Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,494,388	Δ1 bp	coding (684/924 nt)	ydcI ←	DNA‑binding transcriptional dual regulator YdcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,494,388	0	C	.	98.3%	267.0 / ‑3.2	59	coding (684/924 nt)	ydcI	DNA‑binding transcriptional dual regulator YdcI
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base . (26/32);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

TCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAA  >  NC_000913/1494148‑1494637                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                          tCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAAtttt                                                                                                                                                                                                                                                >  1:395151/1‑251 (MQ=255)   aGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTAc                                                                                                                                                                                                                                              >  2:150598/1‑251 (MQ=255)           aTTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTgg                                                                                                                                                                                                                                      <  1:151147/251‑1 (MQ=255)              gATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGGCAGCGTTTCGATACA‑GCCGAAGGAATTTTACAGCCCTGGctc                                                                                                                                                                                                                                   <  1:136547/251‑1 (MQ=255)                    cGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGAGACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACt                                                                                                                                                                                                                             <  1:105515/251‑1 (MQ=255)                        tatcgctAATCATCAACTGGCAACCCGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCAGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGACAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGCCGAGATAGCGAAGCAGACATCGTTTCGATACAACA‑GAAGGACTTTTACAGCCCTAGCTCTGCACTAAt                                                                                                                                                                                                                          >  2:34028/7‑250 (MQ=255)                             ttAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGCCAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCt                                                                                                                                                                                                                    >  1:387839/1‑251 (MQ=255)                                  catcaACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTTGATACAACC‑GAAGGAATTTTACAGCCCTGGCACTGCACTAATGCATCTGAATg                                                                                                                                                                                                               >  2:334737/1‑251 (MQ=255)                                       aCTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCAAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGAAAGCATTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCtggc                                                                                                                                                                                                          >  1:179142/1‑251 (MQ=255)                                          ggCAACCAGACGAGAACGTCGCATCTCCGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTCATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGACGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCCGCACTAATGCCTCTGAATGCtggcgcg                                                                                                                                                                                                       >  2:280712/1‑249 (MQ=255)                                            cAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCtggcgtggc                                                                                                                                                                                                     <  1:280652/251‑1 (MQ=255)                                                cAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTa                                                                                                                                                                                                                                               >  2:294141/1‑205 (MQ=255)                                                cAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTa                                                                                                                                                                                                                                               <  1:294141/205‑1 (MQ=255)                                                      aaaaCGTCGCATCTACGCGGGTCAGTATATCAATCGGTTCGCCTGCCCCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCta                                                                                                                                                                                           <  2:327559/249‑2 (MQ=255)                                                                aTCTACGCGGGTCAGTATTCCAATCGGTTCGACTGCAACATGTACCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTAAAGCGCCAGAAGGGACAAACAACACGTAATCGTATTCAACGGAAAGTTGACGAGAAAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAAATTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGCGGCGCAGTGCCTTCTGGAGATAc                                                                                                                                                                                 >  1:148887/1‑251 (MQ=255)                                                                    aCGCGGGTCAGTATTCCAATCGGTGCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACaa                                                                                                                                                                               <  1:191540/249‑1 (MQ=255)                                                                         ggTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCgg                                                                                                                                                                        <  1:355533/251‑1 (MQ=255)                                                                         ggTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCgg                                                                                                                                                                        >  2:229556/1‑251 (MQ=255)                                                                         ggTCAGGAGTCCAATCGTGTCGCCTGCACCATGTCCCGGAACACGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGCCAAACCACACGTATTCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCTCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCgg                                                                                                                                                                        <  2:384552/251‑1 (MQ=255)                                                                              gTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCAtt                                                                                                                                                                   <  1:320740/251‑1 (MQ=255)                                                                                 ttCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAAtttt                                                                                                                                                                                                                                                <  2:362847/171‑1 (MQ=255)                                                                                 ttCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAAtttt                                                                                                                                                                                                                                                >  1:362847/1‑171 (MQ=255)                                                                                     aaTCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGAGGCAGGTCGTCTTTTACAGAGCCAGAAAGGACAAACCACACGTAGGCGTATTCAACCGTAAGATGACGAGATAACACAGCAGACAGAGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCACTGCACTAATGCATCTGAATGCTGGCGTTGCGCAGTGCCTTCTGGTAATACAACCACCCGCCAGTcccgccc                                                                                                                                                            >  1:51837/1‑246 (MQ=255)                                                                                                cTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGCCAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTAAAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCg                                                                                                                                                 >  1:288031/1‑251 (MQ=255)                                                                                                  gCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGtt                                                                                                                                               <  2:106834/251‑1 (MQ=255)                                                                                                      cATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGt                                                                                                                                           <  1:229556/251‑1 (MQ=255)                                                                                                                  caggcaggGCCCCCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGATGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGt                                                                                                                                >  2:250211/1‑250 (MQ=255)                                                                                                                  caggcaggGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGaa                                                                                                                                                                                                                 >  2:387468/1‑169 (MQ=255)                                                                                                                  caggcaggGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGaa                                                                                                                                                                                                                 <  1:387468/169‑1 (MQ=255)                                                                                                                                        aTGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAAc                                                                                                         >  1:316266/1‑251 (MQ=255)                                                                                                                                         tGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAAcc                                                                                                        >  2:72818/1‑251 (MQ=255)                                                                                                                                              cAGGTCGTCTATTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GACGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTCAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCTGACAACCAGCtt                                                                                                   <  2:58931/251‑1 (MQ=255)                                                                                                                                                  tcgtcTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGt                                                                                                                                                                                                <  1:180049/154‑1 (MQ=255)                                                                                                                                                   cgtcTTTTACAGCGCCAGAAGGGACAAACCACACGTAAGCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAAc                                                                                               <  1:266689/250‑1 (MQ=255)                                                                                                                                                     tcTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGAt                                                                                            >  1:425139/1‑251 (MQ=255)                                                                                                                                                         ttACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCa                                                                                         >  1:149154/1‑250 (MQ=255)                                                                                                                                                           aCAGCGCCAGCATGTACAAACCACACTTAATCGTATTCAACCGTAAGTTGAAGAGAGAGCGAAGCAGACAGCGTTTCGATACTACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCTCCCGGCTTAGCGTTACGTTCTCTTGAAGTAGCGGGTGATTAGGGCGGACAACCCGCTTCAACGATTCAAGa                                                                                      <  2:403179/251‑1 (MQ=255)                                                                                                                                                           aCAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGa                                                                                      <  2:402869/251‑1 (MQ=255)                                                                                                                                                            cAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCTTTTCGATACAACC‑GAAGGAAtttt                                                                                                                                                                                                                                                <  2:28042/96‑1 (MQ=255)                                                                                                                                                            cAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAAtttt                                                                                                                                                                                                                                                >  1:28042/1‑96 (MQ=255)                                                                                                                                                               cgcCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGCCGAGATAGCGCCGCCGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTACTGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAAc                                                                                   >  1:67539/1‑250 (MQ=255)                                                                                                                                                                    aaaNGGACAACCCACACGTAATCGTATTCAAACGTAAGTAGACGAGATAGCGTAGCAGACAGCGTATCGATTCAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCTCTAATGCATCTCAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAATAAAcagcatt                                                                             <  2:405488/250‑3 (MQ=255)                                                                                                                                                                      aGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTc                                                                           <  2:24950/251‑1 (MQ=255)                                                                                                                                                                               ccACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTa                                                                    >  1:268536/1‑249 (MQ=255)                                                                                                                                                                               ccACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAg                                                                  <  2:268536/251‑1 (MQ=255)                                                                                                                                                                                cacaCGTAATCGTATTCAACCGTAAGGTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCGAGAAACAGGAGTTCGTAATTAAGc                                                                 <  2:449628/251‑1 (MQ=255)                                                                                                                                                                                 acacGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCTGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGc                                                                 <  1:152375/250‑1 (MQ=255)                                                                                                                                                                                 acacGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCTGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGc                                                                 >  2:152375/1‑250 (MQ=255)                                                                                                                                                                                 acacGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTGCGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGcc                                                                <  2:114078/251‑1 (MQ=255)                                                                                                                                                                                     gTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGt                                                            <  1:150598/251‑1 (MQ=255)                                                                                                                                                                                       aaTCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGTGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGtc                                                           >  1:142588/1‑250 (MQ=255)                                                                                                                                                                                           gTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAg                                                      <  2:78292/251‑1 (MQ=255)                                                                                                                                                                                                     gTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCt                                            <  1:213765/251‑1 (MQ=255)                                                                                                                                                                                                                       gAAGCAGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCg                          >  2:357039/1‑251 (MQ=255)                                                                                                                                                                                                                            aGACAGCGTTTCGATACAACC‑GCAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGATTGCTGGCGTCGCGCAGTGTCGTCTGGTGATACAACGACCGGCCAGTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACACCCAGCTTCACCGAGTCAAGAAACAGCAGTTCGGAATTAAGCCCGGTCATCAGTTCAGGATCTGACAACCGGCCAAGGACGatatt                     <  2:322663/251‑2 (MQ=255)                                                                                                                                                                                                                            aGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGTCAACCATCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATc                     <  2:387839/251‑1 (MQ=255)                                                                                                                                                                                                                            aGACAGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATc                     <  2:316266/251‑1 (MQ=255)                                                                                                                                                                                                                                aGCGTTTCGATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAACCAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTTACATCCGACCAATGCCGATATCGAtt                 <  1:113430/251‑1 (MQ=255)                                                                                                                                                                                                                                        gATACAACC‑GAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGt         >  1:400506/1‑251 (MQ=255)                                                                                                                                                                                                                                                ccGAAGGAATGTTACAGCCCAGGATCTGCACTAATGCATCTAAATGCTTGCGTTGTGCAGGGCCTTCTGGAGATACCACTACCGGTCATTCCAGCACCCGGCTTATCGTTACGTTCTACTAAAGTAGCGGGTGATTATTGCGGACACCCAGCGTCAATGAGTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTAATCACTTCAGGATCCAACATCCGACCAAAGCCGATATCTATTTCCCCTATTTTTaaa  <  1:166459/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                          TCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCATCTACGCGGGTCAGTATTCCAATCGGTTCGCCTGCACCATGTCCCGGAACAGGCAGGGCCACCAGCGTGGCATGACGCAGGTCGTCTTTTACAGCGCCAGAAGGGACAAACCACACGTAATCGTATTCAACCGTAAGTTGACGAGATAGCGAAGCAGACAGCGTTTCGATACAACCCGAAGGAATTTTACAGCCCTGGCTCTGCACTAATGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTTTAAA  >  NC_000913/1494148‑1494637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑