 >  2:116002/1‑251 (MQ=255)                                                                                                                                                                  aaCAGGTCATCAATGGGGTTAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGCCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGATAGTTTTTAACATGTCGCCATCGCGGAGGTGAATGTGCCAGTCTTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCt                                                                <  1:304542/251‑1 (MQ=255)                                                                                                                                                                                      aaaTCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCCACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGttttt                                            >  1:287646/1‑251 (MQ=255)                                                                                                                                                                                           gTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGTTTTTGCCgg                                       >  2:383419/1‑251 (MQ=255)                                                                                                                                                                                           gTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGTTTTTGCCgg                                       <  1:181969/251‑1 (MQ=255)                                                                                                                                                                                             gCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGTTTTTGCCGGac                                     <  1:109640/251‑1 (MQ=255)                                                                                                                                                                                                    aGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGTTTTTGCCGGACACAAAgg                              >  1:336186/1‑251 (MQ=255)                                                                                                                                                                                                         cGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGTTTTTGCCGGACACAAAGGATAAg                         <  1:27979/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCGCCACGGCAGCCTCAACAGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCGTTAATCTCTATGCTCCGGCTGAAGGGATGTTTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGa  >  1:350047/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       GATTTTTTCCATGCGCTCAAGTACCGGCATGATTGCGTCAATTGACGTCACGCCGTGGCTGGAGTTAGTGGTTGCGTTTGCCGGGTAAAGTTTTGCAGCGGTGAACACGCCTTCGTTAAATCCGCGCTCCAGCTCATTAGGATCCAGCGAATCTGTTAAATAACAGGTCATCAATGGGGTGAAATCGTGCCCGGCAGGTACGGCGTCAAGAATACGCTGGCGATACGCCACGGCAGCCTCAACGGTGGTCACGGGCGGAGCCAGATTGGGCATTACGATAGCCCGTCCATAAATTTCGCTGGTATATGGCACGACAGTTTTTAACATGTCGCCATCGCGGAGGTGAAGGTGCCAGTCGTCTGGGCGGCGGATCTTTAATACCTGGGATGGTGCAGTCATTAATCTCTATGCTCCGGCTGAAGGGATGTTTTTGCCGGACACAAAGGATAAGCGGAAACGTTTTCCTTTGCACGA  >  NC_000913/1122209‑1122682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑