Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	910,295	C→T	G252E (GGG→GAG)	poxB ←	pyruvate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	910,295	0	C	T	100.0%	143.2 / NA	42	G252E (GGG→GAG)	poxB	pyruvate oxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/20);  total (22/20)

AAACTTGCGATCGGCTTTTTCTTCCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGC  >  NC_000913/910057‑910536                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  aaaCTTGCGATCGGCTTTTTCTTCCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGTCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCaa                                                                                                                                                                                                                                       <  1:62196/251‑1 (MQ=255)       gCGATCGGCTTTTTCTTCCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAAcatcat                                                                                                                                                                                                                                 <  1:395252/251‑1 (MQ=255)         gATCGGCTTTTTCTTCCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATAc                                                                                                                                                                                                                               >  1:40763/1‑251 (MQ=255)                        ccaccaATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTc                                                                                                                                                                                                                 <  2:356131/250‑1 (MQ=255)                            caATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACAt                                                                                                                                                                                                            <  1:263603/251‑1 (MQ=255)                                ggAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGtt                                                                                                                                                                                                         <  1:37951/250‑1 (MQ=255)                                  aaGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCtt                                                                                                                                                                                                      >  1:104021/1‑251 (MQ=255)                                           aCGCAGATTCGACTTGAGATCGCCTACCAGTCCCATATCTACCTTGCTGGGAGCTGCGATGCTGGCTGGGTTGATATCAGTCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTATGGAAATTGCGTGCCTAGTATCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGTCGAGAAGCCGATTAACTCGGATATGCCAACATCATACGGATTATCGTACTCGACATGTTCTGTACCGCGCa                                                                                                                                                                                             <  2:176163/251‑1 (MQ=255)                                                gagTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGc                                                                                                                                                                                         >  2:285688/1‑250 (MQ=255)                                                   tCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATg                                                                                                                                                                                      >  1:107958/1‑250 (MQ=255)                                                    cGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATTATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAACCCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCATGGCATGa                                                                                                                                                                                     <  1:43979/250‑1 (MQ=255)                                                     gACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATAACTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGGGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACTGATTATCGTAGTCTACATGTTCTTTACCGCGCAGGGCATGAAc                                                                                                                                                                                   <  1:256179/251‑1 (MQ=255)                                                      aCTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAAc                                                                                                                                                                                   >  1:429689/1‑250 (MQ=255)                                                      aCTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACa                                                                                                                                                                                  <  1:418815/251‑1 (MQ=255)                                                                                       tGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCaac                                                                                                                                                  <  1:298428/250‑1 (MQ=255)                                                                                                       gctggctgGGTGGATAGCAATCTGAATTATTGTGGCATCGGTCGGGTAGAAGGTGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGTTCATTCCAACATCATACGGAGTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGATCAAGATGCGCTTTAATTTTCCCGGCAAATTCAACTAATTCTGTATGCGcct                                                                                                                                 <  2:52047/251‑2 (MQ=255)                                                                                                         tggctggGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGccccc                                                                                                                               >  1:415055/1‑251 (MQ=255)                                                                                                              gggTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAAGTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCcgcg                                                                                                                            <  1:382692/249‑1 (MQ=255)                                                                                                               ggTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCgcgc                                                                                                                           >  1:270844/1‑249 (MQ=255)                                                                                                                                aTGATTTTGGCATCGGTCGGGTAGAAGGCGCGGAAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATc                                                                                                          >  1:172088/1‑249 (MQ=255)                                                                                                                                  gATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAggg                                                                                                      <  1:85494/251‑1 (MQ=255)                                                                                                                                          cATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTg                                                                                              >  2:392303/1‑251 (MQ=255)                                                                                                                                           aTCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGc                                                                                             <  2:40763/251‑1 (MQ=255)                                                                                                                                               gtcggGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTgg                                                                                          >  2:92568/1‑250 (MQ=255)                                                                                                                                               gtcggGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTgg                                                                                          >  1:20205/1‑250 (MQ=255)                                                                                                                                                    gTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAAc                                                                                    <  1:122914/251‑1 (MQ=255)                                                                                                                                                              cgGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTg                                                                          >  1:315513/1‑251 (MQ=255)                                                                                                                                                               aggaGGGAAATTGCGTGCGGAGTAGCACTAACGTTTAGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGACGCGTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTgc                                                                         >  2:175168/4‑251 (MQ=255)                                                                                                                                                                  aGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACAGCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGcc                                                                      <  2:182268/251‑1 (MQ=255)                                                                                                                                                                    ggAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAg                                                                    <  1:20125/251‑1 (MQ=255)                                                                                                                                                                        aTTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGACGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGAAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCACGCGCAGCCGCTGCCACACATCAGGGCGAGATTGCTGGCATAACGCAGCAGTTGCGCCAGTTTg                                                                >  2:205726/1‑251 (MQ=255)                                                                                                                                                                            cGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATGCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTGATGCGCCCCCGCGCAGCCGCTGCCACGCGTCAGGGCGATATTGCGGGAATAACGCAGCAGTTGCGCCAGTTTGCGTa                                                            <  1:409044/251‑1 (MQ=255)                                                                                                                                                                                         aCTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATAGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCttcttcttc                                               >  2:182093/1‑251 (MQ=255)                                                                                                                                                                                          cTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGCTTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTcc                                              >  2:10243/1‑251 (MQ=255)                                                                                                                                                                                                  gcGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCAc                                      <  2:452817/250‑1 (MQ=255)                                                                                                                                                                                                      cGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACt                                  >  1:10627/1‑251 (MQ=255)                                                                                                                                                                                                            tcatGGTATGGAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTtgt                            >  1:254087/1‑251 (MQ=255)                                                                                                                                                                                                                      gAAACCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCGGGCGTCACGACTGGTTGTGGCGCATGAt                  <  1:402288/251‑1 (MQ=255)                                                                                                                                                                                                                         aCCTGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATAcc               >  2:1673/1‑251 (MQ=255)                                                                                                                                                                                                                            tGACGAGAAGCCGATTAACTCGGTCATTCCAACATCATACGGATTATCGCATTCGAAATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCACGGCAATCTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGCCGATATTGATGGAATAACGCAGCTGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAgg            >  1:429532/1‑250 (MQ=255)                                                                                                                                                                                                                                  gaAGCCGATTAACTCGGTCATTCCAACATCATACGGAGTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCNTgg      >  2:411668/1‑251 (MQ=255)                                                                                                                                                                                                                                      ccGATTAACTCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGc  >  2:300521/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  AAACTTGCGATCGGCTTTTTCTTCCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGTATTCGACATGTTCTTTACCGCGCAGGGCATGAACAATAGGCGCTTTAATTTTCCCGGCAAACTCAACTAACTCTTTATGCGCCCCCGCGCAGCCGCTGCCACACATCAGGGCGATATTGCTGGAATAACGCAGCAGTTGCGCCAGTTTGCGTAACTCTTCTTCTTCCGGCGTCACGACTGGTTGTGGCGCATGATACCAGTGCATGGTTGC  >  NC_000913/910057‑910536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑