Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,275,757	G→A	G162E (GGG→GAG)	yejE →	putative oligopeptide ABC transporter membrane subunit YejE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,275,757	0	G	A	100.0%	127.9 / NA	38	G162E (GGG→GAG)	yejE	putative oligopeptide ABC transporter membrane subunit YejE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/24);  total (14/24)

GACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGTCA  >  NC_000913/2275510‑2276002                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                          gtcccGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGgc                                                                                                                                                                                                                                                        <  1:88564/249‑1 (MQ=255)           tGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTCTCAACTTTGATACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCCGCGCTTTGCTGGCACGCATTCTCTTTGGCGCGCGGAGCCCGGTTCTGTTTGGGCCGGGGCTGACTCTCTGTTCCCGCGTGATGGGTGTGCTGGCGGAGGCGCTACCAGGc                                                                                                                                                                                                                                              >  2:274196/1‑251 (MQ=255)                          aaaaTAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTaaa                                                                                                                                                                                                                               <  2:107490/251‑1 (MQ=255)                                   gCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGtgacaacgttgcc                                                                                                                                                                                                                      >  2:277126/1‑239 (MQ=255)                                   gCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTaaaagcgagctc                                                                                                                                                                                                                      >  1:145690/1‑242 (MQ=255)                                             gtgGGCACCGATTCGCGTTGGTGCTACCAGTATCAACGTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTTCGGCGGTAAAGTC‑GATCTCT‑GGGGACaa                                                                                                                                                                                                           <  2:260877/250‑1 (MQ=255)                                               gggCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAATGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACg                                                                                                                                                                                                         <  1:165174/250‑1 (MQ=255)                                                       aTTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGGAAAATC‑GATCTCT‑gggggaaaagctttttttg                                                                                                                                                                                                >  1:244718/1‑236 (MQ=255)                                                              ttGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAAGC‑GATCTCT‑TGGGGCAACGC‑CTTTTTTAAGTATgg                                                                                                                                                                                        >  1:184637/1‑251 (MQ=255)                                                                   gCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTAt                                                                                                                                                                                          <  2:53764/244‑1 (MQ=255)                                                                        cAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GCTCTCT‑GGGGGCAACGC‑CTTATTTTAGTATgggtgggggggc                                                                                                                                                                              >  1:162777/1‑241 (MQ=255)                                                                         aGTATCACCTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑cc                                                                                                                                                                            <  2:32365/251‑1 (MQ=255)                                                                            aTCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGAc                                                                                                                                                                         <  2:184637/251‑1 (MQ=255)                                                                               aaCTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCt                                                                                                                                                                      <  1:130569/251‑1 (MQ=255)                                                                                   ttGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGt                                                                                                                                                                    <  1:377261/249‑1 (MQ=255)                                                                                   ttGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGGAAAGGC‑TATCTCT‑TGGGGCAACAC‑CTTATTTGAGGATGGGCCGGGATG‑ccagcgccgttt                                                                                                                                                                  >  1:260138/1‑241 (MQ=255)                                                                                    tGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGttt                                                                                                                                                                  <  1:12224/250‑1 (MQ=255)                                                                                                         ttcTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTTCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATTTCT‑TGGGGGAACGC‑TTTTTTTTAGTATGGTCGGGGGTG‑CCGACGCTGTTTTTTATTATTTTTCtttttcgct                                                                                                                                            >  1:274363/1‑245 (MQ=255)                                                                                                          tctcCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGCTAAGTCTGA‑CTCT‑GGGGGCAAAGCCTTTTTTGAAGT‑TGGGTGGGGGTG‑CCGACGGTGttttttgtttctttttcttcccgagg                                                                                                                                           >  1:25513/1‑230 (MQ=255)                                                                                                                  cTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTT‑GCTATCT‑GGGGACAAAGC‑TTTATTGTAGTATTGTCGGGGATGTCCAAC‑CTGTTTTTGTTTTTTTTAttttttcgcgttctaaagc                                                                                                                                   >  1:53174/1‑236 (MQ=255)                                                                                                                        gCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACt                                                                                                                             <  1:414073/251‑1 (MQ=255)                                                                                                                          aaaaCTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTc                                                                                                                           <  1:43031/251‑1 (MQ=255)                                                                                                                                ggctggGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCtggtg                                                                                                                      <  2:274363/250‑1 (MQ=255)                                                                                                                                ggctggGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCtggt                                                                                                                       <  1:406265/249‑1 (MQ=255)                                                                                                                                  gtggGAACGGATGCCCACGGCGGCTGTGTGCTGGCACGCATTCTCTAGGGCACGCGGATCTCGGTTCTGTTTGGCCTTATGCTGACTCTCGGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTAGTTTACTTTCCAGCGTCGTACAGCCTAACTGCTGGTGgct                                                                                                                   <  2:311267/250‑1 (MQ=255)                                                                                                                                      gAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCTTGGGGCAAACCC‑TTT‑TTGGAGTAAGGTCGGGGATG‑CCGCCGCTGTTTTTTTTTATTTTTCTTTTCCGCCGCGTACAGCCTAACTTCTGGGGGgtgctg                                                                                                               >  1:356585/1‑251 (MQ=255)                                                                                                                                                cAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAAAC‑GATCTCT‑GGGGACAACGC‑CTTTTTGGAGTATGGTCGGGGAGG‑CCGACGCTGTTTTTTTTTATTTTTCTTTTCCGCGTCGTTCAGCCTAACTTCTGGTGGTTTCTTGCAAtttctt                                                                                                     >  1:396167/1‑247 (MQ=255)                                                                                                                                                                  ggCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGa                                                                                   <  1:361308/251‑1 (MQ=255)                                                                                                                                                                     ccGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAGGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTGGTTTGTCTGGATGAGTc                                                                                <  2:448153/250‑1 (MQ=255)                                                                                                                                                                     aCGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTc                                                                                <  2:131589/251‑1 (MQ=255)                                                                                                                                                                       gCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑TGGGGCAACGC‑TTTATTTAAGTATGGTCGGGGGTG‑CCGAc                                                                                                                                                                         >  1:430071/1‑160 (MQ=255)                                                                                                                                                                       gCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGAc                                                                                                                                                                         <  2:430071/160‑1 (MQ=255)                                                                                                                                                                                  ggCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTCGGCgtgg                                                                    <  1:255711/250‑1 (MQ=255)                                                                                                                                                                                         ggATCTCGGTTCTGTTTGGCCGGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTCGGCGTGGTgcgggcg                                                            <  2:244718/251‑1 (MQ=255)                                                                                                                                                                                                                        tctctGTTCCAGCGTGATGGGCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGAC‑GCTCTCT‑GGGGGCAACGC‑TTTATTGAAGTATGGGCGGGGAGG‑CCGACGCTGTTTTTGATTTTTTTTCTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGGTGGGTGAGTCTGGTCGGCGTGGGGCGGGCGGGGGTTTTTCGTACTCGTAATTTCGACtta                              >  1:179158/1‑248 (MQ=255)                                                                                                                                                                                                                                            gCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCtt         <  1:138339/251‑1 (MQ=255)                                                                                                                                                                                                                                            gCGTGCTGGCGGAGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTACTCGTAATTTCGACTACATGCGTGCGGCACAGGCGCtt         <  1:140304/251‑1 (MQ=255)                                                                                                                                                                                                                                                   ggCGGAGGCGCTACAAGGCTATTACGTCGGTAAAGTC‑GATCTCT‑TGGGACAACGC‑TTTATTGAATTATGGTCGTGTATG‑CCGACGCTGTTTTTGATTATTTTACTGTCCATCGTCTTACAGCCTATCTTCTGGTTGCTGCTGGCAATTACTGTCTTGTTTGTCTGGATGAGTCTGGTCGGCGTTGTGCGGGCGGAGTTTTTACGTACTCGTAATTGCTACTACATTCGTGCGGCACAGGCGCTTGGCGTCa  <  1:388559/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                          GACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGACTCTCTGTTCCAGCGTGATGGGCGTGCTGGCGGGGGCGCTACAAGGCTATTACGGCGGTAAAGTC‑GATCTCT‑GGGGACAACGC‑TTTATTGAAGTATGGTCGGGGATG‑CCGACGCTGTTTTTGATTATTTTACTTTCCAGCGTCGTACAGCCTAACTTCTGGTGGCTGCTGGCAATTACTGTCTTGTTTGGCTGGATGAGTCTGGTCGGCGTGGTGCGGGCGGAGTTTTTACGTACTCGTAATTTCGACTACATTCGTGCGGCACAGGCGCTTGGCGTCA  >  NC_000913/2275510‑2276002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑