Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	646,502	G→A	P27S (CCC→TCC)	citT ←	citrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	646,502	0	G	A	100.0%	63.6 / NA	21	P27S (CCC→TCC)	citT	citrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/11);  total (10/11)

CCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATC  >  NC_000913/646255‑646732                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       ccAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGAc                                                                                                                                                                                                                                     >  1:89350/1‑251 (MQ=255)             cATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGGATAAGaa                                                                                                                                                                                                                          >  1:24326/1‑250 (MQ=255)              aTACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCGGCGGCGGCATACCGTCGGAGACAGGGATAAGAAAc                                                                                                                                                                                                                        <  2:24326/251‑1 (MQ=255)                     ggtgCTGGAAAATCCAGCCAGGCCCCATTTCAGCGCCTGTGTTTGCGCATTAGACGCTGGGTCAGCTAATTCTTTTGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAATACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCTCAAACACAGCGAAGTAATGCCATGCCTGCGGCCGCATACCGTCGGAGACAGGGATAAGGAACATGaca                                                                                                                                                                                                                  <  2:63646/250‑1 (MQ=255)                      gtgCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGGATAAGAAACATGACAcc                                                                                                                                                                                                                <  2:237795/251‑1 (MQ=255)                                            cccATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGGCTAAGAAACATGACACCCATCACCACCAGTGGTGCCaa                                                                                                                                                                                           >  2:78488/1‑250 (MQ=255)                                                    aGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGATAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGTATAAGAAACCTGACACCCATCACCACCAGTGGGGCCAATAGTTTCCa                                                                                                                                                                                  <  1:156212/251‑1 (MQ=255)                                                       gcCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCatat                                                                                                                                                                               <  2:172297/251‑1 (MQ=255)                                                           gTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGGATAATAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCATAtatt                                                                                                                                                                            <  2:39938/250‑1 (MQ=255)                                                                                            cTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGACATGCCTGCGGCGGCACACCGTCGGAGACAGGGATAAGAAACATGACACCCATCCCCACCAGAGGGGCCAATAGTTTCCATAAATTATCTTTTGCTAAAGACATACGGGTTCTCCGaaa                                                                                                                                           >  2:280022/1‑250 (MQ=255)                                                                                                        agagCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGGATAAGAAACATGACTCCCATCACCACCAGTGGGGCCAATAGTGTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCa                                                                                                                              <  1:260086/251‑1 (MQ=255)                                                                                                            cAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAAtt                                                                                                                          <  2:89350/251‑1 (MQ=255)                                                                                                               gTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTAt                                                                                                                       >  1:6883/1‑251 (MQ=255)                                                                                                                  aTTACTGCCAATAACGCAAATAGTAACCGCCATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCa                                                                                                                     >  2:224765/1‑250 (MQ=255)                                                                                                                           aaTAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTaa                                                                                                            >  1:30715/1‑250 (MQ=255)                                                                                                                                          aaCCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAATCCAGCGAAGTAATGCCATGCCTGCGGCGGCACGCCGTCGGAGACAGGGATAAGAAACATGACACCCATCACCTCCAGTGGGGCCAATAGTTACCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCTAAATTTCTCAAGTGCTTAAATAATTAAATCtttg                                                                                             <  2:174308/250‑4 (MQ=255)                                                                                                                                          aaCCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGc                                                                                            >  2:375241/1‑251 (MQ=255)                                                                                                                                                    aaaCTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAAcc                                                                                    >  2:71369/1‑249 (MQ=255)                                                                                                                                                          aTCGCTGTTGCCGGAATTGGATCGAGGATCATGCCGACAATCATTGCCACAAACACCGCGAAGTAATGCCATGCCTGCGGCGGCATCCCGTCGGAGACAGGGATAAGAAACATGACACCCCTCACCACCCGTGGGGCCCATAGTTTCCATATATTATCTTTTGCTAACGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCACGTGCTTAAATAAATAACTCTGTGCTAAAAAACAGGTAAgg                                                                            >  2:432200/1‑251 (MQ=255)                                                                                                                                                                    ccGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGAGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCGTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGt                                                                  <  2:105613/251‑1 (MQ=255)                                                                                                                                                                                                                                      gcggcgGCATACCGTCGGAGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATc  <  1:378408/249‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                       CCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAATTCTTTGGCATCAAAGAGCAGGTAATTACTGCCAATAACGCAAATAGTAACCGCAATAAAACTGATCGCTGTTGCCGGAATTGGCTCGAGGATCATGCCGACAATCATTGCCACAAACACAGCGAAGTAATGCCATGCCTGCGGCGGCATACCGTCGGGGACAGGGATAAGAAACATGACACCCATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATC  >  NC_000913/646255‑646732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑