Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	946,875	G→A	L335L (CTG→CTA)	ycaD →	putative transporter YcaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	946,875	0	G	A	97.6%	134.0 / NA	41	L335L (CTG→CTA)	ycaD	putative transporter YcaD
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (12/28);  total (12/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGGACGTCTGGCGGATAAGTTTGGTCGACTGCTGGTGTTGCGTGTTCAGGTCTTTGTCGTCATTCTCGGCAGTATCGCGATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTGCTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAA  >  NC_000913/946633‑947113                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               aTTGGACGTCTGGCGGATAAGTTTGGTCGACTGCTGGTGTTGCGTGTTCAGGTCTTTGTCGTCATTCTCGGCAGTATCGCGATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGccc                                                                                                                                                                                                                                        <  1:389189/251‑1 (MQ=255)   tGGACGTCTGGCGGATAAGTTTGGTCGACTGCTGGTGTTGCGTGTTCAGGTCTTTGTCGTCATTCTCGGCAGTATCGCGATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGt                                                                                                                                                                                                                                      >  2:336363/1‑251 (MQ=255)     gACGTCTGGCGGATAAGTTTGGTCGACTGCTGGTGTTGCGTGTTCAGGTCTTTGTCGTCATTCTCGGCAGTATCGCGATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTc                                                                                                                                                                                                                                     >  1:9767/1‑250 (MQ=255)     gACGTCTGGCGGATAAGTTTGGTCGACTGCTGGTGTTGCGTGTTCAGGTCTTTGTCGTCATTCTCGGCAGTATCGCGATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCa                                                                                                                                                                                                                                    <  2:59047/251‑1 (MQ=255)               ggATAAGTTTGGTCGACTGCTGGTGTTGCGTGTTCAGGTCTTTGTCGTCATTCTCGGCAGTATCGCGATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCt                                                                                                                                                                                                                           >  1:264779/1‑250 (MQ=255)                   aaGTTTGGTCGACTGCTGGTGTTGCGTGTTCAGGTCTTTGTCGTCATTCTCGGCAGTATCGCGATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGc                                                                                                                                                                                                                       <  2:112989/250‑1 (MQ=255)                                 gctgGTGTTGCGTGTTCAGGTCTTTGTCGTCATTCTCGGCAGTAGCGCGATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTtct                                                                                                                                                                                                        <  2:356753/251‑1 (MQ=255)                                    ggTGTTGCGTGTTCAGGTCTTTGCCGTCAGTCTCGGCAGTATCGCGATGCTTAGCCAGGCGGCGATTGCCCCAGCGTTATTGATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCAATGAACCAGGCCTTACTGTTGAGCTATGCCGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCg                                                                                                                                                                                                     <  1:53505/251‑1 (MQ=255)                                            gtgtTCAGGTCTTTGTCGTCATTCTCGGCAGTATCGCGATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTa                                                                                                                                                                                             <  1:235933/251‑1 (MQ=255)                                                          gtcgtcATTCTCGGCAGTATCGCGATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGAt                                                                                                                                                                               <  2:264779/251‑1 (MQ=255)                                                               cATTCTCGGCAGTATCGCGATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGcc                                                                                                                                                                           <  2:75985/250‑1 (MQ=255)                                                                       gCAGTATCGCGATGCGTAGCCTGGCGGCGATGGCCCCAGCGTTATTCATCGTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCg                                                                                                                                                                  <  2:97608/251‑1 (MQ=255)                                                                              cgctaTGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGGTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGttt                                                                                                                                                               <  2:310560/243‑1 (MQ=255)                                                                              cgcgATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCCCGC‑GGCTTTACGCTATATCCGGTGGAGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGCTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGCTAATTTATTGTTTATCATGATCGCCAGCGTATCGttt                                                                                                                                                               >  1:310560/1‑247 (MQ=255)                                                                              cgcgATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATc                                                                                                                                                            <  1:52653/250‑1 (MQ=255)                                                                              caggATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCt                                                                                                                                                           <  2:293571/248‑1 (MQ=255)                                                                                        aGCNAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAAGTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGAt                                                                                                                                                 <  2:405276/251‑1 (MQ=255)                                                                                        aGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGa                                                                                                                                                  <  1:327459/250‑1 (MQ=255)                                                                                           cAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGAtgct                                                                                                                                              >  1:38530/1‑251 (MQ=255)                                                                                                 gcgATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACACTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTgcg                                                                                                                                        >  2:362150/1‑251 (MQ=255)                                                                                                           cAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGt                                                                                                                              >  1:123115/1‑251 (MQ=255)                                                                                                                           cGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCg                                                                                                              >  1:384391/1‑251 (MQ=255)                                                                                                                             gTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGtt                                                                                                            <  1:336363/251‑1 (MQ=255)                                                                                                                                           tACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTaa                                                                                                <  1:288112/250‑1 (MQ=255)                                                                                                                                            aCGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTaaa                                                                                               <  2:22172/250‑1 (MQ=255)                                                                                                                                                     ccGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAg                                                                                     <  1:385685/251‑1 (MQ=255)                                                                                                                                                                   aTGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACtt                                                                       <  1:286626/251‑1 (MQ=255)                                                                                                                                                                     gggCTTGCAAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCAATGCTGTGGGAAGTCTACTTGGCCCGTCAGTTACCGCTATGCTAATGCCGAATTTCTCCGATAATTTATTGTTTATCATGGTCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGTCGAAGCCCGTTGCTCTCGTGTAAATGAATTCAAGCAGAGTGTGAACTTa                                                                      <  2:238001/250‑1 (MQ=255)                                                                                                                                                                       gCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTg                                                                   <  1:79894/251‑1 (MQ=255)                                                                                                                                                                           gCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGttt                                                                <  1:406166/250‑1 (MQ=255)                                                                                                                                                                           gCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTGCTCCGATAATTTATTGTTTATCATGATCGCCAGTGTAGCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAAGCCGTTGCGCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGttt                                                                <  1:254134/250‑1 (MQ=255)                                                                                                                                                                                                          gATGAGCCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTGACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGAc                                <  2:255893/251‑1 (MQ=255)                                                                                                                                                                                                             gAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTg                             >  2:458547/1‑251 (MQ=255)                                                                                                                                                                                                             gAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTg                             <  2:111496/251‑1 (MQ=255)                                                                                                                                                                                                                     ccTTACTGTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTc                     >  1:41835/1‑251 (MQ=255)                                                                                                                                                                                                                            gTTGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAAt                <  1:266322/249‑1 (MQ=255)                                                                                                                                                                                                                             ttGAGCTATACTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAAtttt             >  2:960/1‑251 (MQ=255)                                                                                                                                                                                                                                    ataCTGTTGGAAGTCTGCTTTGCCCGTCACTGACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAATCCCGTGGCTCACGTGTAAATGAATTCAATCAGAGTGTTAACTTACTGTTTCACGCTCTGCTTTTTTGTTTCTTCTATCCGCCTTGCTTTATTCCAACTTTAAttag        <  2:90129/249‑3 (MQ=255)                                                                                                                                                                                                                                      aCTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGttt     <  2:317198/250‑1 (MQ=255)                                                                                                                                                                                                                                       cTGTGGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTa    >  2:377510/1‑250 (MQ=255)                                                                                                                                                                                                                                         gtgGGAAGTCTACTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTaaa  <  1:377510/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               ATTGGACGTCTGGCGGATAAGTTTGGTCGACTGCTGGTGTTGCGTGTTCAGGTCTTTGTCGTCATTCTCGGCAGTATCGCGATGCTTAGCCAGGCGGCGATGGCCCCAGCGTTATTCATCCTCGGTGCC‑GCTGGCTTTACGCTATATCCGGTGGCGATGGCATGGGCTTGCGAGAAAGTTGAACATCATCAACTGGTGGCGATGAACCAGGCCTTACTGTTGAGCTATACTGTGGGAAGTCTGCTTGGCCCGTCATTTACCGCTATGCTAATGCAGAATTTCTCCGATAATTTATTGTTTATCATGATCGCCAGCGTATCGTTTATCTATTTGCTGATGCTGCTGCGCAACGCCGGTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAA  >  NC_000913/946633‑947113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑