Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	446,036	C→T	E211K (GAA→AAA)	yajR ←	putative transport protein YajR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	446,036	0	C	T	100.0%	167.0 / NA	49	E211K (GAA→AAA)	yajR	putative transport protein YajR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/21);  total (28/21)

CGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAAGCACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTT  >  NC_000913/445802‑446273                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              cGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGCCGCCATCAGCAAAATATGAAGACACATAATGCAAAAGATGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACt                                                                                                                                                                                                                                >  2:360387/1‑250 (MQ=255)  gATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTC‑CCAGTGTTCAGCCGCCGGGAACCCTACATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTg                                                                                                                                                                                                                               >  1:415663/1‑250 (MQ=255)                            cTTGCTGCATTTTGCGCTTAACTTCAGCGTAGATAATGAACGGAACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGCCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGCTTCGCCAGCACTTTCCTGAAACTGCCTTTCACCATTCCGGACTc                                                                                                                                                                                                     >  2:34818/1‑250 (MQ=255)                               gCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCTAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACg                                                                                                                                                                                                  >  2:26198/1‑250 (MQ=255)                                        tGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCTAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTAc                                                                                                                                                                                         <  1:26198/250‑1 (MQ=255)                                               aaCTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGc                                                                                                                                                                                 >  2:343054/1‑251 (MQ=255)                                                 cTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCCTCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGCTTCAGCAGCAGCGGTTTCGCCAGCACTTTACAGAAACTGCCTTTCACCATTCCTGACTCACGATTAAGTACGTGAganct                                                                                                                                                                                >  2:405914/1‑246 (MQ=255)                                                     aGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTANTGt                                                                                                                                                                           >  2:414164/1‑251 (MQ=255)                                                        gTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTgg                                                                                                                                                                        >  1:240702/1‑251 (MQ=255)                                                                  aaaGGAACGACCGCGCACAAGGCGATTAGCATTGTCGCCCGATAGACCTTACCA‑TGTCCAGCCGCAGGGTACACTGCATCAGACAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAACACAATATGCAGACACATACTGCCAAAGTTGAGTTTCAGCAGCTGCGGGTTCGCCAGCACTTTACTGAAACTGCCTTCCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAAccc                                                                                                                                                               >  2:424213/1‑250 (MQ=255)                                                                     ggCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAAt                                                                                                                                                           <  2:240702/251‑1 (MQ=255)                                                                      gCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACGCACGATTAAGTACGGGAGTGCTACTGTTGGGCACAACCCAAATg                                                                                                                                                          <  2:440550/251‑1 (MQ=255)                                                                       cACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATgg                                                                                                                                                         >  1:84678/1‑251 (MQ=255)                                                                              gAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTg                                                                                                                                                                                  >  2:454683/1‑219 (MQ=255)                                                                              gAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTg                                                                                                                                                                                  <  1:454683/219‑1 (MQ=255)                                                                              gAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGGCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGc                                                                                                                                                  <  2:359664/251‑1 (MQ=255)                                                                                  cAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATg                                                                                                                                              <  1:425313/251‑1 (MQ=255)                                                                                   aaaGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGc                                                                                                                                             <  2:117811/251‑1 (MQ=255)                                                                                     aGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCCAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCg                                                                                                                                           >  1:343709/1‑251 (MQ=255)                                                                                       gCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGt                                                                                                                                         <  2:235841/251‑1 (MQ=255)                                                                                                                  tt‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACg                                                                                                                                                   <  2:27613/214‑1 (MQ=255)                                                                                                                  tt‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACg                                                                                                                                                   >  1:27613/1‑214 (MQ=255)                                                                                                                  tt‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGa                                                                                                               >  1:345676/1‑250 (MQ=255)                                                                                                                     ccAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCAACCAGaaa                                                                                                             >  1:446671/1‑249 (MQ=255)                                                                                                                             cAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCATGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTg                                                                                                     <  1:262957/250‑1 (MQ=255)                                                                                                                                ccgccgGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGc                                                                                                 >  1:69261/1‑251 (MQ=255)                                                                                                                                 cgccgGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGcc                                                                                                <  2:445620/251‑1 (MQ=255)                                                                                                                                           ccTGCATCAGAAAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACCGCGCGTGCAGCCCAAGATtgtg                                                                                      >  2:40267/1‑251 (MQ=255)                                                                                                                                               cATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTgtgagtg                                                                                  >  1:293240/1‑251 (MQ=255)                                                                                                                                                aTCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTgtgagtg                                                                                  >  1:317915/1‑250 (MQ=255)                                                                                                                                                        gTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCggg                                                                         <  2:317915/251‑1 (MQ=255)                                                                                                                                                          tGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGc                                                                        <  1:97421/250‑1 (MQ=255)                                                                                                                                                                       caacaaACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAAGCACCATCGCa                                                          <  2:293240/251‑1 (MQ=255)                                                                                                                                                                               gTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAAGCACCATCGCAATGGCaa                                                   >  1:101625/1‑250 (MQ=255)                                                                                                                                                                                 cGACATCAGCAAAATATGCAGACACATAATGCCAAAGTGTAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTGACTGAAACTGCCTGTCACCATGCCGGACGCGCTATTAAGGACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAAGAGCGATCATCCAGAACAGCGCGTGCAGGCCAAGTTTGTTAGTGATGAGCGTGCTAAGCTCCATCGTAATGGCAAAg                                                 <  2:428672/250‑1 (MQ=255)                                                                                                                                                                                     aTCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGGGCAGCCCACGTTTGTGAGTGATGATCGGGCCAAGCACCATCGCAATGGCAAAGGTAAt                                            >  1:174923/1‑251 (MQ=255)                                                                                                                                                                                                atGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAAGCACCATCGCAATGGCAAAGGTAATGCCAAAGCTc                                  >  2:107556/1‑250 (MQ=255)                                                                                                                                                                                                  gCAGACACATAATTCCCAAGTTTAGTTTCAGCAGCCGCGGTTTCGGCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATTATCGGGCCAAGCACCATCGCAATGGCAAAGGTAATGCCAAAGCTCAc                                <  2:262431/250‑1 (MQ=255)                                                                                                                                                                                                   cAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAAGCACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGc                              >  1:206118/1‑251 (MQ=255)                                                                                                                                                                                                    atanacATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAATTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCGTATTACCATCGCAATTTCAAAGGTAATTCCAAAGCTCTCGcc                             <  2:415663/249‑1 (MQ=255)                                                                                                                                                                                                     gACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAAGCACCATCGCAATGGCAAAGGAAATGCCAACGCTCACGccc                            >  2:138006/1‑250 (MQ=255)                                                                                                                                                                                                             aTGCCAAAGTTGAGTTTCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAATCACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGc                    <  1:435548/251‑1 (MQ=255)                                                                                                                                                                                                                           tttCAGCAGACGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCAGGACTCACGATTAAGTACGAGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAAGCACCATAGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGATTTGGTGc      >  1:417550/1‑251 (MQ=255)                                                                                                                                                                                                                            ttCAGCAGCCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAAGCACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCg     >  1:42031/1‑251 (MQ=255)                                                                                                                                                                                                                               agcagcCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCCCCATTCCGGTCTGACGATTAAGTACGTGAGTGCAATTGTGGGGCACAACCCAAAGGGTCAACGGAATGCCGGTCGTTGCCAGAATAGCGATCTTCCAGAACAGGGCGTCCACCCCAAGTTTGTGAGTGAGGATCGGGCCAATCCCCAGCGCAAGGGCAATGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGtt  <  1:47410/251‑1 (MQ=255)                                                                                                                                                                                                                               agcagcCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAAGCACCATCGCAATGGCAAAGGTAATGCCa                                        <  2:311263/213‑1 (MQ=255)                                                                                                                                                                                                                               agcagcCGCGGTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAAGCACCATCGCAATGGCAAAGGTAATGCCa                                        >  1:311263/1‑213 (MQ=255)                                                                                                                                                                                                                                        ggTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAAGCACCATCGCAATGGCAAAGGTAATGCCAAAGCTCAc                                <  2:19774/212‑1 (MQ=255)                                                                                                                                                                                                                                        ggTTTCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAAGCACCATCGCAATGGCAAAGGTAATGCCAAAGCTCAc                                >  1:19774/1‑212 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              CGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTT‑CCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCAGTTGTCCGGGCAGGGCAACAAACGTCGACATCAGCAAAATATGCAGACACATAATGCCAAAGTTGAGTTTCAGCAGCCGCGGTTCCGCCAGCACTTTACTGAAACTGCCTTTCACCATTCCGGACTCACGATTAAGTACGTGAGTGCTACTGTTGGGCACAACCCAAATGGTCAACGCAATGCCGGTCGTTGCCAGAATAGCGATCATCCAGAACAGCGCGTGCAGCCCAAGTTTGTGAGTGATGATCGGGCCAAGCACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTT  >  NC_000913/445802‑446273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑