| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 860,260 | G→A | R783C (CGT→TGT) | ybiW ← | putative pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 860,260 | 0 | G | A | 98.4% | 220.6 / NA | 64 | R783C (CGT→TGT) | ybiW | putative pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (36/27); minor base C (1/0); total (37/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACGCACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGCCGTAGGCAGTTTACCCACTGAGCCAATGACCGCAGTAG > NC_000913/860021‑860502 | aCGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCAGCGACACACACTACCAGa > 1:23567/1‑250 (MQ=255) aTAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGAt < 2:229389/251‑1 (MQ=255) ccTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATCTTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCggg > 1:213757/1‑250 (MQ=255) ccTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCggg > 1:232682/1‑250 (MQ=255) gAAAATGAAACCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAAGTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGtttttt < 1:110151/251‑1 (MQ=255) aaaTGAAACCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCg < 2:2415/251‑1 (MQ=255) aaaTGAAACCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCg < 1:430073/251‑1 (MQ=255) aaTGAAACCGTACCAGTTTACTAACCATCTGACGCAGAGAACAACTTATCTTTCGAATGCAAGTAAGGATGACATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGCTATTCCAGCTTATGTTCAGTACAGGCGATGATATCTTCCTGAGCGT‑CTGTAGCGAGCGCTGGGACGAACCCGGACTAGCCCGCGCCCCCGACTACCAGATCGCGATACTGATCGGGCTGttttttagc > 2:444275/1‑248 (MQ=255) gAAACCGTAACAGTGCAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAAGAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCAt < 1:426518/250‑1 (MQ=255) aaaCCGTAACAGTGTAATATCAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATcc < 2:213757/251‑1 (MQ=255) cGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGCAAGGATGAAATTGATGATGTGAATGATTTAGCCAGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCcagc > 2:147873/1‑250 (MQ=255) tgtAATAACAATGTGACGCAGCGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGCTGATATCGTCCTGAGCGT‑CTGGCGAGAGCGCGGTGCAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTcg > 2:208001/1‑251 (MQ=255) gtAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTcg < 1:5767/250‑1 (MQ=255) aaTGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGaaa > 1:284007/1‑249 (MQ=255) aaTGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACg > 2:168198/1‑251 (MQ=255) gACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGCTATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGAT‑GGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGAtgttg > 2:92460/1‑251 (MQ=255) cGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGAtgttgt > 1:34176/1‑251 (MQ=255) cAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTAc > 2:313548/1‑251 (MQ=255) gagCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGCTGATGTGAATGATCTAGCCCGGCGAAGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTg > 1:184551/1‑251 (MQ=255) gCACAAATTATATTTCGAATGAAAGCAAGGATGAAATTGCTGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGTGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGCCACACACTACCAGATCGCGATCCTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGa > 2:291829/1‑250 (MQ=255) aaaaTTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAatat < 1:104378/250‑1 (MQ=255) aaaTTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATg < 1:47409/251‑1 (MQ=255) aaTTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGc < 2:208396/251‑1 (MQ=255) gTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCaa < 2:281460/251‑1 (MQ=255) tGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGANGGt > 2:405248/1‑251 (MQ=255) gAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGt > 1:416623/1‑250 (MQ=255) atgatgGGAATGATTAAGCCCGGCGCCGACGCCGCCGGGCCGAGGAGATTACAGCGTATGTTCAGTACGGGCGATGATCTCGTCCTGAGCGT‑CTGGAGCGCGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTCCCAGATCGCGATACTGCTCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGTGACACGATGCTGTACTTAATATGCGAGCCTTTCTGCACTTCAACGAAGGTACgcagc > 2:345020/1‑250 (MQ=255) tgtgAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGat > 1:311144/1‑251 (MQ=255) ccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTg > 2:26264/1‑137 (MQ=255) ccGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTg < 1:26264/137‑1 (MQ=255) gCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTc < 1:60357/251‑1 (MQ=255) gccgccGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATTCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGt < 1:413830/251‑1 (MQ=255) ccGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCg < 2:311144/251‑1 (MQ=255) gaggagATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGtt > 1:266381/1‑251 (MQ=255) gaggagATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGtt > 1:131085/1‑251 (MQ=255) aggagATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTg > 2:72291/1‑251 (MQ=255) gaTTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCgg > 2:115444/1‑251 (MQ=255) cAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCAGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTc > 2:454291/1‑250 (MQ=255) tGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTg > 2:368240/1‑251 (MQ=255) gtaaagcaCGGGCGATGATATGCTCCTGAGCGA‑CTAGAGAGAGCACGGTGACTAACGGGGAATGGCTCGCGACACACACTACCAGATCGCGAACCTGATCGGGATGTTTTTTCGCATCAAGCAGCGTTCCGCGGGAAACGATGTTGTACTGAATAAGCAAGTCTTTATGCACTGCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCCTTGCAGGAATCacgttgtga < 2:305846/244‑9 (MQ=255) ggagtggCGATGATATCGTCCTGA‑TGTGCTGGAGAGAGCGCGGTGAAGAACGCGGAATATCCCGCGACACACACTACCAGATCGCGATACTGTTCCGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATCTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCATCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCGCCAGCGTTGCCGGATTCAGTTTCTGGtttag < 2:20214/246‑4 (MQ=255) aCGGGCGAGGATATCGTCCTGAGCGT‑CTGGAGAGAGCGAGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGAGATACTGATAGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGAACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGATTGTCAGATTCGTTATCCAGCGTTGCCGGATTAAGTTTCTGGTTGAga > 1:237877/1‑249 (MQ=255) gatgatATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACAcgc > 2:442642/1‑251 (MQ=255) atgatATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCCGCAGGATCATCAGTTTCTGCTTGTCAGCTTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACAcgcc > 2:129056/1‑251 (MQ=255) gatATCGTCCTGAGCGT‑CTGGAGATAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGGACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCTGATTCGTTCTCCAGCGTTGCCGTATTCAGTTTCTGGTTGAGCAACAcgccgc < 1:305797/251‑1 (MQ=255) cttatCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTAt < 2:349316/150‑1 (MQ=255) cttatCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTAt > 1:349316/3‑152 (MQ=255) gAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGAt > 2:241370/1‑115 (MQ=255) gAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGAt < 1:241370/115‑1 (MQ=255) gAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCt < 2:232682/251‑1 (MQ=255) gagagaGCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGCCGTAGGc > 1:235906/1‑251 (MQ=255) gagagCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGCCGTAGGc > 2:448619/1‑249 (MQ=255) agagCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGTGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCTGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGGCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACTCGCCGCCGAGAATCGCTGCCGTAGGCAGt < 1:424536/251‑1 (MQ=255) agagCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGCCGTAGGCAGt > 1:334800/1‑251 (MQ=255) gagCGCGGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGCCGTAGGCAGtt < 2:131085/251‑1 (MQ=255) cgcgGTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGCCGTAGGCAGTTTAc < 1:448619/251‑1 (MQ=255) gTGAAGAACGCGGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGCCGTAGGCAGTTTACCCAc < 1:390435/251‑1 (MQ=255) aaagcgGAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGACGTAGGCAGTTTACCCACTGAGcc > 2:391702/4‑251 (MQ=255) cgcgGAATAGCCCGCGACACACACTACCAGATCGCGTTACTGATCGGGAAGTTTTTTCCCATCCAGCAGCGTTTCGCGGGAAACGCTGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGCGCAACACGCTGCCGAGAATCGCTGCCGTAGGCAGTTTACCCACTGAGCCa > 2:314334/1‑250 (MQ=255) ggAATAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGCCGTAGGCAGTTTACCCACTGAGCCAATg > 1:303499/1‑250 (MQ=255) aTAGCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGCTCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGCCGTAGGCAGTTTACCCACTGAGCCAATGACCg > 1:339918/1‑251 (MQ=255) gCCCGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGCCGTAGGCAGTTTACCCACTGAGCCAATGACCGCAg < 1:256306/251‑1 (MQ=255) cccGCGACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGCCGTAGGCAGTTTACCCACTGAGCCAATGACCGCAGt > 1:214964/1‑251 (MQ=255) cgcgACACACACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGCCGTAGGCAGTTTACCCACTGAGCCAATGACCGCAGTAg < 1:313548/251‑1 (MQ=255) | ACGCTCATAGGTGTGCTCCTGGCTCGAAAATGAAACCGTAACAGTGTAATAACAATGTGACGCAGAGCACAAATTATATTTCGAATGAAAGTAAGGATGAAATTGATGATGTGAATGATTTAGCCCGGCGACGACGCCGCCGGGCCGAGGAGATTACAGCATATGTTCAGTACGGGCGATGATATCGTCCTGAGCGT‑CTGGAGAGAGCGCGGTGAAGAACGCGGAATAGCCCGCGACACGCACTACCAGATCGCGATACTGATCGGGATGTTTTTTCGCATCCAGCAGCGTTTCGCGGGAAACGATGTTGTACTGAATATGCCAGCCTTTATGCACTTCAAAGAAGGTACGCAGCAGGATCATCAGTTTCTGCTTGTCAGATTCGTTCTCCAGCGTTGCCGGATTCAGTTTCTGGTTGAGCAACACGCCGCCGAGAATCGCTGCCGTAGGCAGTTTACCCACTGAGCCAATGACCGCAGTAG > NC_000913/860021‑860502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |