Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	452,197	G→A	A450V (GCG→GTG)	ampG ←	muropeptide:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	452,197	0	G	A	97.4%	116.0 / NA	38	A450V (GCG→GTG)	ampG	muropeptide:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (21/16);  minor base C (0/1);  total (21/17)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAAC  >  NC_000913/451953‑452420                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  cAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTcc                                                                                                                                                                                                                              >  1:76569/1‑250 (MQ=255)          ccAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAAt                                                                                                                                                                                                                     <  1:197299/250‑1 (MQ=255)                   aaGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGAGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAAcc                                                                                                                                                                                                           >  2:298646/1‑251 (MQ=255)                                  aCAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGc                                                                                                                                                                                            >  1:295292/1‑251 (MQ=255)                                   cAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCt                                                                                                                                                                                           >  1:224494/1‑251 (MQ=255)                                             aaaGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑Acgcccgcc                                                                                                                                                                                <  2:67170/250‑1 (MQ=255)                                                  gATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCgccgcc                                                                                                                                                                             >  1:41999/1‑249 (MQ=255)                                                                        taataaTTACGGCGAGAGATTCCTACAGCTAAATAATATTTACAGATTACGTCAGCTGCGTTTTTCGTAGCGCCAGATAATCCAGCAAAACACCAAGCACGACGAAAGAAAGGGCGACTAAAACCCCGCCTTCCAACAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAAACACCCGGCCAACAGACTGCAC‑CCCGCCGCCAGTGTCCACATGGCAAAGGCATa                                                                                                                                                      >  2:254611/1‑250 (MQ=255)                                                                                        tGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCgg                                                                                                                                     >  2:109243/1‑251 (MQ=255)                                                                                            tactacAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑g                                                                                                                                >  1:23211/1‑251 (MQ=255)                                                                                                   gCTAAATAATATTTACAGATTACGTCAGATGCGTGTATCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGGCGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTCC‑GGGAGATa                                                                                                                         <  2:321436/251‑1 (MQ=255)                                                                                                      aaaTAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATaa                                                                                                                        <  2:158805/249‑1 (MQ=255)                                                                                                          aataTTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCCGATAATCCCGCAAACCACCCAGCACGACGCCAGAAAAGGCGACTAAAACCCCGACTTCCAGAAGAGCAGGCAGGAAAGCGAAGTGCGTCAAATCCAGAATGTCCATCGTCAATAGCAGTACCCACACGGCCCACAGGCTG‑ACGCCCGACTCCAGTGTCCACATGGCACAGGCATAACATGCCGGATATGCGGTAC‑GGGAGATATAGTTGt                                                                                                                  >  1:440107/1‑251 (MQ=255)                                                                                                             atTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGCGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAGAGTTGTCa                                                                                                                <  1:298646/250‑1 (MQ=255)                                                                                                                tACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGTCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCAttt                                                                                                             <  1:278538/250‑1 (MQ=255)                                                                                                                tACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATACGCGGTAC‑GGGAGATAAAGTTGTCAttt                                                                                                             >  1:209292/1‑250 (MQ=255)                                                                                                                           tCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACAAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTatat                                                                                                 >  2:122244/1‑251 (MQ=255)                                                                                                                               aTGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCa                                                                                              >  2:52330/1‑250 (MQ=255)                                                                                                                                                    aTAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGGCATTTTCTCGTGTATATTCAAGCGTCTGGCGGCAAACcagca                                                                        <  2:317278/251‑1 (MQ=255)                                                                                                                                                           aGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCATCAAAAt                                                                 <  1:125069/251‑1 (MQ=255)                                                                                                                                                             cAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATaa                                                               <  1:52330/251‑1 (MQ=255)                                                                                                                                                             cAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATaa                                                               <  1:261249/251‑1 (MQ=255)                                                                                                                                                             aaaaCCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATaa                                                               <  2:23211/250‑1 (MQ=255)                                                                                                                                                                     caAGCACGACGCGAGAAAGGGCGGCTAAACCCACGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGCGCGACAAATCCACCACGTCCTTCGGCGATAGCAGTAACCACACGGCCAAAAGGCAG‑ACGCCCGCCGCGAGGGTCCACAGCGCAAAAGCATAACCTGCCGGATATGCAGTACAGGGA‑AGAAAGTAGTCATTTACTCGTGTATATTAACGCGTCTGGCGGCCACCCAGAGGCAAAATACGCCGTGGt                                                       >  2:81140/1‑251 (MQ=255)                                                                                                                                                                         cACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGCGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACgg                                                   >  1:71834/1‑251 (MQ=255)                                                                                                                                                                             acgCCAGAAAGGGCGACTAAAACCCCGACTCCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATGCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAgc                                               <  2:71834/251‑1 (MQ=255)                                                                                                                                                                              cgcaataaaGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCCCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAgcg                                              <  2:296191/245‑1 (MQ=255)                                                                                                                                                                                     aaGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCa                                                                                <  2:105760/210‑1 (MQ=255)                                                                                                                                                                                     aaGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCa                                                                                >  1:105760/1‑210 (MQ=255)                                                                                                                                                                                         gCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATACGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGaa                                   <  2:209292/251‑1 (MQ=255)                                                                                                                                                                                              tAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAat                                  >  1:242622/1‑247 (MQ=255)                                                                                                                                                                                               aaaaCCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAatagat                              >  1:299776/1‑250 (MQ=255)                                                                                                                                                                                                aaaCCCCGACTTCCAGCAGCTAAGTCAGACAAAAGAAGCGCGTCAACTCCAGCACGGCCATCGTCAATAGCAGTAACCACACGGCCAACAGCATG‑ACGCCCGCCGCCCTTGTCCACATGACAACGGCATAACCTGCCGGATATGCGGTAC‑GGGACATACAGTTGTCATTTACTCGTGTCTATTCCAGCGTCTGACGGCACACCAGCAGCACAATAAGCCCTGGTActgccgcgtcaccggagaatccat                              >  2:190667/1‑226 (MQ=255)                                                                                                                                                                                                    cccGACTTCCAGAAGAGCAGGCAGGAAAGAGAAGAGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGCAACAACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGAAAAAGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTAATTTACTCGTGTATATTCCAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCTACGGAGCATAGATAGAATg                        >  2:88576/1‑251 (MQ=255)                                                                                                                                                                                                            ccAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAACCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCTGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAg                <  2:41999/251‑1 (MQ=255)                                                                                                                                                                                                                cagagcagGCAGGAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCgt            >  1:119820/1‑251 (MQ=255)                                                                                                                                                                                                                      gggcaggAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCtt      <  2:295292/250‑1 (MQ=255)                                                                                                                                                                                                                           ggAAAGAGAAGTGCGTCAAATCCAGCACGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATACAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCACACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTcaac  >  1:272581/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  CAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCGCGTCCATCGTCAATAGCAGTAACCACACGGCCAACAGGCTG‑ACGCCCGCCGCCAGTGTCCACATGGCAAAGGCATAACCTGCCGGATATGCGGTAC‑GGGAGATAAAGTTGTCATTTACTCGTGTATATTCAAGCGTCTGGCGGCAAACCAGCAGCAAAATAAGCCCTGGTACGGCAGCGGCGACGGAGAATAGATAGAATGTCGACCAGCCGTGTGCTTCAAC  >  NC_000913/451953‑452420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑