Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	715,516	G→A	W35* (TGG→TGA)	ybfP →	lipoprotein YbfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	715,516	0	G	A	100.0%	81.3 / NA	25	W35* (TGG→TGA)	ybfP	lipoprotein YbfP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/10);  total (15/10)

GCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGT‑AGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTC‑AACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTT  >  NC_000913/715287‑715739                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                gccaaCGAGGAGGGTGAAAAATATTGAATTTAAGAGATTTTGA‑AGGCTGGAGAAGGAGTTAACGCGGCATCCGGAAAAAACAAAGAACACTTTGTC‑AACAAACGGAGTAGCTCAAGGAAATCCCAAGGAAGACCAAGAGGAGTCTGGTCGTAAAAGTGACACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATaaa                                                                                                                                                                                                            <  2:311584/247‑1 (MQ=255) gCCTGCGAGGATGGTGAAATATATAGAATTTAAGCTATTTTGT‑AGGCCGGAAAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTC‑AACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGCCCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATaaa                                                                                                                                                                                                            <  1:72535/251‑1 (MQ=255)        aGGATGGTGCAATATATTGAATTTAAGCGATTTTGT‑AGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTC‑AACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCag                                                                                                                                                                                                       >  1:315710/1‑249 (MQ=255)               ggaaaTAAATTTAATTTAAGCGATTTTGT‑AGGCCGGATAAGGCTTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTC‑AACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAAc                                                                                                                                                                                              <  1:99150/248‑1 (MQ=255)                gaaaTATATTGAATTTAAGCGATTTTGT‑AGGCCGGAAAAGGCGTTCACGCCGCATCCGGAAAAAACAACGAACACTTTGTC‑AACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACg                                                                                                                                                                                             <  1:122191/249‑1 (MQ=255)                        atGAATTTTAGGGATTTTTTCAGGCAGGATAAGGCTTTCACCCCGCTTCCGGAAAAACCAACAAACA‑TTTGTCCAACAAACTGATTAGCTCAAGAAAATCCCAATGAGTACCAAATGGAGTCGGGTCTTCATATTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTATCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGc                                                                                                                                                                                       <  2:295130/249‑1 (MQ=255)                                 aagagatttttaaGGCCGTATAGGGCTTTCACGCGGCATCCGGCAAAAACAACGAACACTTTGTC‑AACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCTTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTa                                                                                                                                                                              <  1:214961/238‑1 (MQ=255)                                    gATTTTGT‑AGGCCGGAAAAGGCGTTCACGCCGCATACGGCAGAAACAACGAACACTTTGTC‑AACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAatat                                                                                                                                                                         >  1:155591/1‑251 (MQ=255)                                             agcccgaaTAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTC‑AACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCCCTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTTCACCtt                                                                                                                                                                 <  2:101008/244‑1 (MQ=255)                                                     tAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTC‑AACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGgtgt                                                                                                                                                         >  1:217292/1‑251 (MQ=255)                                                                     cATCCGGCAAAAACAACGGACACTTTGTC‑AACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACAAACAGGAGTCTGGACGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACACATTGCCgtg                                                                                                                                         >  2:375949/1‑251 (MQ=255)                                                                                                                                                    ggTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCataat                                                            >  1:181640/1‑250 (MQ=255)                                                                                                                                                                        ttACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAACGAACTTCCTTATCTTCATAATATGTCAGCACAAGAAACGGTc                                       >  1:34429/1‑251 (MQ=255)                                                                                                                                                                              ccACATTCCTGCTGACTGCATGCGCCCACCCTGAACAATAATCATTAGCGGGCGACTGATTACTGACCCAAAAAGAAAAAACCAGAGGATAAACGGGTAGCATTGCGGTAAATATTGCACTTTTCCGGTGTAAGAAAAATTGCCGTGGTGATAACTTCCCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTa                                 <  1:426279/251‑1 (MQ=255)                                                                                                                                                                                acaTTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTaa                                >  1:396458/1‑250 (MQ=255)                                                                                                                                                                                  aTTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATc                             >  1:334046/1‑251 (MQ=255)                                                                                                                                                                                       tgctgACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAACTATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCtt                                                                  >  1:285702/1‑209 (MQ=255)                                                                                                                                                                                         ctgACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGt                                    >  2:315089/1‑237 (MQ=255)                                                                                                                                                                                         cggACTGCATGCGCCAAACCTAAACAATCATCATAAGCTGGCAACTGATTACTGCCCCCAAAAAAAAAACCCAGAGGATAAACGGGTAGCATGGCGTTAAATATTGCCCCTTCCCGTTGAAAGACAAATGGCGGTGGTGATAACTTCCCTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGt                                    <  1:315089/235‑1 (MQ=255)                                                                                                                                                                                           gACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCg                                      >  1:117632/1‑233 (MQ=255)                                                                                                                                                                                               gCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGat                                                                                                                        >  2:110232/1‑147 (MQ=255)                                                                                                                                                                                               gCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGat                                                                                                                        <  1:110232/147‑1 (MQ=255)                                                                                                                                                                                                cATGCGCTCAACCTGAACAATCATCATTAGCTGGCGACTGAATCCTGACCCCTAAACATAAAACCAGGGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAAGTGCCGTGGTCATAACTTAGCTGATAATACCCGCCGCTAGCAGATGTCGGGCGGGAACGAAAAAGATCTTTCTTATCTTCATAATATGTCAGCCCAAGTAAAGGTCGGTTTCAATCTCGGTTGGCAATGc               >  1:246983/1‑251 (MQ=255)                                                                                                                                                                                                         aaCCTGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTAACGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACATTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTAAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTc       >  1:266858/1‑250 (MQ=255)                                                                                                                                                                                                             tGAACAATCATCATTAGCTGGCGACTGATTACTGACCCCTCAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGACGGTTTAAATCCCGGTTGGCAATGCTACACCTCAtttt  >  2:392494/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                GCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGT‑AGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTC‑AACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTT  >  NC_000913/715287‑715739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑