Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,204,319	T→C	intergenic (+30/‑277)	yehK → / → yehL	uncharacterized protein YehK/putative AAA(+) MoxR family ATPase YehL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,204,319	0	T	C	100.0%	48.9 / NA	15	intergenic (+30/‑277)	yehK/yehL	uncharacterized protein YehK/putative AAA(+) MoxR family ATPase YehL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/9);  total (6/9)

CCGTCTATCGACTGGAGCGATTCCGGCTACGTCCAACATTTCATCAACGTGATCGAGAAGATGCTGACCCGCTAATAAATGATGCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGC  >  NC_000913/2204084‑2204551                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         ccGTCTATCGACTGGAGCGATTCCGGCTACGTCCAACATTTCATCAACGTGATCGAGAAGATGCTGACCCGCTAATAAATGATGCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGACCCGGCGCAACGTccc                                                                                                                                                                                                                           <  1:401341/251‑1 (MQ=255)   gTCTATCGACTGGAGCGATTCCGGCTACGTCCAACATTTCATCAACGTGATCGAGAAGATGCTGACCCGCTAATAAATGATGCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGACCCGGCGCAACGTCCCaa                                                                                                                                                                                                                         <  1:160207/251‑1 (MQ=255)    tCTATCGACTGGAGCGATTCCGGCTACGTCCAACATTTCATCAACGTGATCGAGAAGATGCTGACCCGCTAATAAATGATGCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGACCCGGCGCAACGTCCCAAt                                                                                                                                                                                                                        >  2:349447/1‑251 (MQ=255)                                  cAACATTTCATCAACGTGATCGAGAAGATGCTGACCCGCTAATAAATGATGCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGACCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAAt                                                                                                                                                                                          <  1:62723/251‑1 (MQ=255)                                                                          aataaATGATGCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGACCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTgg                                                                                                                                                   >  1:316558/1‑250 (MQ=255)                                                                                         gATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGACCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGcac                                                                                                                                    >  2:343979/1‑250 (MQ=255)                                                                                            ttATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGACCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAgg                                                                                                                                 <  1:280238/250‑1 (MQ=255)                                                                                                     agaTGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGACCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGtt                                                                                                                       >  1:265730/1‑251 (MQ=255)                                                                                                           gtgtAGTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGACCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCt                                                                                                                 <  1:70635/251‑1 (MQ=255)                                                                                                               aTTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGACCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATGCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGaaa                                                                                                             <  1:194691/251‑1 (MQ=255)                                                                                                                                             ccTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGACCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCAGTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACcagc                                                                               <  2:315696/251‑1 (MQ=255)                                                                                                                                                        aaTAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATGCAAATTGGTCCGACCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGttttttt                                                                     <  1:448723/250‑1 (MQ=255)                                                                                                                                                                                       atatTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGACCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATAttttt                                      <  1:376449/250‑1 (MQ=255)                                                                                                                                                                                                   gAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGACCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACt                         >  1:412621/1‑251 (MQ=255)                                                                                                                                                                                                                          taatTCAAATTGGTCCGACCCGGCGAAACGTCCCAATGGCCTGGATAATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTTGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGAATTTTTTCGCTAAAAAAACTAATGCGCCAACCATTTAttccgg  >  1:292794/1‑247 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         CCGTCTATCGACTGGAGCGATTCCGGCTACGTCCAACATTTCATCAACGTGATCGAGAAGATGCTGACCCGCTAATAAATGATGCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGC  >  NC_000913/2204084‑2204551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑