Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,586,071	G→A	I251I (ATC→ATT)	ggt ←	glutathione hydrolase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,586,071	0	G	A	98.0%	165.8 / NA	49	I251I (ATC→ATT)	ggt	glutathione hydrolase proenzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (28/20);  minor base C (1/0);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCGATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCTTTACTGTTTTCGTGATTCGGCAACACTTCGCTACCGTAGGTTTTGAGATCGTCAGCCAGCGCGTCGTTAACGATAAAACCATCGCGTGCCAGTTTAAACGCGGGCT  >  NC_000913/3585824‑3586312                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  aggcgtaTTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATc                                                                                                                                                                                                                                                >  1:132488/1‑251 (MQ=255)   gcgtaTTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTg                                                                                                                                                                                                                                              >  1:136527/1‑251 (MQ=255)     gtaTTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCGTGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGtt                                                                                                                                                                                                                                            >  1:139903/1‑251 (MQ=255)             ccGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTATTTTTTCATATCGAAGTTTTCCAGAATATGGAGGATGTGTACGACATGGATCCCGCCGGAGGATGGTGGTGGCATGTAGTAAACCTGATACCCGCTATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTcctca                                                                                                                                                                                                                                       <  2:114496/248‑4 (MQ=255)               gCTTCTGCCATGATTTGCCTCGCATCGGCGCTGCCAAAGCCGTATTTCTTCATATCGAAGTTTTCCCGAGTATTCAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTTATACCCGCGATAATCCCCGCTTATCGGAGGGCGTTCGAACTCCTTATAGGCTACTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGt                                                                                                                                                                                                                                  >  2:152096/1‑251 (MQ=255)                cTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGCATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTg                                                                                                                                                                                                                                 >  1:374720/1‑251 (MQ=255)                    tGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCtt                                                                                                                                                                                                                             >  2:40174/1‑251 (MQ=255)                        aTGATTTGCATCGCATCGGCGCTGCCAAAGCCGTATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTtata                                                                                                                                                                                                                         <  1:444446/251‑1 (MQ=255)                                    gcatcgGCGCTGCCAAAGCCGTATTTCTTCATATCGAAGTGTTCCAGAATATTGAGGATTTGTACGAGATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTACGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCTTGCCTTTATAGAATTCGTCCgg                                                                                                                                                                                                             <  2:426370/251‑1 (MQ=255)                                     catcgGCGCTGCCAAAGCCGTATTTCTTCATTGCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCgtt                                                                                                                                                                                                            <  2:331366/251‑3 (MQ=255)                                          gcgcTGCCAAAGCCGTATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCNGtt                                                                                                                                                                                                       >  2:420667/1‑251 (MQ=255)                                           cgcTGCCAAAGCCGTATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTcc                                                                                                                                                                                                                                          <  2:7380/215‑1 (MQ=255)                                           cgcTGCCAAAGCCGTATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTcc                                                                                                                                                                                                                                          >  1:7380/1‑215 (MQ=255)                                                 cAAAGCCGTATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCACTCTGTTCCGCAATCGTGCCTTTTTCGAATTCTTCCGGGCCGttttgatcg                                                                                                                                                                                                >  2:458226/1‑246 (MQ=255)                                                 cAAAGCCGTATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGttt                                                                                                                                                                                                      <  1:458226/245‑1 (MQ=255)                                                        gTATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCAtct                                                                                                                                                                                         <  1:242723/251‑1 (MQ=255)                                                                tcATATCGAAGTTTTCCAGAATATTGTGGATTTGTACGATATGGATCCCGCCGGAGGATTGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAATTCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGTGCAATCTTTTCCTCAAGCGTTCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCATTCATCTCCAGGc                                                                                                                                                                                   <  2:297948/249‑1 (MQ=255)                                                                 cATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGctct                                                                                                                                                                                <  1:40174/251‑1 (MQ=255)                                                                   tatCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGCTCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCttt                                                                                                                                                                              >  2:96875/1‑251 (MQ=255)                                                                    atCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTg                                                                                                                                                                             <  1:158569/251‑1 (MQ=255)                                                                         aGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGcc                                                                                                                                                                                                          >  1:416492/1‑217 (MQ=255)                                                                         aGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGcc                                                                                                                                                                                                          <  2:416492/217‑1 (MQ=255)                                                                             ttCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTc                                                                                                                                                                     <  2:115458/250‑1 (MQ=255)                                                                                    atatTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCAc                                                                                                                                                             >  2:454569/1‑251 (MQ=255)                                                                                                tGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGcc                                                                                                                                                 >  2:266928/1‑251 (MQ=255)                                                                                                   aCGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCt                                                                                                                                               <  1:229156/250‑1 (MQ=255)                                                                                                           gATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCg                                                                                                                                      >  1:386296/1‑251 (MQ=255)                                                                                                             ccccGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGc                                                                                                                                    <  2:395434/250‑1 (MQ=255)                                                                                                                  ccgGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGcc                                                                                                                               >  2:317301/1‑251 (MQ=255)                                                                                                                        gaTGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCttt                                                                                                                         <  1:326603/251‑1 (MQ=255)                                                                                                                                    aTGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGc                                                                                                             <  1:60439/251‑1 (MQ=255)                                                                                                                                       gAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTTGACAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCttt                                                                                                          >  1:451514/1‑251 (MQ=255)                                                                                                                                        aGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCtttt                                                                                                         >  1:417995/1‑250 (MQ=255)                                                                                                                                          tAAACCTGAAACCCGCGCTAATCGCAGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTCAATCTTCTTTAGCGATCAAGCCACCGCTTTTCTGCCTCTCCTGTGCAATCTGGTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTGCGCGGCTCGCCCTCTTTTCATCAGAAAGCTTTACt                                                                                                       >  2:320966/1‑251 (MQ=255)                                                                                                                                                       cgcgATAATCGGCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCATAAGATAGCTTTACTGTTTTCGTTATTc                                                                                          <  2:32344/251‑1 (MQ=255)                                                                                                                                                                          cGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCTTTACTGTTTTCGTGATTCGGCAACACTTCGCTACCGt                                                                       >  2:160945/1‑251 (MQ=255)                                                                                                                                                                              gTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCTTTACTGTTTTCGTGATTCGGCAACACTTCGCTACCGTAGGt                                                                   >  2:37124/1‑251 (MQ=255)                                                                                                                                                                                  gTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCTTTACTGTTTTCGTGATTCGGCAACCCTTCGCTACCGTCGGttttt                                                               >  1:255145/1‑250 (MQ=255)                                                                                                                                                                                          gCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCTTTACTGTTTTCGTGATTCGGCAACACTTCGCTACCGTAGGTTTTGAGATCGTc                                                       >  1:119381/1‑251 (MQ=255)                                                                                                                                                                                                     tgctAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTGGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCTTTACTGTTTTCGTGATTCGGCAACACTTCGCTACCGTAGGTTTTGAGATCGTCAGCCAGCGcgt                                            >  1:211104/1‑251 (MQ=255)                                                                                                                                                                                                      gctAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGACGATAGCTTTACTGTTTTCGTGATTCGGCACCACTTCGCTACCTTCGGTTTTGTGATCGTCAGACAGCGcgtc                                           >  2:80698/1‑251 (MQ=255)                                                                                                                                                                                                              ttcttTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATAGTGCCTTTATAGAATTCGTACGGGCCGTTTTCATCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGAGTGTCGACCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCTTTACTGTTTTCGTGATTCGGAAACACTTCGCTACCGGAGGTTTTGAGATCGTCAGCCAGCGCGTCGTTAACg                                    >  1:419140/1‑250 (MQ=255)                                                                                                                                                                                                                             gCCACCGTTTTTCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCTTTACTGTTTTCGTGATTCTGCAACACTTCGCTACCGTAGGTTTTGAGATCGTCAGCCAGCGCGTCGTTAACGATCAAACCATCGCGTg                    >  2:117856/1‑251 (MQ=255)                                                                                                                                                                                                                                     ttttCGGCATCTCCTGGACAATCTGTTCCGCAATCGTGCCTTTATATAATTCGTCCGTGCCGTTTACATCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCTTTACTGTTTTCGTGATTCGGCAACACTTCGCTACCGTAGGTTTTGAGATCGTCAGCCAGCGCGTCGTTAACGATAAAACCATCGCGTGCCAGttt             <  2:337260/250‑1 (MQ=255)                                                                                                                                                                                                                                      tttCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCTTTACTGTTTTCGTGATTCGGCAACACTTCGCTACCGTAGGTTTTGAGATCGTCAGCCAGCGCGTCGTTAACGATAAAACCATCGCGTGCCAGTTTa            >  2:452062/1‑250 (MQ=255)                                                                                                                                                                                                                                       ttCTGCATCTCCTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCTTTACTGTTTTCGTGATTCGGCAACACTTCGCTACCGTAGGTTTTGAGATCGTCAGCCAGCGCGTCGTTAACGATAAAACCATCGCGTGCCAGTTTaa           <  1:107505/250‑1 (MQ=255)                                                                                                                                                                                                                                        tCTGCATTTCCTGCGCCATCTGTCCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTCTGCCCGGTTCGCCTGAACCAGCGTGTCGCCCTTTTTCAGCGGTTCGCCCTCCTTCCCGAAGACAGCTTTAATGTTTTCGTGCTTTGGCCACACTTCGCCACCGTAGGTTTTGGGATCGTCAGCCTGCGCGCCGTTAACGATAAAACCCTCGAGTGccctcttaaac         >  2:446111/1‑242 (MQ=255)                                                                                                                                                                                                                                              tctcCTGGGCAATCTGATCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCAGTTTTCAGAAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCATTTTTCAGCGGCTCGCACTCTTTCCAGAAGATAGCGTTAATGATTTCGTGATTCGGCAACAATTCGCTACCGTAGGTTTTGAGATCGTCAGCCAGCGCGTCGTTAACGATAAAACCATGGCGTGCCAGTTTAAAcgcaga    >  1:372044/1‑247 (MQ=255)                                                                                                                                                                                                                                                 ccTGGGCAATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCTTTACTGTTTTCGTGATTCGGCAACACTTCGCTACCGTAGGTTTTGAGATCGTCAGCCAGCGCGTCGTTAACGATAAAACCATCGCGTGCCAGTTTAAACGCGGGCt  <  2:136527/249‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  AGGCGTATTTCTCCGCTTCTGCCATGATTTGCATCGCATCGGCGCTGCCAAAGCCGTATTTCTTCATATCGAAGTTTTCCAGAATATTGAGGATTTGTACGATATGGATCCCGCCGGAGGATGGCGGTGGCATGGAGTAAACCTGATACCCGCGATAATCGCCGCTTATCGGAGTGCGTTCGACCGCTTTATAGGCTGCTAAATCTTCTTTAGTGATCAAGCCACCGTTTTTCTGCATCTCCTGGGCGATCTGTTCCGCAATCGTGCCTTTATAGAATTCGTCCGGGCCGTTTTCAGCAATCATCTCCAGGCTCTTTGCCAGGTTCGCCTGCACCAGCGTGTCGCCCTTTTTCAGCGGCTCGCCCTCTTTCCAGAAGATAGCTTTACTGTTTTCGTGATTCGGCAACACTTCGCTACCGTAGGTTTTGAGATCGTCAGCCAGCGCGTCGTTAACGATAAAACCATCGCGTGCCAGTTTAAACGCGGGCT  >  NC_000913/3585824‑3586312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑