Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	935,214	G→A	G664D (GGC→GAC)	ftsK →	cell division DNA translocase FtsK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	935,214	0	G	A	100.0%	155.4 / NA	45	G664D (GGC→GAC)	ftsK	cell division DNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/24);  total (21/24)

AGGTCAAAGAGGGGATTGGTCCGCAGTTGCCACGACCGAAACGTATCCGCGTGCCAACTCGTCGTGAACTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAACCTGT  >  NC_000913/934974‑935439                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                  aGGTCAAAGAGGGGATTGGTCCGCAGTTGCCACGACCTAAACGTATCCGCGTGCCAACTCGTCGTGAACTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTAt                                                                                                                                                                                                                         <  2:448862/251‑1 (MQ=255)     cAAAGAGGGGATTGGTCCGCAGTTGCCACGACCGAAACGTATCCGCGTGCCAACTCGTCGTGAACTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAAc                                                                                                                                                                                                                     <  1:294466/251‑1 (MQ=255)             ggATTGGTCCGCAGTTGCCACGACCGAAACGTATCCGCGTGCCAACTCGTCGTGAACTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGAtgtg                                                                                                                                                                                                             >  1:219649/1‑251 (MQ=255)               aTTGGACCGCAGTTGCCACGCCCGAAACGTATCCGCGTGCCAACACGTCGTGAACTGGCGTCTTACGGTATTAAGCTGACATCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTAGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCTCCCAGACACAGCAGCAACGCTATGACGAACAGTATCACCATGCTGTGcc                                                                                                                                                                                                           >  2:433452/1‑251 (MQ=255)                     ccGCAGTTGCCACGACCGAAACGTATCCGCGTGCCAACTCGTCGTGAACTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTaa                                                                                                                                                                                                      <  1:166600/250‑1 (MQ=255)                      cGCAGTTGCCACGACCGAAACGTATCCGCGTGCCAACTCGTCGTGAACTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAAc                                                                                                                                                                                                    >  2:333903/1‑251 (MQ=255)                          gTTGCCACGACCGAAACGTATCCGCGTGCCAACTCGTCGTGAACTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCag                                                                                                                                                                                                <  2:126230/251‑1 (MQ=255)                                       aaaCGTATCCGCGTGCCAACTCGTCGTGAACTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGCATCAACATGATGTGCCCGTAAACGCAGAagatgcagatgc                                                                                                                                                                                   >  1:440887/1‑251 (MQ=255)                                        aaCGTATCCGCGTGCCAACTCGTCGTGAACTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGAGGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGGCCAGATACAGCAGCAACGCTATGACGAACAGTTTCAACATGATGTGCCCGTAAACGCAGAAGATGCAGAtgct                                                                                                                                                                                  <  1:26311/251‑1 (MQ=255)                                           gTATCCGCGTGCCAACTCGTCGTGAACAGCCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCATCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCg                                                                                                                                                                               >  1:125066/1‑251 (MQ=255)                                                 gcgTGCCAACTCGTCGTGAACTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCagag                                                                                                                                                                         <  1:41476/251‑1 (MQ=255)                                                              tcgtGAACTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTc                                                                                                                                                            >  1:339230/1‑251 (MQ=255)                                                                  gAACTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTc                                                                                                                                                         <  1:382443/250‑1 (MQ=255)                                                                    aCTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGt                                                                                                                                                      <  1:98471/251‑1 (MQ=255)                                                                                cGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCag                                                                                                                                                                                                >  2:359602/1‑197 (MQ=255)                                                                                cGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCag                                                                                                                                                                                                <  1:359602/197‑1 (MQ=255)                                                                                 ggTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTca                                                                                                                                         <  2:121929/251‑1 (MQ=255)                                                                                            gCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGGGCAAACTCAACAACAACGtt                                                                                                                              >  1:209129/1‑251 (MQ=255)                                                                                               cTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCCACAACAACGTTAtt                                                                                                                           >  2:357034/1‑251 (MQ=255)                                                                                                 aacaGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCGCGTCAGTTCGCCCAGACACAGCAGCACCGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGACCTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTAtt                                                                                                                           <  2:255074/248‑1 (MQ=255)                                                                                                           ggcggAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGaacaac                                                                                                               <  1:258108/251‑1 (MQ=255)                                                                                                                         cccGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAAt                                                                                                 >  2:106342/1‑251 (MQ=255)                                                                                                                              gAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGCAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACCGCAGCAACGCTCTGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCGCAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAAACGGCCCGGGCGAAttactt                                                                                            >  2:148775/1‑246 (MQ=255)                                                                                                                                aGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTtct                                                                                          <  1:56630/251‑1 (MQ=255)                                                                                                                                  cccAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCg                                                                                        <  1:232671/251‑1 (MQ=255)                                                                                                                                     aGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTg                                                                                     <  2:351693/251‑1 (MQ=255)                                                                                                                                           aTCAGTACGATTCTGGAGATCAGCACAACGATGATGAAATCGATGCGACGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTACGACGAACAGTATCAACATGATGTCCCCGTAAACGAAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCCGTTTGCGCAAACTCAACAAAAAAGTTATTCCGGAGAACAATCGGCTGGGGCGAATCCGTTTTTGCTggcaggt                                                                               >  2:443671/1‑246 (MQ=255)                                                                                                                                            tCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGAtt                                                                              >  1:55005/1‑251 (MQ=255)                                                                                                                                                  cGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAACAGGATGAACTGTCACGTCAGTTCGCCCAGCCACAGCACCAACGCTATGACGAACAGCATCAACATGATGTGCCCGTAAACGCAGACGATGCAGCTGCGGCGGAAGAGGCTGACCTGGCTCGTCAGTTTACGCAAACTCAACAACAACGTTGTTCCGGCGAACAACCGGCTGGGGCGAATACATTCTAGATGGATGGTTTTGAAt                                                                        >  2:458095/1‑251 (MQ=255)                                                                                                                                                      tCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGATATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTc                                                                    >  1:30460/1‑251 (MQ=255)                                                                                                                                                           cGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCa                                                                >  1:132308/1‑250 (MQ=255)                                                                                                                                                            gATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAAt                                                              <  1:51584/251‑1 (MQ=255)                                                                                                                                                                  tACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGc                                                        >  2:119454/1‑251 (MQ=255)                                                                                                                                                                           gatgaAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCCGCTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTggg                                               >  2:256541/1‑250 (MQ=255)                                                                                                                                                                            atgaAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGgat                                              <  2:339230/251‑1 (MQ=255)                                                                                                                                                                            atgaAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGgat                                              <  1:333903/251‑1 (MQ=255)                                                                                                                                                                                aaTCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACGTGATGGGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGACCAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGatgatg                                          <  2:360177/251‑1 (MQ=255)                                                                                                                                                                                       gcgatgcAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCcac                                    >  1:200996/1‑250 (MQ=255)                                                                                                                                                                                        cgatgcAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGCATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCacac                                  >  2:394190/1‑251 (MQ=255)                                                                                                                                                                                                       aaCTGGCACGTCATTGCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATGCTTTCTCGCTGGATGATTTTGAATTTTCGCCAATTAAAGCGTTGCTGGATGATGGTCCTCACGAACCGTTGTTTACg                   <  2:61848/251‑1 (MQ=255)                                                                                                                                                                                                        aCTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACATTATCAACATGATGTGCCCGTAAACGCATAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGGCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGTGGCGTATCCGTTCTCGCTGGATGATTTTTAATGTTCGTCAATGAAAGCGTTGCTGGATGATTGTCCACTCGAACCGTTGTTTACGc                  <  2:200256/251‑1 (MQ=255)                                                                                                                                                                                                           ggCACGTCAGTGCGCCCAGACACAGCAGAAACGCTATGACGAACAGTAGCAACATGAAGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGAAGAGGCTGAACTGGCTCGTAAGTTTGCGGAAAATCAAGAACAAAGTGATTCCGGCGAACAACCGGCTGGGGAGAATCAGTTGTCGATGGATGATTTTGAATTTTCGCCAATGAAAGAGTTACTGGATGATGGTGCACACGAACCGGTGTTTACGCCa                >  2:206688/1‑250 (MQ=255)                                                                                                                                                                                                             cACGTCAGTTCGCCCAGACATAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAAtt             <  2:145624/251‑1 (MQ=255)                                                                                                                                                                                                              acttCAGTTCGCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACTTTATTCCGTCGAACAACCGGCTGGTGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAGTTAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAAttg            <  1:443671/248‑1 (MQ=255)                                                                                                                                                                                                                        gCCCAGACACAGCAGCAACGCTATGACGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAACCTGt  <  2:219030/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                  AGGTCAAAGAGGGGATTGGTCCGCAGTTGCCACGACCGAAACGTATCCGCGTGCCAACTCGTCGTGAACTGGCGTCTTACGGTATTAAGCTGCCCTCACAGCGTGCGGCGGAAGAAAAAGCCCGTGAAGCCCAGCGCAATCAGTACGATTCTGGCGATCAGTACAACGATGATGAAATCGATGCGATGCAGCAGGATGAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTATTCCGGCGAACAACCGGCTGGGGCGAATCCGTTCTCGCTGGATGATTTTGAATTTTCGCCAATGAAAGCGTTGCTGGATGATGGTCCACACGAACCGTTGTTTACGCCAATTGTTGAACCTGT  >  NC_000913/934974‑935439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑