Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,641,902 G→A M347I (ATG→ATA)  dtpB → dipeptide/tripeptide:H(+) symporter DtpB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,641,9020GA97.6% 143.1 / ‑6.5 42M347I (ATG→ATA) dtpBdipeptide/tripeptide:H(+) symporter DtpB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (20/21);  minor base C (1/0);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GTCGCCTTTGTCCTGATGCTCGAAGCGGTGGTGTTTTACATTCTCTACGCCCAGATGCCAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATGAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCGATGATTGCTGCCCTGGTGCCGCAGCATTTGATGGGCTTTATTCTCGGGATGTGGTTCCTGACGCAGGCTGCCGCGT  >  NC_000913/3641669‑3642143
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 
gTCGCCTTGGGCCTGATGCTCGAAGCTGTGGTGTTTTACATTCTCTACGCCCAGATGCCAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATCATCCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGTGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCAt                                                                                                                                                                                                                                  <  2:4509/251‑1 (MQ=255)
 tCGCCTTTGTCCTGATGCTCGAAGCGGTGGTGTTTTAAATTATCTACGCCCAGATGCCAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGAGCTCTCGATGCCGATACAATTTACTCTCGGCATg                                                                                                                                                                                                                                 >  1:156186/1‑251 (MQ=255)
  cGCCTTTGTCCTGATGCTCGAAGAGGTGGTGTTTTACATTCTCTACGCCCAGATGCCAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGCTGCCGATAAAATTTACTCTCGGCATGt                                                                                                                                                                                                                                >  1:233132/1‑251 (MQ=255)
       ttGTCCTGATGCTCGAAGCGGTGGTGTTTTACATTCTCTACGCCCAGATGCCAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTAt                                                                                                                                                                                                                            <  1:194242/250‑1 (MQ=255)
        tGTCCTGATGCTCGAATCGGTGGTGTTTTACATTCTCTACGCCCAGATGCCAACATCTCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTCCCATCAACCCGGTCAGCTTCCAGGCTCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCATGCATTTACACGCATCTGGGTAAGAAAGTGAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTAtg                                                                                                                                                                                                                           <  1:283845/250‑1 (MQ=255)
        tGTCCTGATGCTCGAAGCGGTGGTGTTTGACATTCTCTACGCCCAGATGCCAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATAAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTAATCGCCAGCCAAATACTGGCAGGCATTTACACGAATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTaagg                                                                                                                                                                                                                          >  2:196802/1‑248 (MQ=255)
         gTCCTGATGCTCGAAGCGGTGGTGTTTTACATTCTCTACGCCCAGATGCCAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTAtgtg                                                                                                                                                                                                                         >  2:256265/1‑251 (MQ=255)
                      aaGCGGGGGTGTTTTACATTCTCTACGCCCAGATGCCAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCCTGTTTATGTGCTCACTGGGCttt                                                                                                                                                                                                            >  1:277748/1‑251 (MQ=255)
                        gCGGTGGTGTTTTACATTCTCTACGCCCAGATGCCAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGTTTTTGCCATCAACCCGGTCAGCTTCCAGGCGCTTAGCCCGTGCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCttttt                                                                                                                                                                                                          <  2:24864/251‑1 (MQ=255)
                             ggtgTTTTACATTCTCTACGCCCAGATGCCAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTTGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGAcg                                                                                                                                                                                                      <  2:221447/250‑1 (MQ=255)
                                    tACATTCTCTACGCCCAGATGCCAACATCGCTGAACTTCTTTGCCACCAAAAACGTGCCTCATGAAATTCTCGGCTTTTCCATCAACCCGGCCAGCTTCAAGGCGCTTAACCCGTTCTGGGAGGTACTCGCCAGCCCAATACTGGCAGGAATTTACACGCATCTGGGTACCAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACAGCGGCAGc                                                                                                                                                                                              >  1:293957/1‑251 (MQ=255)
                                        ttCTCTACGCACAAATGACAACATCGCGGCACTTCTTTGACATCAATAACGTTCATAATGAAATTCTCGGATTTTCCATCAACCCGGTCAGCTTCAAGGCACTTAACACGTTCTGGGGGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGGATCTGGGTAGAAAAGGCAAAGCCCTCTCGATCCCGCTCAAATTTACTCTCCGCATGTTTACTTGCTCACTTGACTTTTTGAcggacgcgtccgcg                                                                                                                                                                                          >  2:297849/1‑240 (MQ=255)
                                         tctcTACGCCCAGATGCCAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCg                                                                                                                                                                                          <  1:259669/250‑1 (MQ=255)
                                                   cAGATGCCAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGtt                                                                                                                                                                               >  2:301673/1‑251 (MQ=255)
                                                         ccAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGa                                                                                                                                                                         <  1:144813/251‑1 (MQ=255)
                                                                                   caacaaCGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGACGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTGTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGt                                                                                                                                               <  1:221306/251‑1 (MQ=255)
                                                                                      caacGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGttt                                                                                                                                             >  1:51736/1‑250 (MQ=255)
                                                                                            gCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCTTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCATTTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTg                                                                                                                                       <  1:256370/250‑1 (MQ=255)
                                                                                              atcatTAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTGCCAGGCGCTTAACCCGTTCTGGGTGGTGCTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTg                                                                                                                                    <  1:1775/251‑1 (MQ=255)
                                                                                                   gAAAGTCTCGTTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGgtgt                                                                                                                                <  1:274250/250‑1 (MQ=255)
                                                                                                    aaaTTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTAc                                                                                                                              >  1:313041/1‑251 (MQ=255)
                                                                                                                             ggTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGTTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGt                                                                                                     >  1:246587/1‑251 (MQ=255)
                                                                                                                                 aGCTTCCCGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTACAGAGCtt                                                                                                                >  2:421782/1‑236 (MQ=255)
                                                                                                                                 aGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGTCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCtt                                                                                                                <  1:421782/236‑1 (MQ=255)
                                                                                                                                    ttCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCG‑CATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGGGTACtt                                                                                                                            <  2:284989/220‑1 (MQ=255)
                                                                                                                                    ttCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCT‑AGCATGTTTCTGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACtt                                                                                                                            >  1:284989/1‑220 (MQ=255)
                                                                                                                                                aaCCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACCCGCATCTGGGTAACAAATGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGcc                                                                                   <  1:216088/250‑1 (MQ=255)
                                                                                                                                                          gggTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCgatg                                                                           <  2:218204/248‑1 (MQ=255)
                                                                                                                                                              ggtACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCGATGATtgctg                                                                    <  1:239133/251‑1 (MQ=255)
                                                                                                                                                                        aGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCGATGATTGCTGCCCTGGTGcc                                                          <  1:40522/251‑1 (MQ=255)
                                                                                                                                                                                    gcaggcaTTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCGATGATTGCTGCCCTGGTGCCGCAGCATTTGAt                                              >  1:83181/1‑251 (MQ=255)
                                                                                                                                                                                     caggcaTTTACACGCATCTGGGTAACAACGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCCTGGCTTATCGTGCTGGTGTTCTTATTCCAGAGCTTAGGTGCACTGTTTATTAGCGCCCTTGGCCTGGCGATGATTGCTGCCCTGGTGCCGCAGCATTTGATg                                             >  2:70220/1‑251 (MQ=255)
                                                                                                                                                                                     caggcaTTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCGATGATTGCTGCCCTGGTGCCGCAGCATTTGATg                                             <  1:67436/251‑1 (MQ=255)
                                                                                                                                                                                       ggcaTTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCGATGATTGCTGCCCTGGTGCCGCAGCATTTGATggg                                           <  1:445544/251‑1 (MQ=255)
                                                                                                                                                                                                        gggTAACAAAGGCAAAGTCCTCTCGATGCCGTTAAAATTTTCTCTCGGCATGTTTATGTTCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTTGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCGATGATTTCTGCCCTGGTGCCGCAGCATTTGATGGGCTTTATTCTCGGGAtgt                          <  2:65582/251‑1 (MQ=255)
                                                                                                                                                                                                               aaaGGCAAAGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCGATGATTGCTGCCCTGGTGCCGCAGCATTTGATGGGCTTTATTCTAGGGATGTGGTTCCt                   >  2:372598/1‑251 (MQ=255)
                                                                                                                                                                                                                       aGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTttatt                                                                                                >  1:98404/1‑166 (MQ=255)
                                                                                                                                                                                                                       aGACCTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTttatt                                                                                                <  2:98404/166‑1 (MQ=255)
                                                                                                                                                                                                                          ccTCTCGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCGATGATTGCTGCCCTGGTGCCGCAGCATTTGATGGGCTTTATTCTCGGGATGTGGTTCCTGACGCAGGCTg        >  1:432175/1‑251 (MQ=255)
                                                                                                                                                                                                                              tcGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCGATGATTGCTGCCCTGGTGCCGCAGCATTTGATGGGCTTTATTCTCGGGATGTGGTTCCTGACGCAGGCTGCcgc    >  1:383913/1‑251 (MQ=255)
                                                                                                                                                                                                                               cGATGCCGATAAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCGATGATTGCTGCCCTGGTGCCGCAGCATTTGATGGGCTTTATTCTCGGGATGTGGTTCCTGACGCAGGCTGCcgcg   >  1:393400/1‑251 (MQ=255)
                                                                                                                                                                                                                                gATGCCGATAAAATTTACTCTCGGCATGTTTATGGGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGAGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCGATGATTGCTGCCCTGGCGCCGCAGCATTTGATGGGCTTTATTCTCGGGATGTGGTTCCTGACGCAGGCTGCCGCGt  >  1:270717/1‑251 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 
GTCGCCTTTGTCCTGATGCTCGAAGCGGTGGTGTTTTACATTCTCTACGCCCAGATGCCAACATCGCTGAACTTCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTCTGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGATGAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCACAAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGCCTGGCGATGATTGCTGCCCTGGTGCCGCAGCATTTGATGGGCTTTATTCTCGGGATGTGGTTCCTGACGCAGGCTGCCGCGT  >  NC_000913/3641669‑3642143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑